Glinus lotoides linn. Seed extract as antidiabetic agent: In vitro and in vivo anti-glucolipotoxicity efficacy in Type-II diabetes mellitus

Sisay, Woretaw; Andargie, Yared; Molla, Mulugeta; Desta, Getaye Tessema; Singh, Pradeep

doi:10.1016/j.metop.2022.100189

Cited by 2 publications

(4 citation statements)

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“…Elevated HDL aids in transportation of cholesterol to the liver, which is the primary site for fatty acid metabolism, thus reducing the risk of CVDs. So, in the diabetic condition, there is insulin deficiency due to which lipoproteins are unable to hydrolyze the lipids resulting in the systemic imbalance of synthesis, release, and rate of clearance of lipids ( Moodley et al, 2015 ) ( Sisay et al, 2022 ). In consistency with the reported data, we found elevated levels of TG and TC and decreased HDL cholesterol in the untreated STZ–NA-induced diabetic control group.…”

Section: Discussionmentioning

confidence: 99%

“…To examine the presence or absence of the major phytochemical group of compounds in the AAHY extract, such as alkaloids, phenols, flavonoids, saponins, tannins, glycosides, terpenoids, quinones, and steroids, we followed standard protocols for screening preliminary qualitative phytochemical profiling ( Banu and Cathrine, 2015 ; Sisay et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Glucose solution 20% was provided for the first 24 h to STZ–NA-injected rats to prevent initial hypoglycemic mortality. The diabetic condition was confirmed by fasting blood glucose (FBG) measurement of blood drawn from the tail vein using a glucometer (ACCU-Chek active), and FBG ≥250 mg/dL was selected for the experiment ( Ghasemi et al, 2014 ; Sisay et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…The normal control animals received normal saline water, and the treated groups were given predetermined doses of 200 and 400 mg/kg b.w., p.o., of the AAHY extract. The baseline blood glucose level of each rat was measured just prior to treatment (0 min) and after administration at 1, 2, 4, and 6 h, with the blood drawn from the tail vein under aseptic conditions ( Birru et al, 2015 ; Sisay et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

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Ageratina adenophora (Spreng.) King & H. Rob. Standardized leaf extract as an antidiabetic agent for type 2 diabetes: An in vitro and in vivo evaluation

et al. 2023

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Type 2 diabetes has become one of the major health concerns of the 21st century, marked by hyperglycemia or glycosuria, and is associated with the development of several secondary health complications. Due to the fact that chemically synthesized drugs lead to several inevitable side effects, new antidiabetic medications from plants have gained substantial attention. Thus, the current study aims to evaluate the antidiabetic capacity of the Ageratina adenophora hydroalcoholic (AAHY) extract in streptozotocin–nicotinamide (STZ–NA)-induced diabetic Wistar albino rats. The rats were segregated randomly into five groups with six rats each. Group I was normal control, and the other four groups were STZ–NA-induced. Group II was designated diabetic control, and group III, IV, and V received metformin (150 mg/kg b.w.) and AAHY extract (200 and 400 mg/kg b.w.) for 28 days. Fasting blood glucose, serum biochemicals, liver and kidney antioxidant parameters, and pancreatic histopathology were observed after the experimental design. The study concludes that the AAHY extract has a significant blood glucose lowering capacity on normoglycemic (87.01 ± 0.54 to 57.21 ± 0.31), diabetic (324 ± 2.94 to 93 ± 2.04), and oral glucose-loaded (117.75 ± 3.35 to 92.75 ± 2.09) Wistar albino rats. The in vitro studies show that the AAHY extract has α-glucosidase and α-amylase inhibitory activities which can restore the altered blood glucose level, glycated hemoglobin, body weight, and serum enzymes such as serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, total protein, urea, and creatinine levels close to the normal range in the treated STZ–NA-induced diabetic rats. The evaluation of these serum biochemicals is crucial for monitoring the diabetic condition. The AAHY extract has significantly enhanced tissue antioxidant parameters, such as superoxide dismutase, glutathione, and lipid peroxidation, close to normal levels. The presence of high-quantity chlorogenic (6.47% w/w) and caffeic (3.28% w/w) acids as some of the major phytoconstituents may contribute to the improvement of insulin resistance and oxidative stress. The study provides scientific support for the utilization of A. adenophora to treat type 2 diabetes in the STZ–NA-induced diabetic rat model. Although the preventive role of the AAHY extract in treating Wistar albino rat models against type 2 diabetes mellitus is undeniable, further elaborative research is required for efficacy and safety assessment in human beings.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%