Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray

Zhou, Guixuan; Wang, Songbo; Wang, Zhonggang; Zhu, Xiaotong; Shu, Gang; Liao, Weiyi; Yu, Kaifan; Gao, Ping; Xi, Qianyun; Wang, Xiu-Qi; Zhang, Yongliang; Li, Yuan; Jiang, Qingyan

doi:10.1016/j.meatsci.2010.05.031

Cited by 62 publications

(75 citation statements)

References 50 publications

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“…This is consistent with the observation that porcine subcutaneous and intramuscular adipocytes express BMP4 mRNA and that BMP4 is secreted from porcine adipose tissue. [43][44][45] The novel observation that BMP4 mRNA was upregulated during differentiation in the present study further supports the hypothesis that BMP4 may regulate adipogenesis in the pig.…”

Section: Discussionsupporting

confidence: 88%

The proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°Celsius compared to euthermic conditions in pigs

et al. 2014

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Given similarities in metabolic parameters and cardiovascular physiology, the pig is well positioned as a biomedical model for metabolic disease and obesity in humans. Better understanding molecular mechanisms governing porcine adipocyte hyperplasia may provide insight into the regulation of adipose tissue development that is useful both when considering the pig as a commodity and when extrapolating porcine data to human disease. Primary cultures of pig stromal-vascular cells have served as a useful tool for investigating factors that regulate preadipocyte proliferation and differentiation. However, such cultures have generally been maintained at 37°C in vitro despite euthermia being 39°C in pigs. To address potential concerns about the physiological relevance of culturing primary pig preadipocytes under what would be hypothermic conditions in vivo, the objective of this study was to investigate the effect of culture temperature on the proliferation and differentiation of pig preadipocytes in primary culture. Culturing primary preadipocytes at 37 rather than 39°C decreases their proliferation rates based upon cleavage of the tetrazolium salt, MTT (P < 0.001), reduction of resazurin (P < 0.001), and daily cell counts (P < 0.001). Likewise, culturing primary porcine preadipocytes at 37°C suppressed their adipogenic potential based upon monitoring adipogenesis morphologically, biochemically, and via the expression of mRNA encoding adipogenic marker genes. Collectively, these data indicate the proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°C compared to normal body temperature of pigs. This may confound investigation of factors that impact adipocyte hyperplasia in the pig.

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Section: Discussionsupporting

confidence: 88%

The proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°Celsius compared to euthermic conditions in pigs

et al. 2014

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“…The differential gene expression pattern of key lipogenic factors PPARG, CEBPA and FABP4 between the SAT and the LL muscle, regarding genotype and diet effects as well as the magnitude of responses, has already been reported (Zhou et al, 2010;Madeira et al, 2013). One can speculate that the lipogenic and lipolytic pathways are downregulated in intramuscular adipocytes compared with fat cells from other depots, in particular SAT.…”

Section: Discussionmentioning

confidence: 76%

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Lopes

Costa

et al. 2014

Animal

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The production of pork with high amounts of intramuscular fat (IMF) without an increase in subcutaneous fat is highly desirable for the pig industry and consumers. Herein, we question the impact of dietary protein reduction (18% v. 13%) on fat deposition in the subcutaneous adipose tissue (SAT) and longissimus lumborum (LL) muscle using genetically diverse pigs for body fatness (lean v. fat). A clear effect of genotype was observed on plasma insulin (P = 0.004) and leptin (P < 0.001), as well as on backfat thickness (P < 0.001), with the fat pigs having higher values. Accordingly, IMF was higher in the fat pigs, when compared with their lean counterparts (P = 0.003), which was supported by enlarged adipocytes (P < 0.001). The area of lipid droplets within the LL fibres (P = 0.039) and extramyocellular lipids number (P = 0.017) were increased in pigs fed reduced protein diets, regardless of genotype, which is consistent with higher levels of plasma triacylglycerols (P = 0.002). The gene-expression pattern of lipogenic factors in the SAT was distinct from the LL muscle. In the SAT, PPARG expression was similar among genotypes (P > 0.05), whereas in the LL muscle it was higher in the lean pigs (P = 0.023), especially when fed on low protein diet (P = 0.057). The CEBPA and FABP4 mRNA levels were increased in the SAT of fat pigs (P < 0.001), without changes in the LL muscle (P > 0.05). The influence of diet on FABP4 expression in the SAT was dependent on pig's genetic background (P = 0.005). In conclusion, fat deposition was clearly influenced by genotype and, to a lesser extent, by dietary protein level, the SAT being more sensitive than the LL muscle. One can speculate that the pathways involved in lipid metabolism are downregulated in intramuscular adipocytes when compared with SAT fat cells. This result might be a direct consequence of the relatively low proportion of adipocytes found in the LL muscle.

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“…Our previous study also indicated that compared with lipids accumulated in s.c. adipocytes at late stage of differentiation in vitro , less lipids were accumulated in porcine i.m. adipocytes and that the significant difference occurred on the 6th differentiation day [9]. In addition, studies on bovine adipocytes had shown that i.m.…”

Section: Introductionmentioning

confidence: 99%

“…adipocytes and that PDK4, a gene involved in glycolysis, was rich in i.m. adipocytes [9]. These findings suggested that these enzymes genes might account for the differences of lipid accumulation between i.m.…”

Section: Introductionmentioning

confidence: 99%

Glucose Utilization, Lipid Metabolism and BMP-Smad Signaling Pathway of Porcine Intramuscular Preadipocytes Compared with Subcutaneous Preadipocytes

Wang

Zhou

Wang

et al. 2013

Cell Physiol Biochem

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Background/Aims: We previously reported that porcine intramuscular (i.m.) preadipocytes were different from subcutaneous (s.c.) preadipocytes on cell differentiation and lipid accumulation, but the underlying mechanisms remained unknown. The paper aims to investigate the underlying mechanisms by comparing the differences between i.m. and s.c. preadipocytes in glucose utilization, lipid metabolism, and the role of BMP signaling pathway. Methods: Experiments were performed in porcine primary i.m. and s.c. preadipocytes in culture. The mRNA and protein expression patterns were determined respectively by Quantitative real-time PCR and Western blot. Cytosolic triglycerides were examined by triglyceride assay. Results: The i.m. preadipocytes consumed more glucose by expression of GLUT1 and s.c. preadipocytes mainly utilized exogenic fatty acids for lipid synthesis by expression of LPL and FAT. Meanwhile, the expression of genes related to lipogenesis and lipolysis in s.c. preadipocytes increased more quickly than those in i.m. preadipocytes. The expression patterns of the genes involved in BMP-Smad signaling pathway were consistent with those of the genes participated in adipocytes differentiation in both i.m. and s.c. preadipocytes. Exogenous BMP2 significantly increased, whereas Noggin and Compound C, remarkablely decreased the triglycerides content in i.m. preadipoytes, without affecting s.c. preadipocytes. BMP2 shRNA significantly reduced the mRNA levels of the downstream genes of BMP-Smad signaling pathway and PPARγ in both i.m. and s.c. preadipocytes. Conclusion: These findings suggested that the differentiation and lipid accumulation differences between i.m. and s.c. preadipocytes might be caused by the different manners of glucose utilization, lipid metabolism and the BMP-Smad signaling pathway. The special feature of i.m. adipocytes implied that these cells might be a potential target for treatment of diabetes.

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Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray

Cited by 62 publications

References 50 publications

The proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°Celsius compared to euthermic conditions in pigs

The proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°Celsius compared to euthermic conditions in pigs

Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction

Glucose Utilization, Lipid Metabolism and BMP-Smad Signaling Pathway of Porcine Intramuscular Preadipocytes Compared with Subcutaneous Preadipocytes

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