Glial Cell Line-Derived Neurotrophic Factor Chitosan Nanolipid Carrier Inhibits Intestinal Carcinoma via Restraining NF-κB Activity

Abstract

Background: Glial cell line derived neurotrophic factor (GDNF) is a potent neurotrophic factor, which has been shown to affect the metastasis and invasion of cancer cells. The molecular mechanism of GDNF is still unclear. Here, we investigate the mechanism of GDNF embedded in chitosan (CS) nano lipid carrier in colorectal cancer. Methods: Electron microscopy was used to detect the relationship between the characteristics of GDNF chitosan (CS) coated nano lipid carrier, MTT and apoptosis tests were used to detect the effect of GDNF chitosan nano lipid carrier expression on the proliferation ability of intestinal cancer cells, and immunofluorescence was used to detect the effect of GDNF chitosan nano lipid carrier expression on NF-KB signal. The results were verified by western-blot. In vivo using a mouse model. Results: GDNF chitosan nano lipid carrier showed positive zeta value, indicating that the process of CS coating GDNF was successful. OD values of nanocomposites at different concentrations were measured by UV spectrophotometer with MTT. Our data showed that the experimental group showed a relationship between concentration and dose dependent on tolerance growth, indicating that GDNF chitosan nano lipid carrier had better anti-tumor activity in colorectal cancer cell lines. Compared with most GDNF chitosan nano lipid carrier groups, the expression of NF-κB immunofluorescence detection signal was inhibited, and the expression of NF-κB was down-regulated in colorectal cancer. In vivo results showed that the measured tumor volume decreased after treatment with GDNF chitosan nanoparticle lipid carrier, and the survival of mice treated with GDNF chitosan nanoparticle lipid carrier was longer. Conclusion: GDNF chitosan nano lipid carrier can inhibit the expression of NF-κB signal and reduce the proliferation of tumor In vivo, thus slowing down the occurrence and development of intestinal cancer cells.