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Ikeda, Takaaki; Noguchi, Osamu; Kobayashi, Fuminori; Tozuka, Shinichi; Tokushima, Kiko; Sakamoto, Shigemi; Marumo, Fumiaki; Sato, Chifumi

doi:10.1023/a:1018850805536

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…In principle, this could represent repair or turnover, but typically incorporation is allowed to occur for 18 h, more than enough time for all cells to pass through one cell cycle with DNA synthesis in S phase, and DNA replication is assumed to dominate the incorporation. Typically, the synthetic nucleoside bromodeoxyuridine (BrDU) [20,21] …”

Section: The Ltt Methods In Generalmentioning

confidence: 99%

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

Klein¹,

Schwenk²,

Heinrich‐Ramm³

et al. 2004

Pure and Applied Chemistry

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The lymphocyte transformation test (LTT) has been proven useful especially in the diagnosis of drug-induced allergic disorders. It is an in vitro test which is based on the fact that lymphocytes, which have been sensitized by a certain antigen, transform into blasts and proliferate when they are again exposed to this antigen. This proliferation is determined by measurement of the incorporation of [3H ]-thymidine or bromodeoxyuridine into replicating DNA. The test has the advantage over skin tests of avoiding re-exposure of individuals, and it was, therefore, hoped that it may also help to diagnose metal allergies and especially sensitization toward beryllium. However, the LTT measures only the sensitization of lymphocytes, but not the effector reaction, i.e., there may be positive results in exposed individuals even in the absence of clinical symptoms. There are several reports evaluating the LTT toward gold salts (Au), amalgam (Hg), nickel (Ni), beryllium (Be), and several other metals. With metals other than Be, the LTT appears to be of little use. In contrast, the LTT with Be may, indeed, define patients at risk of developing chronic beryllium disease (CBD), which affects mainly the respiratory tract and may even cause death. Beryllium sensitization progresses to CBD at a rate of 7-11 % per year. Since the Be-LTT can detect sensitization in workers who have not yet developed a disease it is an important diagnostic tool to detect individuals at risk. In conclusion, the LTT can detect a cell-mediated immunological response of an individual to metals. However, for most metals its usefulness is questionable, with the exception of Be; a positive Be-LTT can identify not only patients with CBD, but also persons at risk of developing CBD in later years.

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Section: The Ltt Methods In Generalmentioning

confidence: 99%

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

Klein¹,

Schwenk²,

Heinrich‐Ramm³

et al. 2004

Pure and Applied Chemistry

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“…Only nine out of 29 studies included in the analysis were dedicated exclusively to SCARs, namely to DRESS [ 16 , 17 , 18 , 19 ], SJS/TEN [ 20 , 21 ], or both SJS/TEN and DRESS [ 22 , 23 , 24 ]. The other 20 studies were conducted on patients with different drug hypersensitivity reactions (DHRs).…”

Section: Resultsmentioning

confidence: 99%

“…An additional 29 references were identified from the bibliographies of the retrieved articles. In total, 29 articles from both groups met the eligibility criteria and were reviewed and evaluated in detail ( Figure 1 ) [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 42 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

In Vitro Assays in Severe Cutaneous Adverse Drug Reactions: Are They Still Research Tools or Diagnostic Tests Already?

Porębski

2017

IJMS

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Severe cutaneous adverse drug reactions (SCARs) represent life-threatening medical conditions and an appropriate causative diagnosis of these conditions is of the highest importance. Existing in vivo diagnostic methods are risky or are just contraindicated in these patients. Therefore, in vitro tests take on greater significance. In this survey, the studies on in vitro assays in SCARs were identified with a defined searching strategy and strict eligibility criteria. Different methods in the particular clinical manifestations and the groups of drugs were compared in respect to the diagnostic parameters obtained. The lymphocyte transformation test and IFNg-ELISpot (Interferon γ-Enzyme-linked immunospot assay) appeared to have the best evidence currently available. Further diagnostic assays, which are based mostly on distinct mechanisms of SCARs, may outdo previous assays but they still need confirmation in a larger group of patients and in more research centers. Data from pediatric populations and acute generalized exanthematous pustulosis (AGEP) patients are scarce. Some technical issues, limitations, and modifications of routine laboratory methods are also discussed.

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Diagnostische Relevanz des Lymphozytentransformationstestes in der Umweltmedizin

2002

Bundesgesundheitsbl - Gesundheitsforsch - Gesundheitsschutz

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Untitled

Cited by 6 publications

References 23 publications

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

Diagnostic relevance of the lymphocyte transformation test for sensitization to beryllium and other metals (IUPAC Technical Report)

In Vitro Assays in Severe Cutaneous Adverse Drug Reactions: Are They Still Research Tools or Diagnostic Tests Already?

Diagnostische Relevanz des Lymphozytentransformationstestes in der Umweltmedizin

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