Glial fibrillary acidic protein immunoreactivity in the prefrontal cortex distinguishes younger from older adults in major depressive disorder

Miguel-Hidalgo, José Javier; Baucom, Christie C.; Dilley, Ginny E.; Overholser, James C.; Meltzer, Herbert Y.; Stockmeier, Craig A.; Rajkowska, Grażyna

doi:10.1016/s0006-3223(00)00999-9

Cited by 295 publications

(229 citation statements)

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“…The present study examines the level of GFAP expression in the dorsolateral prefrontal cortex in subjects with MDD and in age-matched control subjects. The purpose of this study is to test whether the relative levels of GFAP examined by Western blot analysis are in the same direction as changes in the distribution of GFAP immunoreactive structures determined by cell morphometric methods (Miguel-Hidalgo et al, 2000). Moreover, the present study further assesses age-related changes in GFAP protein levels in the dorsolateral prefrontal cortex suggested by our previous studies.…”

Section: Introductionmentioning

confidence: 81%

“…However, in this study, neither the laminar distribution of GFAP nor the number of GFAP immunoreactive astrocytes was analyzed. In contrast, in another morphometric study, GFAP immunoreactive astrocytes were analyzed in individual cortical layers of dorsolateral prefrontal area 9 in MDD (Miguel-Hidalgo et al, 2000). Although the packing density of astrocytes and the fraction of tissue covered by GFAP immunoreactive processes did not differ between depressed and control groups, there was a significant correlation between age and GFAP immunoreactivity among subjects with MDD.…”

Section: Introductionmentioning

confidence: 87%

“…Informed written consent was collected from the legal next-of-kin of all subjects. Next-of-kin were interviewed and retrospective psychiatric assessments were conducted in accordance with Institutional Review Board policies, as previously described (Miguel-Hidalgo et al, 2000;Rajkowska et al, 1999). A total of 15 subjects were diagnosed with MDD according to the Diagnostic and Statistic Manual of Mental DisordersRevised (DSM-III-R).…”

Section: Human Subjectsmentioning

confidence: 99%

“…Alterations in the density of immunoreactive astrocytes and the expression of the astrocytic marker glial fibrillary acidic protein (GFAP) have been identified in frontal cortex of subjects with MDD (Damadzic et al, 2001;Davis et al, 2002;Johnston-Wilson et al, 2000;Knable et al, 2001;Miguel-Hidalgo et al, 2000;Webster et al, 2001). A proteomic study in prefrontal area 10 revealed that the levels of four different isoforms of GFAP protein were significantly decreased in MDD (Johnston-Wilson et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Age-Dependent Reductions in the Level of Glial Fibrillary Acidic Protein in the Prefrontal Cortex in Major Depression

Miguel-Hidalgo

O'Dwyer

et al. 2004

Neuropsychopharmacol

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The density of glial cells is reduced in certain layers of the dorsolateral prefrontal cortex in major depressive disorder (MDD). Moreover, there are reductions in the packing density of glial fibrillary acidic protein (GFAP) immunoreactive astrocytes in the same cortical layers in younger subjects with MDD. The objective of the present study was to test if the level of GFAP is preferentially decreased in younger subjects with MDD, and whether GFAP levels are correlated with the age of onset of depression. Post-mortem brain tissue punches from dorsolateral prefrontal cortex were collected from 15 subjects with MDD and 15 age-matched psychiatrically normal control subjects. Western blots were performed on gels containing duplicated samples from both subjects of each matched pair, and on gels containing samples at different ages from either the MDD or the control group. The GFAP level was calculated as the ratio of the optical density of GFAP bands to actin bands in subjects with MDD and nonpsychiatric controls. Levels of GFAP were significantly lower in subjects with MDD as compared to controls and this decrease was most prominent in subjects less than 60 years old at the time of death. In the MDD group, GFAP levels were positively correlated with age at the time of death and show a trend toward correlation with the age of onset of depression. These findings indicate that a decrease in levels of GFAP may contribute to the pathophysiology of MDD, particularly in subjects of relatively young age.

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Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 87%

Section: Human Subjectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age-Dependent Reductions in the Level of Glial Fibrillary Acidic Protein in the Prefrontal Cortex in Major Depression

Miguel-Hidalgo

O'Dwyer

et al. 2004

Neuropsychopharmacol

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227

125

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“…(2004) who reported an increase in the density of glial cells in the hippocampus of major depression patients. Our result is also compatible with another postmortem study, which showed that older subjects with late‐onset depression had an increased astrocytic population in the dorsolateral prefrontal cortex (Miguel‐Hidalgo et al., 2000). On the other hand, several postmortem studies on major depression have given opposite findings, namely, prominent decrease in the number and the density of astrocytes in major depression patients compared to age‐matched nonpsychiatric controls.…”

Section: Discussionmentioning

confidence: 99%

Gunn rats with glial activation in the hippocampus show prolonged immobility time in the forced swimming test and tail suspension test

et al. 2018

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IntroductionRecent studies imply that glial activation plays a role in the pathogenesis of psychiatric disorders, such as schizophrenia and major depression. We previously demonstrated that Gunn rats with hyperbilirubinemia show congenital gliosis and schizophrenia‐like behavior.MethodsAs it has been suggested that major depression involves glial activation associated with neuroinflammation, we examined whether Gunn rats show depression‐like behavior using the forced swimming test (FST) and the tail suspension test (TST). In addition, we quantitatively evaluated both microgliosis and astrogliosis in the hippocampus of Gunn rats using immunohistochemistry analysis of the microglial marker ionized calcium‐binding adaptor molecule (Iba) 1 and the astrocytic marker S100B.ResultsBoth the FST and TST showed that immobility time of Gunn rats was significantly longer than that of normal control Wistar rats, indicating that Gunn rats are somewhat helpless, a sign of depression‐like behavior. In the quantification of immunohistochemical analysis, Iba1immunoreactivity in the dentate gyrus (DG), cornu ammonis (CA) 1, and CA3 and the number of Iba1‐positive cells in the CA1 and CA3 were significantly increased in Gunn rats compared to Wistar rats. S100B immunoreactivity in the DG, CA1, and CA3 and the number of S100B‐positive cells in the DG and CA3 were significantly increased in Gunn rats compared to Wistar rats.ConclusionOur findings suggest that both microglia and astrocyte are activated in Gunn rats and their learned helplessness could be related to glial activation.

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Subtype-Specific Alterations of γ-Aminobutyric Acid and Glutamatein Patients With Major Depression

Sanacora¹,

Gueorguieva²,

Epperson³

et al. 2004

Arch Gen Psychiatry

707

464

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The study replicates the findings of decreased GABA concentrations in the occipital cortex of subjects with MDD. It also demonstrates that there is a change in the ratio of excitatory-inhibitory neurotransmitter levels in the cortex of depressed subjects that may be related to altered brain function. Last, the combined data set suggests that magnetic resonance spectroscopy GABA measures may serve as a biological marker for a subtype of MDD.

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Glial fibrillary acidic protein immunoreactivity in the prefrontal cortex distinguishes younger from older adults in major depressive disorder

Cited by 295 publications

References 50 publications

Age-Dependent Reductions in the Level of Glial Fibrillary Acidic Protein in the Prefrontal Cortex in Major Depression

Age-Dependent Reductions in the Level of Glial Fibrillary Acidic Protein in the Prefrontal Cortex in Major Depression

Gunn rats with glial activation in the hippocampus show prolonged immobility time in the forced swimming test and tail suspension test

Subtype-Specific Alterations of γ-Aminobutyric Acid and Glutamatein Patients With Major Depression

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