Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low‐­intensity electromagnetic field

Abstract

A polysaccharide of Irpex lacteus, a white‐rot fungus with lignocellulose‐degrading activities, has been used as a commercial medicine for nephritis treatment. Previously, a low‐intensity electromagnetic field (LI‐EMF) was found to increase the biomass and polysaccharide content of Irpex lacteus and induce twists on the cell surface. In this study, RNA‐sequencing (RNA‐seq) technology was used to analyze the underlying mechanism of LI‐EMF's influence on Irpex lacteus. We identified 3268, 1377, and 941 differentially expressed genes (DEGs) in the LI‐EMF‐treated samples at recovery times of 0 h, 3 h, and 6 h, respectively, indicating a significant decline in the influence of the LI‐EMF treatment on Irpex lacteus with the passage of recovery time. Moreover, 30 upregulated and 14 downregulated DEGs overlapped in the LI‐EMF‐treated samples at the recovery times of 0 h, 3 h, and 6 h, implying the important lasting effects of LI‐EMF. The reliability of the RNA‐seq data were validated by quantitative real‐time PCR (qRT‐PCR). The DEGs related to transcription factors, cell proliferation, cell wall, membrane components, amino acid biosynthesis and metabolism, and polysaccharide biosynthesis and metabolism were significantly enriched in the LI‐EMF‐treated samples. The experiments confirmed that the LI‐EMF treatment significantly increased the content of amino acids with a considerable increase in the content of essential amino acids. Therefore, the global gene expression changes explained the pleiotropic effects of Irpex lacteus induced by the LI‐EMF treatment. These findings provide the requisite data for the appropriate design and application of LI‐EMF in the fermentation of microorganisms to increase production. Bioelectromagnetics. 40:104–117, 2019. © 2019 Bioelectromagnetics Society

Genes and Cancer under Magnetic Control

“…Fifth, the concentration dependences of the DNA yield are almost identical for magnetic and nonmagnetic ions (Figs. [1][2][3][4][5]; it supports the idea that after switching on radical pair mechanism the subsequent ions, which enter in enzymatic site, are seemingly sitting on the pyrophosphate residue and do not interfere in the radical pair mechanism.…”

“…The exposure of the rats to long-term radiofrequency radiation at 900, 1800, and 2100 MHz results to the oxidative DNA damage and single-strand DNA breaks in the frontal lobe of brain tissues [4]. In a white-rot fungus Irpex lacteus used in medicine and subjected to the electromagnetic treatment there were identified by RNA-and realtime PCR sequencing technologies 3268, 1377, and 941 differentially expressed genes [5]; it was concluded that the global gene expression changes induced by electromagnetic treatment explained the pleiotropic medical effects of Irpex lacteus. It was pointed out that a large number of studies have shown that microwave radiation can cause a series of adverse reactions in the central nervous system, including sleep disorders in addition to learning and memory impairments [6].…”

Hinokitiol inhibits Aspergillus fumigatus by interfering with the cell membrane and cell wall

“…The Control (DMSO treated) and Test (HK treated) samples were harvested after incubation for 36 h. The total RNA was prepared using the Fungal Total RNA198 Isolation Kit (B518629, Sangon Biotech, Shanghai, China), and reverse transcribed with HiScript III RT SuperMix (Vazyme, Nanjing, China) according to the manufacturer’s instructions. The PCR method was based on previous studies ( Sun et al, 2019 ). Primers used for the qRT-PCR are listed in Supplementary Table S2 .…”

Hinokitiol alters Gene Expression in Aspergillus fumigatus, protects against fungal keratitis by Reducing Fungal Load, LOX-1, Proinflammatory cytokines and Neutrophil Infiltration

“…RNA samples were reverse transcribed using HiScript III RT SuperMix (Vazyme, Nanjing, China) to produce cDNA template. The PCR method was based on previous studies (61). The primer details are listed in S3 Table.…”

“…The total RNA was prepared using the Fungal Total RNA Isolation Kit (B518629, Sangon Biotech, Shanghai, China), and reverse transcribed with HiScript III RT SuperMix (Vazyme, Nanjing, China) according to the manufacturer's instructions. The PCR method was based on previous studies (61). Primers used for the qRT-PCR are listed in S1 Table.…”