Global DNA methylation analysis reveals miR-214-3p contributes to cisplatin resistance in pediatric intracranial nongerminomatous malignant germ cell tumors

Hsieh, Tsung Han; Liu, Yun Ru; Chang, Ting; Liang, Muh Lii; Chen, Hsin Hung; Wang, Hsei Wei; Yen, Yun; Wong, Tai-Tong

doi:10.1093/neuonc/nox186

Cited by 21 publications

(11 citation statements)

References 46 publications

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“…DNA hydroxymethylation is a stable epigenetic modification that plays unique regulatory roles in various physiopathological processes [4]. 5-Hydroxymethylcytosine (5 hmC, the marker of hydroxymethylation) is distributed in various different tumours or different development stages, suggesting that it may have key roles in different cancers and disorders [5][6][7]. The distribution of 5 hmC can be found at the enhancer, promoter, transcription start site (TSS), gene body, 3ʹUTR or intragenic region, so the function and mechanism of 5 hmC need to be further investigated [8].…”

Section: Introductionmentioning

confidence: 99%

Aberrant hydroxymethylation ofANGPTL4is associated with selective intrauterine growth restriction in monochorionic twin pregnancies

et al. 2020

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Selective intrauterine growth restriction (sIUGR) is a severe complication in monochorionic (MC) twin pregnancies, and it carries increased risks of poor prognosis. Current data suggest that vascular anastomoses and unequal placental sharing may be the key contributor to discordant foetal growth. While MC twins derive from a single zygote and have almost identical genetic information, the precise mechanisms remain unknown. DNA hydroxymethylation is a newly discovered epigenetic feature associated with gene regulation and modification. Here, we investigate discordant hydroxymethylation patterns between two placental shares of sIUGR and analyse the potential role of aberrant hydroxymethylation of angiopoietin-like 4 (ANGPTL4) in placental dysplasia. Hydroxymethylation DNA immunoprecipitation (hMeDIP)-chip and mRNA sequencing were performed to identify hydroxymethylation-associated genes. Real-time qPCR, western blotting, and immunohistochemistry were used to confirm ANGPTL4 expression. The mechanisms regulating ANGPTL4 were investigated by cell migration assay, invasion assay, viability assay, and apoptotic ratio assays, western blotting and hMeDIP-qPCR. Decreased ANGPTL4 was detected in the smaller placental shares of sIUGR. ANGPTL4 knockdown suppressed trophoblast invasiveness and migration, which possibly occurred through hypoxia inducible factor 1α (HIF-1α) and HIF-1 signalling pathway. Hypoxia leads to aberrant expression of ANGPTL4 and HIF-1α, positively correlated with their aberrant hydroxymethylation levels in promoter regions. Aberrant hydroxymethylation of ANGPTL4 may contribute to placental impairment by the HIF-1 signalling pathway in smaller placental shares of sIUGR.

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Section: Introductionmentioning

confidence: 99%

Aberrant hydroxymethylation ofANGPTL4is associated with selective intrauterine growth restriction in monochorionic twin pregnancies

et al. 2020

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“…miR-214 is expressed widely in various tissues and cells, targeting a variety of genes involved in cancer development, the immune system, differentiation of skeletal muscle cells, protection of the heart from ischemic injury, and inhibition of angiogenesis 41, 42, 43, 44. Recently, it has received heightened interest, as it may function as both a diagnostic marker and therapeutic target for bone disease.…”

Section: Discussionmentioning

confidence: 99%

AAV-Anti-miR-214 Prevents Collapse of the Femoral Head in Osteonecrosis by Regulating Osteoblast and Osteoclast Activities

Wang

Sun

Ling

et al. 2019

Molecular Therapy - Nucleic Acids

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Osteonecrosis of the femoral head, an intractable but common disease that eventually triggers collapse of the femoral head, is characterized by increased osteoclast activity and markedly decreased osteoblast activity in the necrotic region of the femoral head. MicroRNA (miRNA)-214 (miR-214) may play important roles in vertebrate skeletal development by inhibiting osteoblast function by targeting activating transcription factor 4 (ATF4) and promoting osteoclast function via phosphatase and tensin homolog (PTEN). This study revealed significantly increased levels of miR-214 in necrotic regions, with commensurate changes in the numbers of its target cells (both osteoblasts and osteoclasts). To investigate whether targeting miR-214 could prevent femoral head collapse, we constructed an adeno-associated virus (AAV)-associated anti-miR-214 (AAV-anti-miR-214) and evaluated its function in vivo. AAV-anti-miR-214 promoted osteoblast activity and diminished osteoclast activity, effectively preventing collapse of the femoral head in a rat model of osteonecrosis.

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“…The flow cytometry results revealed a cell cycle arrest at the S and G2/M phases [ 60 ]. Both miR-1 [ 61 ] and miR-214 [ 62 , 63 ] were silenced by epigenetic mechanisms during the development and cancer proliferation. The miR-1 involves the initiation of hepatocellular carcinoma whilst miR-214 contributes to drug resistance in renal cell carcinoma and pediatric intracranial non-germinomatous malignant germ cell tumors.…”

Section: Epigenetic Mirna In Other Asbestos-related Diseases Possible...mentioning

confidence: 99%

Microbiomes, Epigenomics, Immune Response, and Splicing Signatures Interplay: Potential Use of Combination of Regulatory Pathways as Targets for Malignant Mesothelioma

Damane

Mkhize‐Kwitshana

Mehrotra³

et al. 2022

IJMS

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Malignant mesotheliomas (MM) are hard to treat malignancies with poor prognosis and high mortality rates. This cancer is highly misdiagnosed in Sub-Saharan African countries. According to literature, the incidence of MM is likely to increase particularly in low-middle-income countries (LMICs). The burden of asbestos-induced diseases was estimated to be about 231,000 per annum. Lack of awareness and implementation of regulatory frameworks to control exposure to asbestos fibers contributes to the expected increase. Exposure to asbestos fibers can lead to cancer initiation by several mechanisms. Asbestos-induced epigenetic modifications of gene expression machinery and non-coding RNAs promote cancer initiation and progression. Furthermore, microbiome–epigenetic interactions control the innate and adaptive immunity causing exacerbation of cancer progression and therapeutic resistance. This review discusses epigenetic mechanisms with more focus on miRNAs and their interaction with the microbiome. The potential use of epigenetic alterations and microbiota as specific biomarkers to aid in the early detection and/or development of therapeutic targets is explored. The advancement of combinatorial therapies to prolong overall patient survival or possible eradication of MM especially if it is detected early is discussed.

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Global DNA methylation analysis reveals miR-214-3p contributes to cisplatin resistance in pediatric intracranial nongerminomatous malignant germ cell tumors

Cited by 21 publications

References 46 publications

Aberrant hydroxymethylation ofANGPTL4is associated with selective intrauterine growth restriction in monochorionic twin pregnancies

Aberrant hydroxymethylation ofANGPTL4is associated with selective intrauterine growth restriction in monochorionic twin pregnancies

AAV-Anti-miR-214 Prevents Collapse of the Femoral Head in Osteonecrosis by Regulating Osteoblast and Osteoclast Activities

Microbiomes, Epigenomics, Immune Response, and Splicing Signatures Interplay: Potential Use of Combination of Regulatory Pathways as Targets for Malignant Mesothelioma

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