Global characterization of megakaryocytes in bone marrow, peripheral blood, and cord blood by single-cell RNA sequencing

Zhang, Weilong; Yan, Changjian; Liu, Xiaoni; Yang, Ping; Wang, Jing; Chen, Yingtong; Liu, Weiyou; Li, Shaoxiang; Zhang, Xiuru; Dong, Gaochao; Xue, He; Yuan, Xiaoliang; Jing, Hongmei

doi:10.1038/s41417-022-00476-z

Cited by 9 publications

(11 citation statements)

References 53 publications

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“…Furthermore, it had no impact on the levels of WBCs and RBCs. MKs are mainly derived from the BM [ 57 ]. H&E staining of and cytoploidy results for the BM showed that ICGA-A could increase the number of MKs in the BM and promote MK differentiation and maturation.…”

Section: Discussionmentioning

confidence: 99%

An Innovative Inducer of Platelet Production, Isochlorogenic Acid A, Is Uncovered through the Application of Deep Neural Networks

Yi,

Luo,

Liao

et al. 2024

Biomolecules

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(1) Background: Radiation-induced thrombocytopenia (RIT) often occurs in cancer patients undergoing radiation therapy, which can result in morbidity and even death. However, a notable deficiency exists in the availability of specific drugs designed for the treatment of RIT. (2) Methods: In our pursuit of new drugs for RIT treatment, we employed three deep learning (DL) algorithms: convolutional neural network (CNN), deep neural network (DNN), and a hybrid neural network that combines the computational characteristics of the two. These algorithms construct computational models that can screen compounds for drug activity by utilizing the distinct physicochemical properties of the molecules. The best model underwent testing using a set of 10 drugs endorsed by the US Food and Drug Administration (FDA) specifically for the treatment of thrombocytopenia. (3) Results: The Hybrid CNN+DNN (HCD) model demonstrated the most effective predictive performance on the test dataset, achieving an accuracy of 98.3% and a precision of 97.0%. Both metrics surpassed the performance of the other models, and the model predicted that seven FDA drugs would exhibit activity. Isochlorogenic acid A, identified through screening the Chinese Pharmacopoeia Natural Product Library, was subsequently subjected to experimental verification. The results indicated a substantial enhancement in the differentiation and maturation of megakaryocytes (MKs), along with a notable increase in platelet production. (4) Conclusions: This underscores the potential therapeutic efficacy of isochlorogenic acid A in addressing RIT.

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Section: Discussionmentioning

confidence: 99%

An Innovative Inducer of Platelet Production, Isochlorogenic Acid A, Is Uncovered through the Application of Deep Neural Networks

Yi,

Luo,

Liao

et al. 2024

Biomolecules

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“…Although lung megakaryocytes produce functional platelets in mice, they also express MHC class II molecules and are enriched in immunologically relevant transcripts such as those encoding Toll-like receptors and chemokines 193 , which suggests that they have a role in immunosurveillance. Single-cell RNA sequencing identified three functionally distinct populations of megakaryocytes in mice and humans, specialized in platelet production, niche interaction or the inflammatory response 195 , 196 . Furthermore, these distinct megakaryocyte populations may produce platelets with different immunological attributes.…”

Section: Introductionmentioning

confidence: 99%

The role of platelets in immune-mediated inflammatory diseases

et al. 2023

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Immune-mediated inflammatory diseases (IMIDs) are characterized by excessive and uncontrolled inflammation and thrombosis, both of which are responsible for organ damage, morbidity and death. Platelets have long been known for their role in primary haemostasis, but they are now also considered to be components of the immune system and to have a central role in the pathogenesis of IMIDs. In patients with IMIDs, platelets are activated by disease-specific factors, and their activation often reflects disease activity. Here we summarize the evidence showing that activated platelets have an active role in the pathogenesis and the progression of IMIDs. Activated platelets produce soluble factors and directly interact with immune cells, thereby promoting an inflammatory phenotype. Furthermore, platelets participate in tissue injury and promote abnormal tissue healing, leading to fibrosis. Targeting platelet activation and targeting the interaction of platelets with the immune system are novel and promising therapeutic strategies in IMIDs.

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“…Peripheral blood samples were the most easily accessible biological specimens for clinical investigations on OHSS, and the blood collection technique was less hazardous to the patients [33] . Ustinova et al used metformin to sequence the peripheral blood transcriptome in a population, resulting in excellent sequencing data and functional enrichment [34] .…”

Section: Discussionmentioning

confidence: 99%

Peripheral blood transcriptome analysis of patients with ovarian hyperstimulation syndrome through high-throughput sequencing

Yan

Wang

et al. 2023

Reproductive and Developmental Medicine

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Objective: Ovarian hyperstimulation syndrome (OHSS) is a frequent iatrogenic complication that arises during assisted reproduction and accounts for approximately 30% of all in vitro fertilization cycles. Using high-throughput sequencing, we investigated the peripheral blood transcriptome of patients with OHSS.Methods: Peripheral blood samples were obtained from 15 patients in each of the OHSS high-risk and low-risk groups on the ovum pick-up day. Subsequently, high-throughput sequencing was used to obtain the peripheral blood transcriptomes of five patients each from the high-and low-risk groups. Bioinformatic tools were used for mRNA expression profile mapping and screening of differentially expressed genes (DEGs). Bioinformatics techniques were also implemented in the Kyoto Encyclopedia of Genes Genomes (KEGG) signal pathway, Gene Ontology (GO) function, and protein-protein interaction (PPI) network analyses of DEGs.Results: A total of 20,031 genes were identified and 148 were found to be differentially expressed (P <0.05, |log 2 FC| > 0.58), with 52 upregulated and 96 downregulated genes. GO and KEGG analyses indicated that these genes were involved in extracellular corpuscles (GO: 0070062), plasma membrane (GO: 0005886), extracellular regions (GO: 0005576), immune system response (GO: 0006955), PI3K-Akt signaling pathways (hsa04151), cell adhesion molecules (CAMs, hsa04514), focal adhesion (hsa04510), and complement and coagulation cascades (hsa04610). The PPI network and realtime fluorescence quantitative polymerase chain reaction (qPCR) verification predicted that complement C3, von Willebrand factor, and vascular cell adhesion protein 1 proteins are highly implicated in OHSS and may serve as potential biomarkers for future OHSS studies.Conclusion: Transcriptome analysis revealed several DEGs related to OHSS risk factors in the peripheral blood, indicating that these DEGs may be novel players in OHSS development.

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Global characterization of megakaryocytes in bone marrow, peripheral blood, and cord blood by single-cell RNA sequencing

Cited by 9 publications

References 53 publications

An Innovative Inducer of Platelet Production, Isochlorogenic Acid A, Is Uncovered through the Application of Deep Neural Networks

An Innovative Inducer of Platelet Production, Isochlorogenic Acid A, Is Uncovered through the Application of Deep Neural Networks

The role of platelets in immune-mediated inflammatory diseases

Peripheral blood transcriptome analysis of patients with ovarian hyperstimulation syndrome through high-throughput sequencing

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