Glicentin inhibits internalization of enteric bacteria by cultured INT-407 enterocytes

Abstract

Glicentin, the main component of enteroglucagon, has trophic effects on intestinal mucosa. It may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. We have established an in vitro bioassay system for determining the effects of recombinant human glicentin on bacterial internalization by confluent enterocytes. An INT-407 cell line was serum-deprived for 2 days and was then treated on transwell filters for 24 h with a medium containing one of the following: glicentin 100 ng-1 microg/ml, glucagons-like peptide-2 (GLP-2) 1 microg/ml, 10% fetal bovine serum (FCS), or without any growth factors. Pure cultures of Salmonella enteritidis, Escherichia coli, and Enterococcus faecalis were introduced to the upper chambers of the filter units. Following 2 h of incubation the numbers of bacteria in the lower chambers were measured. Pretreatment of enterocytes with glicentin inhibited bacterial internalization compared to untreated or GLP-2 enterocytes. Glicentin was associated with inhibition of enterocyte internalization of enteric bacteria by a mechanism that might be related to the integrity of the enterocyte adhesive junctions and tight junctions and to the production of sIgA. Glicentin seems to have a function as a barrier-sustaining agent that inhibits extraintestinal invasion of enteric bacteria.

Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability

“…GLP-2 is associated with intestinal growth and adaptation in a variety of pathological conditions, including post-resection intestinal adaptation, coeliac disease, parental nutrition-induced intestinal atrophy and inflammatory bowel disease 78 – 80. In addition, GLP-2 has also been demonstrated to enhance intestinal adaptation57 and to reduce the internalisation of enteric bacteria in INT-407 enterocytes in vitro model 81. Strikingly, the intestinotrophic effects observed following prebiotic administration are similar to the physiological effect of GLP-2.…”

Dipeptidyl Peptidase-4 (CD26): Knowing the Function before Inhibiting the Enzyme

“…(but with lower potency) with GLP-1 and glucagon receptors. Glicentin has trophic effects on intestinal mucosa and could have a function as a barrier-sustaining agent that inhibits extraintestinal invasion of enteric bacteria [19].…”

Classification and functions of enteroendocrine cells of the lower gastrointestinal tract

“…2002). Glicentin has been shown to exhibit a similar but less potent effect on intestinal epithelial cell proliferation and, together with GLP‐2, has been shown to be part of the adaptive response in short bowel syndrome, offering a promising therapeutic avenue in this condition as well as TPN‐associated gut atrophy (Drucker 2002; Chiba et al. 2007).…”

“…2007). Independent of its effect in epithelial cell proliferation, glicentin has also been implicated as a protective mucosal agent, preventing bacterial translocation of enteric bacteria when added to cell lines in vitro (Chiba et al. 2007).…”