Glioprotective Effect of Chitosan-Coated Rosmarinic Acid Nanoemulsions Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Rat Astrocyte Primary Cultures

Fachel, Flávia Nathiely Silveira; Prá, Morgana; Azambuja, Juliana H.; Endres, Marcelo; Bassani, Valquíria Linck; Koester, Letícia Scherer; Henriques, Amélia Teresinha; Barschak, Alethéa Gatto; Teixeira, Hélder Ferreira; Braganhol, Elizandra

doi:10.1007/s10571-019-00727-y

Cited by 27 publications

(13 citation statements)

References 54 publications

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“…Our laboratory revealed that PSME clearly showed in vitro antimutagenicity, and antioxidant and antiinflammatory activities [70]. RA, of which large quantities are found in various mint plants [71], including perilla [12,13,70], exhibits antioxidant, anti-inflammatory, antiallergic, and α-glucosidase-inhibitory activities [48,[72][73][74][75]. Moreover, RA can inhibit osteoclast differentiation [27] and promote osteoblastic cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of a Rosmarinic Acid-Enriched Fraction Prepared from Nga-Mon (Perilla frutescens) Seed Meal on Osteoclastogenesis through the RANK Signaling Pathway

et al. 2021

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The aim of this study is to determine antioxidant and anti-inflammatory activities relating to the antiosteoporosis effects of various perilla seed meal (PSM) fractions. The remaining waste of perilla seed obtained from cold oil compression was extracted with 70% ethanol and sequentially fractionated according to solvent polarity with hexane, dichloromethane, ethyl acetate, and water. The results indicated that the seed-meal ethyl acetate fraction (SMEF) exhibited the highest antioxidant and anti-inflammatory activities, and rosmarinic acid (RA) content. The signaling pathways induced by the receptor activator of the nuclear factor kappa B (NF-κB) ligand (RANKL) that trigger reactive oxygen species (ROS) and several transcription factors, leading to the induction of osteoclastogenesis, were also investigated. The SMEF clearly showed attenuated RANKL-induced tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts and TRAP activity. A Western blot analysis showed that the SMEF significantly downregulated RANKL-induced NF-κB, AP-1 activation, and the nuclear factor of activated T-cell 1 (NFATc1) expression. SMEF also suppressed RANKL-induced osteoclast-specific marker gene-like MMP-9 using zymography. Furthermore, the SMEF showed inhibition of RANKL-induced ROS production in RAW 264.7 cells. The results suggest that the SMEF, which contained high quantities of RA, could be developed as a natural active pharmaceutical ingredient for osteoclastogenic protection and health promotion.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of a Rosmarinic Acid-Enriched Fraction Prepared from Nga-Mon (Perilla frutescens) Seed Meal on Osteoclastogenesis through the RANK Signaling Pathway

et al. 2021

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“…However, in vivo studies are necessary to understand the neuroprotective potential of this delivery system. 107 …”

Section: Current Strategies To Improve the Treatment Of Alzheimer’s Diseasementioning

confidence: 99%

Improving Drug Delivery for Alzheimer’s Disease Through Nose-to-Brain Delivery Using Nanoemulsions, Nanostructured Lipid Carriers (NLC) and in situ Hydrogels

Cunha¹,

Forbes²,

Lobo³

et al. 2021

IJN

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Current treatments for Alzheimer’s disease (AD) attenuate the progression of symptoms and aim to improve the patient’s quality of life. Licensed medicines are mostly for oral administration and are limited by the difficulty in crossing the blood–brain barrier (BBB). Here in, the nasal route has been explored as an alternative pathway that allows drugs to be directly delivered to the brain via the nasal cavity. However, clearance mechanisms in the nasal cavity impair the delivery of drugs to the brain and limit their bioavailability. To optimize nose-to-brain delivery, formulations of lipid-based nanosystems, namely nanoemulsions and nanostructured lipid carriers (NLC), formulated in situ gelling hydrogels have been proposed as approaches for nose-to-brain delivery. These formulations possess characteristics that facilitate drug transport directly to the brain, minimizing side effects and maximizing therapeutic benefits. It has been recommended that the manufacture of these drug delivery systems follows the quality by design (QbD) approach based on nasal administration requirements. This review provides an insight into the current knowledge of the AD, highlighting the need for an effective drug delivery to the brain. Considering the mounting interest in the use of nanoemulsions and NLC for nose-to-brain delivery, a description of drug transport pathways in the nasal cavity and the application of these nanosystems and their in situ hydrogels through the intranasal route are presented. Relevant preclinical studies are summarised, and the future prospects for the use of lipid-based nanosystems in the treatment of AD are emphasized.

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“…Chitosan-coated rosmarinic acid nanoemulsions (RA CNE) have been shown to offer protection by inhibiting cellular death and repairing the astrocyte redox state in LPS-induced neuroinflammation and oxidative stress in astrocyte cells ( Fachel et al, 2020a ). Based on these in vitro results, researchers further illustrated the neuroprotective effects of RA CNE on the alleviation of neuroinflammation, oxidative stress, and memory deficit in Wistar rats ( Fachel et al, 2020b ).…”

Section: Organic Npsmentioning

confidence: 99%

“…specific-targeting and long-circulation time) can be maximized. In recent years, some researchers have reported the application of PEG-cationic bovine serum albumin ( Liu et al, 2013a ), PEG-PLA NPs ( Liu et al, 2013b ), PEG–PLGA NPs ( Zhang et al, 2014 ), chitosan-coated nanoemulsions ( Fachel et al, 2020a ), mSPAM ( Rajendrakumar et al, 2018 ), and CeNC/IONC/MSN-T807 ( Chen et al, 2018 ) as therapeutic strategies for neuroinflammation and neurodegenerative disease. Multifunctional NPs have extensive application prospects and warrant further exploration.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Nanoparticles: A Hope for the Treatment of Inflammation in CNS

Zhu

et al. 2021

Front. Pharmacol.

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Neuroinflammation, an inflammatory response within the central nervous system (CNS), is a main hallmark of common neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS), among others. The over-activated microglia release pro-inflammatory cytokines, which induces neuronal death and accelerates neurodegeneration. Therefore, inhibition of microglia over-activation and microglia-mediated neuroinflammation has been a promising strategy for the treatment of neurodegenerative diseases. Many drugs have shown promising therapeutic effects on microglia and inflammation. However, the blood–brain barrier (BBB)—a natural barrier preventing brain tissue from contact with harmful plasma components—seriously hinders drug delivery to the microglial cells in CNS. As an emerging useful therapeutic tool in CNS-related diseases, nanoparticles (NPs) have been widely applied in biomedical fields for use in diagnosis, biosensing and drug delivery. Recently, many NPs have been reported to be useful vehicles for anti-inflammatory drugs across the BBB to inhibit the over-activation of microglia and neuroinflammation. Therefore, NPs with good biodegradability and biocompatibility have the potential to be developed as an effective and minimally invasive carrier to help other drugs cross the BBB or as a therapeutic agent for the treatment of neuroinflammation-mediated neurodegenerative diseases. In this review, we summarized various nanoparticles applied in CNS, and their mechanisms and effects in the modulation of inflammation responses in neurodegenerative diseases, providing insights and suggestions for the use of NPs in the treatment of neuroinflammation-related neurodegenerative diseases.

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Glioprotective Effect of Chitosan-Coated Rosmarinic Acid Nanoemulsions Against Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Rat Astrocyte Primary Cultures

Cited by 27 publications

References 54 publications

Inhibitory Effect of a Rosmarinic Acid-Enriched Fraction Prepared from Nga-Mon (Perilla frutescens) Seed Meal on Osteoclastogenesis through the RANK Signaling Pathway

Inhibitory Effect of a Rosmarinic Acid-Enriched Fraction Prepared from Nga-Mon (Perilla frutescens) Seed Meal on Osteoclastogenesis through the RANK Signaling Pathway

Improving Drug Delivery for Alzheimer’s Disease Through Nose-to-Brain Delivery Using Nanoemulsions, Nanostructured Lipid Carriers (NLC) and in situ Hydrogels

Nanoparticles: A Hope for the Treatment of Inflammation in CNS

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