Global gene expression profiling of<i>Ehrlichia ruminantium</i>at different stages of development

Pruneau, Ludovic; Emboule, Loïc; Gely, Pryscellia; Marcelino, Isabel; Mari, Bernard; Pinarello, Valérie; Sheikboudou, Christian; Martinez, Dominique; Lefrançois, Thierry; Meyer, Damien; Vachiéry, Nathalie

doi:10.1111/j.1574-695x.2011.00901.x

Cited by 23 publications

(41 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Transcriptional profiling comparing the equivalent intracellular ("RB") versus infectious ("EB") forms in Ehrlichia ruminatum also found that an APH_1235 ortholog, CDS_00640, was upregulated in the infectious EB form (30). Further evidence of the development of the DC form is provided by the detection of multiple chaperones and stress response proteins (see Table S1 in the supplemental material).…”

Section: Discussionmentioning

confidence: 90%

Postgenomic Analyses Reveal Development of Infectious Anaplasma phagocytophilum during Transmission from Ticks to Mice

2012

View full text Add to dashboard Cite

Obligate intracellular bacteria of the Rickettsiales order have evolved to colonize both arthropod and mammalian hosts, but few details are known about the bacterial adaptations that occur during transmission from blood-feeding arthropods to mammals. Here we apply proteomics and transcriptome sequencing to Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis, in Ixodes scapularis tick salivary glands, to detect proteins or genes expressed by the pathogen during transmission feeding by the tick. We detected expression of 139 genes, representing 11% of the open reading frames (ORFs) in the A. phagocytophilum genome. The predominant categories of proteins were ribosomal proteins, cell surface proteins, chaperones, and uncharacterized proteins. There was no evidence of DNA replication enzymes, suggesting that most of the A. phagocytophilum cells were no longer dividing. Instead, protein expression reflected conversion to the extracellular, infectious "dense-core" (DC) form. High expression of a DC-specific marker, APH_1235, further suggested this developmental transition in ticks. We showed that blocking APH_1235 with antibodies reduced A. phagocytophilum infection levels in mammalian cell culture. This work represents a starting point for clarifying essential proteins expressed by A. phagocytophilum during transmission from ticks to mammals and demonstrates that the abundantly expressed, DC-associated APH_1235 protein is important during in vivo infection by A. phagocytophilum.

show abstract

Section: Discussionmentioning

confidence: 90%

Postgenomic Analyses Reveal Development of Infectious Anaplasma phagocytophilum during Transmission from Ticks to Mice

2012

View full text Add to dashboard Cite

show abstract

“…Bacterial replication itself needs nutrients from the host and occurs thanks to the subversion of host cell machineries (e.g., manipulation of autophagy or cholesterol pathways; Barnewall et al, 1997; Niu et al, 2008; Xiong et al, 2008; Wakeel et al, 2009; Liu et al, 2012; Xiong and Rikihisa, 2012). Recent transcriptomic studies on E. ruminantium infectious form provide evidence of the expression of genes involved in the bacterial defense and invasion systems, showing that Ehrlichia allocates a significant part of its energetic resources to virulent purposes (Pruneau et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Economic Game Theory to Model the Attenuation of Virulence of an Obligate Intracellular Bacterium

Tago

Meyer

2016

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

Diseases induced by obligate intracellular pathogens have a large burden on global human and animal health. Understanding the factors involved in the virulence and fitness of these pathogens contributes to the development of control strategies against these diseases. Based on biological observations, a theoretical model using game theory is proposed to explain how obligate intracellular bacteria interact with their host. The equilibrium in such a game shows that the virulence and fitness of the bacterium is host-triggered and by changing the host's defense system to which the bacterium is confronted, an evolutionary process leads to an attenuated strain. Although, the attenuation procedure has already been conducted in practice in order to develop an attenuated vaccine (e.g., with Ehrlichia ruminantium), there was a lack of understanding of the theoretical basis behind this process. Our work provides a model to better comprehend the existence of different phenotypes and some underlying evolutionary mechanisms for the virulence of obligate intracellular bacteria.

show abstract

“…To our knowledge, this is the first study to investigate the global gene expression analysis of E. ruminantium Welgevonden in different developmental stages using transcriptome or RNA-sequencing. Available studies are based on microarray analysis (Emboulé et al, 2009;Pruneau et al, 2012). In general, microarray results are consistent with RNA-seq data, but the RNA-seq technique is more sensitive (Wang et al, 2009).…”

Section: Introductionmentioning

confidence: 88%

Transcriptomic analysis of Ehrlichia ruminantium during the developmental stages in bovine and tick cell culture

Tjale

Pretorius

Josemans

et al. 2018

Ticks and Tick-borne Diseases

View full text Add to dashboard Cite

The use of bioinformatics tools to search for possible vaccine candidates has been successful in recent years. In an attempt to search for additional vaccine candidates or improve the current heartwater vaccine design, a genome-wide transcriptional profile of E. ruminantium (Welgevonden strain) replicating in bovine endothelial cells (BA886) and Ixodes scapularis embryonic tick cells (IDE8) was performed. The RNA was collected from the infective extracellular form, the elementary bodies (EBs) and vegetative intracellular form, reticulate bodies (RBs) and was used for transcriptome sequencing. Several genes previously implicated with adhesion, attachment and pathogenicity were exclusively up-regulated in the EBs from bovine and tick cells. Similarly, genes involved in adaptation or survival of E. ruminantium in the host cells were up-regulated in the RBs from bovine cells. Thus, it was concluded that those genes expressed in the EBs might be important for infection of mammalian and tick host cells and these may be targets for both cell and humoral mediated immune responses. Alternatively, those exclusively expressed in the RBs may be important for survival in the host cells. Exported or secreted proteins exclusively expressed at this stage are ideal targets for the stimulation of cytotoxic T-lymphocyte (CTL) immune responses in the host.

show abstract

Global gene expression profiling ofEhrlichia ruminantiumat different stages of development

Cited by 23 publications

References 21 publications

Postgenomic Analyses Reveal Development of Infectious Anaplasma phagocytophilum during Transmission from Ticks to Mice

Postgenomic Analyses Reveal Development of Infectious Anaplasma phagocytophilum during Transmission from Ticks to Mice

Economic Game Theory to Model the Attenuation of Virulence of an Obligate Intracellular Bacterium

Transcriptomic analysis of Ehrlichia ruminantium during the developmental stages in bovine and tick cell culture

Contact Info

Product

Resources

About