Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent <i>Metschnikowia fructicola</i>

Hershkovitz, Vera; Ben-Dayan, C.; Raphael, Ginat; Pasmanik‐Chor, Metsada; Liu, Jia; Belausov, Eduard; Aly, Radi; Wisniewski, Michael; Droby, Samir

doi:10.1111/j.1364-3703.2011.00750.x

Cited by 73 publications

(58 citation statements)

References 48 publications

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“…The antagonistic yeasts may serve as an elicitor, triggering ROS signaling in host tissue and leading to the activation of host defenses (52)(53)(54). This premise is supported by studies analyzing gene/protein expression during yeast-fruit interactions (20,55,56). A proteomic analysis indicated that an antagonistic yeast, P. membranaefaciens, induced six antioxidant proteins in peach fruit, including catalase, glutathione peroxidase, and peroxiredoxin.…”

Section: Environmental Stressesmentioning

confidence: 94%

Responses of Yeast Biocontrol Agents to Environmental Stress

Yuan

Wisniewski

Droby

et al. 2015

Appl Environ Microbiol

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118

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c Biological control of postharvest diseases, utilizing wild species and strains of antagonistic yeast species, is a research topic that has received considerable attention in the literature over the past 30 years. In principle, it represents a promising alternative to chemical fungicides for the management of postharvest decay of fruits, vegetables, and grains. A yeast-based biocontrol system is composed of a tritrophic interaction between a host (commodity), a pathogen, and a yeast species, all of which are affected by environmental factors such as temperature, pH, and UV light as well as osmotic and oxidative stresses. Additionally, during the production process, biocontrol agents encounter various severe abiotic stresses that also impact their viability. Therefore, understanding the ecological fitness of the potential yeast biocontrol agents and developing strategies to enhance their stress tolerance are essential to their efficacy and commercial application. The current review provides an overview of the responses of antagonistic yeast species to various environmental stresses, the methods that can be used to improve stress tolerance and efficacy, and the related mechanisms associated with improved stress tolerance.

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Section: Environmental Stressesmentioning

confidence: 94%

Responses of Yeast Biocontrol Agents to Environmental Stress

Yuan

Wisniewski

Droby

et al. 2015

Appl Environ Microbiol

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118

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“…Previous transcriptome studies of Citrus fruit peel and peel sections were designed to investigate stress and defense responses but did not provide direct insights into essential oil biosynthesis (Maul et al, 2008;González-Candelas et al, 2010;Matas et al, 2010;Ballester et al, 2011;Hershkovitz et al, 2012). We optimized a protocol for the collection of intact ECs from the flavedo layer of grapefruit peel by laser microdissection and pressure catapulting, subsequent isolation of RNA, and linear amplification of transcripts for analysis on Affymetrix Citrus genome oligonucleotide microarrays.…”

Section: Analysis Of Global Transcript Expression Patterns In Ecs Surmentioning

confidence: 99%

“…For example, transcripts related to terpenoid synthases, which catalyze the first committed step in the biosynthesis of terpenoid essential oil constituents, were highly expressed in Citrus fruit peel when compared with other tissues and organs Dornelas and Mazzafera, 2007;Takita et al, 2007). More recently, genome-wide oligonucleotide microarray analyses have been conducted with Citrus peel tissue (Maul et al, 2008;González-Candelas et al, 2010;Matas et al, 2010;Ballester et al, 2011;Hershkovitz et al, 2012). However, these studies were aimed at evaluating specific developmental processes or stress/ defense responses of Citrus and did not provide direct insights into peel essential oil biosynthesis.…”

mentioning

confidence: 99%

Assessing the Biosynthetic Capabilities of Secretory Glands inCitrusPeel

2012

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Epithelial cells (ECs) lining the secretory cavities of Citrus peel have been hypothesized to be responsible for the synthesis of essential oil, but direct evidence for such a role is currently sparse. We used laser-capture microdissection and pressure catapulting to isolate ECs and parenchyma cells (as controls not synthesizing oil) from the peel of young grapefruit (Citrus × paradisi ‘Duncan’), isolated RNA, and evaluated transcript patterns based on oligonucleotide microarrays. A Gene Ontology analysis of these data sets indicated an enrichment of genes involved in the biosynthesis of volatile terpenoids and nonvolatile phenylpropanoids in ECs (when compared with parenchyma cells), thus indicating a significant metabolic specialization in this cell type. The gene expression patterns in ECs were consistent with the accumulation of the major essential oil constituents (monoterpenes, prenylated coumarins, and polymethoxylated flavonoids). Morphometric analyses demonstrated that secretory cavities are formed early during fruit development, whereas the expansion of cavities, and thus oil accumulation, correlates with later stages of fruit expansion. Our studies have laid the methodological and experimental groundwork for a vastly improved knowledge of the as yet poorly understood processes controlling essential oil biosynthesis in Citrus peel.

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“…Developments in deep sequencing, transcriptomics, MS-MS proteomics, metagenomics, comparative and functional genomics can be utilized to determine changes in the physiological status of biocontrol agents, and the effect of environmental stress on its intracellular machinery (Hershkovitz et al, 2011;Hershkovitz et al, 2013;Sui et al, 2015). Changes in the level of expression of "biocontrol genes" during mass production, formulation and storage, or in response to exposure and contact with host plant tissue after application can now be more readily investigated.…”

Section: Mechanisms Of Action Involved In Biocontrol Systemsmentioning

confidence: 99%

The science, development, and commercialization of postharvest biocontrol products

Droby

Wisniewski

Teixidó

et al. 2016

Postharvest Biology and Technology

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284

149

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A B S T R A C TPostharvest biological control agents as a viable alternative to the use of synthetic chemicals have been the focus of considerable research for the last 30 years by many scientists and several commercial companies worldwide. Several antagonists of postharvest pathogens have been identified and tested in laboratory, semi-commercial, and commercial settings and were developed into commercial products. The discovery and development of these antagonists into a product followed a paradigm in which a single antagonist isolated from one commodity was also expected to be effective on other commodities that vary in their genetic background, physiology, postharvest handling, and susceptibility to pathogens. In most cases, product development was successfully achieved but their full commercial potential was not realized. The low success rate of postharvest biocontrol products has been attributed to several problems, including difficulties in mass production and formulation of the antagonist, the physiological status of the harvested commodity and its susceptibility to specific pathogens. All these factors played a major role in the reduced and inconsistent performance of the biocontrol product when used under commercial conditions. Although many studies have been conducted on the mode of action of postharvest microbial antagonists, our understanding is still very incomplete. In this regard, a systems approach, that takes into account all the components of the biocontrol system, may represent the best approach to investigating the network of interactions that exist. Very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types, their function, the factors that influence the composition of the microbiota after harvest and during storage, and the distribution of individual taxa. In light of the progress made in recent years in metagenomic technologies, this technology should be used to characterize the composition of microbial communities on fruit and vegetables. Information on the dynamics and diversity of microbiota may be useful to developing a new paradigm in postharvest biocontrol that is based on constructing synthetic microbial communities that provide superior control of pathogens.

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Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent Metschnikowia fructicola

Cited by 73 publications

References 48 publications

Responses of Yeast Biocontrol Agents to Environmental Stress

Responses of Yeast Biocontrol Agents to Environmental Stress

Assessing the Biosynthetic Capabilities of Secretory Glands inCitrusPeel

The science, development, and commercialization of postharvest biocontrol products

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