Global dissemination of extensively drug‐resistant carbapenemase‐producing <scp>E</scp>nterobacteriaceae: clinical perspectives on detection, treatment and infection control

Tängdén, Thomas; Giske, Christian G.

doi:10.1111/joim.12342

Cited by 289 publications

(245 citation statements)

References 62 publications

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“…Moreover, the heterogeneity of carbapenemase classes and types leads to a 55 multiplicity of diverse carbapenem hydrolytic efficiencies and resistance phenotypes 56 (Hrabák et al, 2014, Tängdén andGiske, 2015). Since carbapenem resistance mediated 57 by carbapenemase production is continuously rising in Enterobacteriaceae, rapid, 58 inexpensive, and reliable methods are urgently needed to identify CPE (Dortet et al, 59…”

Section: Mimet-d-15-00845 Revisedmentioning

confidence: 99%

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Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

Pires

Tinguely

Bodmer

et al. 2016

Journal of Microbiological Methods

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Section: Mimet-d-15-00845 Revisedmentioning

confidence: 99%

“…The continuous worldwide expansion of carbapenemase-producing Enterobacteriaceae 50 (CPE) is a serious concern as infections caused by these pathogens have an increased 51 mortality, morbidity, and associated health-care costs (Tängdén and Giske, 2015). 52…”

Section: Mimet-d-15-00845 Revisedmentioning

confidence: 99%

Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

Pires

Tinguely

Bodmer

et al. 2016

Journal of Microbiological Methods

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“…Carbapenemase-producing Klebsiella pneumoniae (KPC) has sparked interest due to the high antibiotic resistance that causes increased mortality compared to other strains [2,3]. Multiple outbreaks have been reported worldwide that have led to the application of several detection and control strategies [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

Zúñiga

Cruz

Pérez

et al. 2016

J Infect Dev Ctries

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Introduction: Carbapenemase-producing Klebsiella pneumoniae (KPC) outbreaks may cause a huge economical burden on developing countries. Furthermore, KPC can be challenging to detect. We describe the laboratory strategy for the detection of KPC from 2011 to 2013 in a tertiary care hospital in Central America with approximately 1,000 beds. Methodology: A retrospective analysis of a clinical laboratory database was done to determine the pragmatic application of the combined-disk boronic acid test during a KPC outbreak in Panama. A total of 1,026 Klebsiella pneumoniae isolates were found, of which 133 were positive for KPC. The strategy during two phases was described according to the test employed as a confirmatory test for KPC. After the K. pneumoniae isolates were detected by the VITEK 2 system, blaKPC polymerase chain reaction (PCR) and the combined-disk boronic acid test were employed as a confirmatory test during phase one. The combined-disk boronic acid test was employed as a confirmatory test for KPC during phase two. Results: The sensitivity, specificity, positive predictive value, and negative predictive value of the boronic acid test were 100%, 97%, 91%, and 100%, respectively, when blaKPC PCR was employed as a confirmatory test during the start of the outbreak. Afterwards, modified VITEK 2 system parameters resulted in 116 suspicious KPC samples and the boronic acid test confirmed 102 isolates. Conclusions: The use of an automated bacterial identification system and the boronic acid test for the detection of KPC was an effective and low-cost strategy for a clinical laboratory in Panama during an outbreak.

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“…Prompt and adequate treatment is crucial. The management of these life-threatening infections and treatment decisions should be guided by reliable antimicrobial susceptibility testing (Gagliotti et al, 2014;Tängdén, Giske, 2015).…”

Section: Introductionmentioning

confidence: 99%

β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil

Leite

Oizumi

Caleffi-Ferracioli

et al. 2017

Braz. J. Pharm. Sci.

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The present study evaluated the antimicrobial susceptibility profile, b-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and b-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic extended spectrum b-lactamase (ESBL) test was positive in 53.8% of the K. pneumoniae isolates, 100.0% of the E. coli isolates, and 100.0% of the E. aerogenes isolates. Phenotypic and genotypic testing of K. pneumoniae carbapenemase (KPC) was positive in 50.0% of the K. pneumoniae isolates. Phenotypic and genotypic testing showed that none of the P. aeruginosa or Acinetobacter spp. isolates were positive for metallo-b-lactamase (MBL). The bla OXA gene was detected only in Acinetobacter spp. The lowest genetic diversity, determined by ERIC-PCR, was observed among the KPC-producing K. pneumoniae isolates and OXA-producing Acinetobacter spp. isolates, indicating the inadequate dissemination control of multidrug-resistant bacteria in this hospital environment.

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Global dissemination of extensively drug‐resistant carbapenemase‐producing Enterobacteriaceae: clinical perspectives on detection, treatment and infection control

Abstract: Abstract. T€ angd en T,

Cited by 289 publications

References 62 publications

Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

Comparison of the in-house made Carba-NP and Blue-Carba tests: Considerations for better detection of carbapenemase-producing Enterobacteriaceae

The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil

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