Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia

Abstract

(1) Background: The bioavailability of nitric oxide (NO) and oxidative stress are important events related to the pathophysiology of preeclampsia (PE). In this present study, we aimed to evaluate the antioxidant effect of glibenclamide (GB) on the NO synthesis, oxidative stress, and antioxidant capacity in endothelial cells incubated with plasma from preeclamptic (PE) and normotensive pregnant women (NT). (2) Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with a plasma pool from 10 NT and 10 PE pregnant women; NO/NOx quantification and ROS levels were assessed by a fluorescence compound; lipid peroxidation was evaluated employing thiobarbituric acid (TBA); and total antioxidant capacity was measured by ferric reduction ability power (FRAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). (3) Results: We found that endothelial cells incubated with plasma from PE showed lower NO and NOx levels compared with the NT group. However, GB treatment increased these levels, as well as the antioxidant capacity. Furthermore, a decrease was observed in ROS generation and lipid peroxidation (4) Conclusions: The GB treatment exerted a positive effect on the NO/NOx production by HUVEC incubated with plasma from NT and PE pregnant women, as well as in the reduction in oxidative stress and increase in the antioxidant capacity.

Antioxidant and anti-inflammatory effects of Boswellia dalzielii and Hibiscus sabdariffa extracts in alloxan-induced diabetic rats

“…Thus, both extracts significantly reduce MDA levels while significantly increasing GSH, CAT, and SOD levels, similar to glibenclamide, as compared to the diabetic group of rats. Glibenclamide has been found to possess antioxidant effects [ 37 , 38 ]. The ability of our extracts to reduce the imbalance between ROS generation and enzymatic antioxidant activity in diabetic rats may explain the improvement in oxidative stress indicators.…”

Extracellular Vesicles Derived from Human Umbilical Cord MSC Improve Vascular Endothelial Function in In Vitro and In Vivo Models of Preeclampsia through Activating Arginine Metabolism

“…During the development of PE, a variety of endothelial cell dysfunctions can be observed, such as decreased proliferation, migration, and angiogenesis ability, cellular inflammatory response, oxidative stress, and vasoconstriction dysfunction . Researchers have found that relieving endothelial cell dysfunction through various means can treat PE, such as Priscila Rezeck Nunes using glyburide to enhance the antioxidant capacity of endothelial cell . Liu et al uses procyanidin B2 to attenuate sFlt-1-induced endothelial dysfunction and impaired angiogenesis, Gu et al uses esomeprazole to inhibit hypoxia/endothelial dysfunction-induced autophagy in PE .…”

“…20 Researchers have found that relieving endothelial cell dysfunction through various means can treat PE, such as Priscila Rezeck Nunes using glyburide to enhance the antioxidant capacity of endothelial cell. 21 Liu et al uses procyanidin B2 to attenuate sFlt-1-induced endothelial dysfunction and impaired angiogenesis, 22 Gu et al uses esomeprazole to inhibit hypoxia/endothelial dysfunctioninduced autophagy in PE. 23 Previous researchers have confirmed that endothelial cells are an effective target for the treatment of PE.…”

In Vitro Anti-Oxidant, In Vivo Anti-Hyperglycemic, and Untargeted Metabolomics-Aided-In Silico Screening of Macroalgae Lipophilic Extracts for Anti-Diabetes Mellitus and Anti-COVID-19 Potential Metabolites

“…The fasting blood glucose levels were assessed before induction and 72 days post alloxan induction using the Easy-Touch-GCU device (Bioptik Technology, Inc., Jhonghua, Taiwan). Only the rats showing fasting blood glucose levels exceeding 126 mg/dL were categorized as diabetic [55,56] and were included in the next phase of the study.…”