Glial contributions to neuronal health and disease: new insights from Drosophila

Logan, Mary A.

doi:10.1016/j.conb.2017.10.008

Cited by 18 publications

(18 citation statements)

References 52 publications

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“…7). It is known that Drosophila astrocytes (ALG) are signi cant contributors to modulate neurotransmitter homeostasis within synapses [18] and also regulators of circadian rhythm [19].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is likely that abolishing the functions of ALG or ALG-speci c genes could interfere with normal locomotion properties and lifespan of D. melanogaster, which are the direct outcome of dysregulations in neurotransmission and circadian rhythm, respectively. Moreover, it was recently reported that mitochondrial dysfunctions in ALG cells contribute to neurodegeneration [18,20], which could give rise to shortened lifespan of D. melanogaster [21].…”

Section: Discussionmentioning

confidence: 99%

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Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila

Najafi

Wong

Kim

2020

Preprint

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Background Drosophila melanogaster is a proper model organism for studying the development and function of the nervous system. The Drosophila nervous system consists of distinct cell types with significant homologies to various cell types of more advanced organisms, including human. Among all cell types of the nervous system, astrocyte-like glia (ALG) have conserved functions to mammals and are essential for normal physiology and behaviours of the fly. Results In this study, we exploited the gene expression profile of single cells in Drosophila optic lobe to identify the genes with specific expression pattern in each cell type. Through a bioinformatical analysis of the data, a novel ALG-specific gene (here assigned as deathstar ) was identified. Immunostaining of deathstar in the central nervous system (CNS) showed its presence in specific regions of Drosophila ventral nerve cord, which previously has been characterized as ALG cells. Consistent with the bioinformatical analysis, deathstar -related signals were overlapped with the signals of the previously-reported ALG marker, Eaat1 , supporting its specific expression in ALG cells. At the physiological level, RNAi-mediated suppression of deathstar gene impeded the normal development of male flies without any effects on females. Cell type-specific expression of deathstar RNAi showed that deathstar gene affects locomotion behaviour and lifespan of D. melanogaster , in an ALG-specific manner. Conclusions Taken together, we showed that bioinformatical analysis of a previously reported expression data of Drosophila optic lobe successfully predicted the ALG-specific expression pattern of deathstar gene. Moreover, it was consistent with the ALG-specific effects of this gene on locomotion and lifespan of D. melanogaster, in vivo .

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“…7). It is known that Drosophila astrocytes (ALG) are signi cant contributors to modulate neurotransmitter homeostasis within synapses [18] and also regulators of circadian rhythm [19].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila

Najafi

Wong

Kim

2020

Preprint

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show abstract

“…7). It is known that Drosophila astrocytes (ALG) are significant contributors to modulate neurotransmitter homeostasis within synapses [23] and also regulators of circadian rhythm [24].…”

Section: Discussionmentioning

confidence: 99%

The astrocyte-enriched geneCG11000plays a crucial role in the development, locomotion and lifespan ofD. melanogaster

Najafi¹,

Wong²,

Salkini³

et al. 2019

Preprint

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Drosophila melanogaster is a proper model organism for studying the development and function of the nervous system. The Drosophila nervous system consists of distinct cell types with significant homologies to various cell types of more advanced organisms, including human.Among all cell types of the nervous system, astrocyte-like glia (ALG) have conserved functions to mammals and are essential for normal physiology and behaviours of the fly.In this study, we exploited the gene expression profile of single cells in Drosophila optic lobe to identify the genes with specific expression pattern in each cell type. Through a bioinformatical analysis of the data, a novel ALG-specific gene (here assigned as deathstar) was identified.Immunostaining of deathstar in the central nervous system (CNS) showed its presence in specific regions of Drosophila ventral nerve cord, which previously has been characterized as ALG cells.Consistent with the bioinformatical analysis, deathstar-related signals were overlapped with the signals of the previously-reported ALG marker, Eaat1, supporting its specific expression in ALG cells.At the physiological level, RNAi-mediated suppression of deathstar gene impeded the normal development of male flies without any effects on females. Cell type-specific expression of deathstar RNAi showed that deathstar gene affects locomotion behaviour and lifespan of D. melanogaster, in an ALG-specific manner.Taken together, we showed that bioinformatical analysis of a previously reported expression data of Drosophila optic lobe successfully predicted the ALG-specific expression pattern of deathstar gene. Moreover, it was consistent with the ALG-specific effects of this gene on locomotion and lifespan of D. melanogaster, in vivo.

show abstract

“…7). It is known that Drosophila astrocytes (ALG) are significant contributors to modulate neurotransmitter homeostasis within synapses [18] and also regulators of circadian rhythm [19].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is likely that abolishing the functions of ALG or ALG-specific genes could interfere with normal locomotion properties and lifespan of D. melanogaster, which are the direct outcome of dysregulations in neurotransmission and circadian rhythm, respectively. Moreover, it was recently reported that mitochondrial dysfunctions in ALG cells contribute to neurodegeneration [18,20], which could give rise to shortened lifespan of D. melanogaster [21].…”

Section: Discussionmentioning

confidence: 99%

Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila

Najafi

Wong

Kim

2019

Preprint

View full text Add to dashboard Cite

Background: Drosophila melanogaster is a proper model organism for studying the development and function of the nervous system. The Drosophila nervous system consists of distinct cell types with significant homologies to various cell types of more advanced organisms, including human. Among all cell types of the nervous system, astrocyte-like glia (ALG) have conserved functions to mammals and are essential for normal physiology and behaviours of the fly.Results: In this study, we exploited the gene expression profile of single cells in Drosophila optic lobe to identify the genes with specific expression pattern in each cell type. Through a bioinformatical analysis of the data, a novel ALG-specific gene (here assigned as deathstar) was identified. Immunostaining of deathstar in the central nervous system (CNS) showed its presence in specific regions of Drosophila ventral nerve cord, which previously has been characterized as ALG cells. Consistent with the bioinformatical analysis, deathstar-related signals were overlapped with the signals of the previously-reported ALG marker, Eaat1, supporting its specific expression in ALG cells. At the physiological level, RNAi-mediated suppression of deathstar gene impeded the normal development of male flies without any effects on females. Cell type-specific expression of deathstar RNAi showed that deathstar gene affects locomotion behaviour and lifespan of D. melanogaster, in an ALG-specific manner.Conclusions: Taken together, we showed that bioinformatical analysis of a previously reported expression data of Drosophila optic lobe successfully predicted the ALG-specific expression pattern of deathstar gene. Moreover, it was consistent with the ALG-specific effects of this gene on locomotion and lifespan of D. melanogaster, in vivo.

show abstract

Glial contributions to neuronal health and disease: new insights from Drosophila

Cited by 18 publications

References 52 publications

Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila

Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila

The astrocyte-enriched geneCG11000plays a crucial role in the development, locomotion and lifespan ofD. melanogaster

Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila

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