Glioneuronal apoptosis and neuroinflammation in drug resistant temporal lobe epilepsy

Sokolova, Tatyana V.; Литовченко, А. В.; Парамонова, Н. М.; Касумов, В. Р.; Kravtsova, Svetlana; Нездоровина, В. Г.; Sitovskaya, D. А.; Skiteva, E. N.; Бажанова, Е. Д.; Zabrodskaya, Yu. M.

doi:10.14412/2074-2711-2023-1-36-42

Cited by 3 publications

(3 citation statements)

References 25 publications

(25 reference statements)

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“…These changes include demyelination, rarefaction, and microcystic transformation of the neuropil, as well as neuronal devastation foci and atrophy of the temporal lobe cortex [13]. Reactive gliosis and neuroinflammation, which accompany neuronal damage, are also believed to contribute to the emergence of hyperexcitability foci [14]. Recent research has highlighted the critical role of glial cells (astrocytes, microglia, and oligodendrogliocytes) in various neurodegenerative diseases [15].…”

Section: Introductionmentioning

confidence: 99%

Expression of Cytoskeletal Proteins (GFAP, Vimentin), Proapoptotic Protein (Caspase-3) and Protective Protein (S100) in the Epileptic Focus in Adults and Children with Drug-Resistant Temporal Lobe Epilepsy Associated with Focal Cortical Dysplasia

Sitovskaya,

Zabrodskaya,

Parshakov

et al. 2023

IJMS

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The European Commission of the International League Against Epilepsy (ILAE) has identified glial mechanisms of seizures and epileptogenesis as top research priorities. The aim of our study was to conduct a comparative analysis of the expression levels of cytoskeletal proteins (glial fibrillar acidic protein (GFAP) and vimentin), protective protein S100, and proapoptotic caspase-3 protein in patients with drug-resistant epilepsy (DRE) associated with focal cortical dysplasia (FCD). We aimed to investigate how the expression levels of these proteins depend on age (both in children and adults), gender, and disease duration, using immunohistochemistry. Nonparametric statistical methods were employed for data analysis. In the epileptic focus area of the cortex and white matter in patients with FCD-associated temporal lobe DRE, a higher level of expression of these proteins was observed. Age and gender differences were found for vimentin and S100. In the early stages of disease development, there was a compensatory sequential increase in the expression of cytoskeletal and protective proteins. In patients with DRE, depending on the disease duration, patterns of development of neurodegeneration were noted, which is accompanied by apoptosis of gliocytes. These results provide insights into epilepsy mechanisms and may contribute to improving diagnostic and treatment approaches.

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Section: Introductionmentioning

confidence: 99%

Expression of Cytoskeletal Proteins (GFAP, Vimentin), Proapoptotic Protein (Caspase-3) and Protective Protein (S100) in the Epileptic Focus in Adults and Children with Drug-Resistant Temporal Lobe Epilepsy Associated with Focal Cortical Dysplasia

Sitovskaya,

Zabrodskaya,

Parshakov

et al. 2023

IJMS

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“…Ultrastructural changes in oligodendrogliocytes in DRE have been less studied; however, it has been shown that cells are most susceptible to apoptotic death [25], with the formation of Lewy bodies in the cytoplasm.…”

Section: Glia and Fi Bersmentioning

confidence: 99%

Ultrastructural Changes in Brain Tissue in Drug-Resistant Mesial Temporal Lobe Epilepsy in Humans: A Mini-Review

Sitovskaya,

Zabrodskaya

2023

J Biomed Res Environ Sci

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Epilepsy is a group of chronic neurological diseases of the brain characterized by a persistent predisposition to epileptic seizures, affecting more than 70 million people worldwide. Drug-resistance is a key problem in the theory and practice of epileptology. The literature contains many publications devoted to ultrastructural changes in brain tissue in experimental animal models, but the features of ultrastructural changes in human brain tissue in drug-resistant mesial Temporal Lobe Epilepsy (TLE) remain unknown. This brief review presents the current state of the art of problems devoted to the study of the ultrastructure of neurons, glia, and blood vessels in patients with drug-resistant mesial TLE.

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“…Increased expression of apoptotic markers, such as caspase-3, has been implicated in the pathogenesis of epilepsy. 3 Autophagy is another essential cellular process that involves the degradation and recycling of cellular components to maintain homeostasis. In epilepsy, dysregulation of autophagy has been proposed as a contributing factor to the neurodegenerative changes associated with the disorder.…”

Section: Introductionmentioning

confidence: 99%

Dysregulated Apoptosis and Autophagy in Childhood Epilepsy: Correlation to Clinical and Pharmacological Patterns

Ahmed,

Hassan,

Abdelfatah

et al. 2024

Neuropediatrics

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Objectives We aimed to assess the serum levels of caspase-3 as a marker of apoptosis and microtubule-associated protein 1A/1B-light chain 3 (MAP1-LC3) as an autophagy marker in epileptic children with various clinical and pharmacological types. Methods This case–control study was carried out on 90 participants (50 pediatric patients with epilepsy and 40 healthy matched children), the patients were categorized into three groups: Group (A): 25 pharmacosensitive epilepsy, Group (B): 25 pharmacoresistant epilepsy, and Group (C): 40 (age, sex, and body mass index) matched healthy children selected as controls. Serum caspase-3 and MAP1-LC3 were measured in all study groups, using commercially available ELISA kits. Results Serum caspase-3 was significantly higher among epileptic children, especially in the pharmacoresistant group, cases managed with multiple antiepileptic drugs, and cases with abnormal EEG findings. Conversely, circulating MAP1-LC3 levels showed a significant reduction in epilepsy cases, particularly in pharmacoresistant cases, in cases treated with multiple antiepileptic drugs, and in cases with abnormal EEG data. A significant negative correlation between serum caspase-3 and MAP1-LC3 was found among epileptic children (r = −0.369, p = 0.0083). Serum caspase-3 was a more valid biomarker in helping diagnose childhood epilepsy, while serum MAP1-LC3 was more valid in predicting pharmacoresistant type. Conclusion The study reveals that serum caspase-3 levels were significantly elevated, particularly in pharmacoresistant cases and those managed with multiple drugs. Conversely, MAP1-LC3 levels were significantly reduced in epilepsy cases, suggesting potential involvement of altered apoptosis and autophagy in childhood epilepsy.

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Glioneuronal apoptosis and neuroinflammation in drug resistant temporal lobe epilepsy

Cited by 3 publications

References 25 publications

Expression of Cytoskeletal Proteins (GFAP, Vimentin), Proapoptotic Protein (Caspase-3) and Protective Protein (S100) in the Epileptic Focus in Adults and Children with Drug-Resistant Temporal Lobe Epilepsy Associated with Focal Cortical Dysplasia

Expression of Cytoskeletal Proteins (GFAP, Vimentin), Proapoptotic Protein (Caspase-3) and Protective Protein (S100) in the Epileptic Focus in Adults and Children with Drug-Resistant Temporal Lobe Epilepsy Associated with Focal Cortical Dysplasia

Ultrastructural Changes in Brain Tissue in Drug-Resistant Mesial Temporal Lobe Epilepsy in Humans: A Mini-Review

Dysregulated Apoptosis and Autophagy in Childhood Epilepsy: Correlation to Clinical and Pharmacological Patterns

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