Gliotransmission of D-serine promotes thirst-directed behaviors in<i>Drosophila</i>

Park, Annie; Croset, Vincent; Otto, Nils; Agarwal, Devika; Treiber, Christoph Daniel; Meschi, Eleanora; Sims, David; Waddell, Scott

doi:10.1101/2022.03.07.483255

Cited by 4 publications

(6 citation statements)

References 178 publications

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“…Due to the heterogeneity in the size and shape of glial cells, adult fly brain cells were suspended into single cells and sorted by the criteria whether the cells express GFP (Figure 1B). Interestingly, the sorting plot revealed that 8-10% of total cells were GFP-positive, correlating with the reported number of glia in adult fly brains (Croset et al, 2018;Brunet Avalos et al, 2019;Park et al, 2022). After removal of cell debris, single GFP-positive glial cells were visualized by confocal microscopy (Figure 1C).…”

Section: Purification and Rna-sequencing Analysis Of Adult Fly Glia E...supporting

confidence: 65%

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Profiling neurotransmitter-evoked glial responses by RNA-sequencing analysis

Wang,

2023

Front. Neural Circuits

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Fundamental properties of neurons and glia are distinctively different. Neurons are excitable cells that transmit information, whereas glia have long been considered as passive bystanders. Recently, the concept of tripartite synapse is proposed that glia are structurally and functionally incorporated into the synapse, the basic unit of information processing in the brains. It has then become intriguing how glia actively communicate with the presynaptic and postsynaptic compartments to influence the signal transmission. Here we present a thorough analysis at the transcriptional level on how glia respond to different types of neurotransmitters. Adult fly glia were purified from brains incubated with different types of neurotransmitters ex vivo. Subsequent RNA-sequencing analyses reveal distinct and overlapping patterns for these transcriptomes. Whereas Acetylcholine (ACh) and Glutamate (Glu) more vigorously activate glial gene expression, GABA retains its inhibitory effect. All neurotransmitters fail to trigger a significant change in the expression of their synthesis enzymes, yet Glu triggers increased expression of neurotransmitter receptors including its own and nAChRs. Expressions of transporters for GABA and Glutamate are under diverse controls from DA, GABA, and Glu, suggesting that the evoked intracellular pathways by these neurotransmitters are interconnected. Furthermore, changes in the expression of genes involved in calcium signaling also functionally predict the change in the glial activity. Finally, neurotransmitters also trigger a general metabolic suppression in glia except the DA, which upregulates a number of genes involved in transporting nutrients and amino acids. Our findings fundamentally dissect the transcriptional change in glia facing neuronal challenges; these results provide insights on how glia and neurons crosstalk in a synaptic context and underlie the mechanism of brain function and behavior.

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Section: Purification and Rna-sequencing Analysis Of Adult Fly Glia E...supporting

confidence: 65%

“…F/F was calculated by fluorescence after addition of neurotransmitter subtracting the baseline fluorescence level (Tian et al, 2009;Vicario and Cali, 2019). To eliminate for baseline differences in F/F0 between samples, we normalized the "preconditioning" treatment for each cell to equal 0 (Park et al, 2022).…”

Section: Calcium Imagingmentioning

confidence: 99%

Profiling neurotransmitter-evoked glial responses by RNA-sequencing analysis

Wang,

2023

Front. Neural Circuits

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“…ATP can be released via hemichannels, exocytosis, anion channels, and lysosomes (Bezzi and Volterra, 2001;Fujii et al, 2017;Xiong et al, 2018). Lastly, D-serine has been shown to be released via exocytosis, BEST-1, and hemichannels (Wolosker et al, 1999;Martineau et al, 2013;Sild and Van Horn, 2013;Herman, 2018;Koh et al, 2022;Linsambarth et al, 2022;Park et al, 2022;Tapanes et al, 2022). Created with BioRender.com.…”

Section: Glutamatementioning

confidence: 99%

“…Glial D-serine is relevant for astrocytes across multiple species including Drosophila. In Drosophila, glial D-serine is required for thirst-directed behavior (Park et al, 2022). Many studies have shown that astrocytes can release D-serine under pathological conditions.…”

Section: D-serinementioning

confidence: 99%

Calcium signaling in astrocytes and gliotransmitter release

Goenaga

Araque

Kofuji

et al. 2023

Front. Synaptic Neurosci.

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Glia are as numerous in the brain as neurons and widely known to serve supportive roles such as structural scaffolding, extracellular ionic and neurotransmitter homeostasis, and metabolic support. However, over the past two decades, several lines of evidence indicate that astrocytes, which are a type of glia, play active roles in neural information processing. Astrocytes, although not electrically active, can exhibit a form of excitability by dynamic changes in intracellular calcium levels. They sense synaptic activity and release neuroactive substances, named gliotransmitters, that modulate neuronal activity and synaptic transmission in several brain areas, thus impacting animal behavior. This “dialogue” between astrocytes and neurons is embodied in the concept of the tripartite synapse that includes astrocytes as integral elements of synaptic function. Here, we review the recent work and discuss how astrocytes via calcium-mediated excitability modulate synaptic information processing at various spatial and time scales.

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“…Studies of peripheral wrapping glia uncovered intriguing links between glial-dependent clustering of voltage-gated ion channels and the evolution of myelination (Rey et al, 2023). Shaping higher-order brain functions, astrocytic D-serine gliotransmission promotes thirst-directed behaviors (Park et al, 2022).…”

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confidence: 99%

Editorial: Neurobiology of Drosophila: the 19th NeuroFly-2022 meeting

2023

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In September 2022, close to 375 neurobiologists from 35 different countries came together in Saint-Malo (France) to attend the 19th European Drosophila Neurobiology Conference (Figure 1). This biennial meeting is organized by scientists and takes place in different European cities, each time offering a distinct thematic flavor and personal touch. The strong participation highlighted both the vitality of the Drosophila neurobiology community and the need to meet colleagues in person to share our science and convivial moments.Sessions of NeuroFly-2022 were organized around the themes of Emerging Technologies, Brain Homeostasis and Metabolism, Brain Evolution and Ecology, Neural Development, Neural Circuits and Synapses, Brain Disorders, Sensory Systems and Behavior. Yet, sessions had no boundaries as thematic threads were taken up repeatedly. Assisted by web-based resources, such as "Virtual Fly Brain" described by Court et al., our knowledge of developing and adult neural circuits has become remarkably detailed. This, combined with cutting-edge genetic and other technologies, allowed the community to leap forward by addressing questions in an integrated manner-bridging connectomics and behavior, single cell transcriptomics and evolution of sensory systems, neuron and glial biology united in the context of nervous system development and function.Starting with the latest technological innovations, we learned that automated functional brain imaging now enables long-term recording of neural activity in the adult brain in situ to unravel the logic underlying locomotion patterns and awake/sleep cycles with single-cell resolution (Flores-Valle et al., 2022). Moreover, large-scale genomics approaches, including single-cell RNAseq transcriptomics, help to robustly assign molecular signatures to developing and adult neuronal subtypes, glia or hemocytes, and to embark on evolutionary comparisons (Konstantinides et al., 2022;Sakr et al., 2022).Throughout the meeting, presentations explored the mechanisms underlying learning and memory from different angles. Subcellular mechanisms controlling the transport and synaptic targeting of specific mRNAs shape long-term memory formation (Bauer et al., 2023). Brain homeostasis and metabolism studies highlighted the crucial control of energy flux in mushroom bodies by cortex glia for memory formation (Silva et al., 2022). Multisensory input results in improved memory retrieval through cross-modal binding of selective neurons via serotonergic microcircuits (Okray et al., 2023).

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Gliotransmission of D-serine promotes thirst-directed behaviors inDrosophila

Cited by 4 publications

References 178 publications

Profiling neurotransmitter-evoked glial responses by RNA-sequencing analysis

Profiling neurotransmitter-evoked glial responses by RNA-sequencing analysis

Calcium signaling in astrocytes and gliotransmitter release

Editorial: Neurobiology of Drosophila: the 19th NeuroFly-2022 meeting

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