Glia-related circadian plasticity in the visual system of Diptera

Górska-Andrzejak, Jolanta

doi:10.3389/fphys.2013.00036

Cited by 18 publications

(12 citation statements)

References 55 publications

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“…In the present study, we examined the pattern of DmMANF expression and its importance in neurons and glial cells of the Drosophila brain, especially in the first neuropil of the visual system (Figure 1 ). Interactions between neurons and glial cells in this neuropil display high plasticity, including circadian plasticity, which we have reported (Górska-Andrzejak et al, 2009 , 2013 ; Górska-Andrzejak, 2013 ; Woznicka et al, 2015 ). Additionally, the lamina is a convenient model for studying various processes in the nervous system due to its regular structure formed by cylindrical units called cartridges (Nériec and Desplan, 2016 ).…”

Section: Introductionsupporting

confidence: 54%

Downregulation of DmMANF in Glial Cells Results in Neurodegeneration and Affects Sleep and Lifespan in Drosophila melanogaster

et al. 2017

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In Drosophila melanogaster, mesencephalic astrocyte-derived neurotrophic factor (DmMANF) is an evolutionarily conserved ortholog of mammalian MANF and cerebral dopamine neurotrophic factor (CDNF), which have been shown to promote the survival of dopaminergic neurons in the brain. We observed especially high levels of DmMANF in the visual system of Drosophila, particularly in the first optic neuropil (lamina). In the lamina, DmMANF was found in glial cells (surface and epithelial glia), photoreceptors and interneurons. Interestingly, silencing of DmMANF in all neurons or specifically in photoreceptors or L2 interneurons had no impact on the structure of the visual system. However, downregulation of DmMANF in glial cells induced degeneration of the lamina. Remarkably, this degeneration in the form of holes and/or tightly packed membranes was observed only in the lamina epithelial glial cells. Those membranes seem to originate from the endoplasmic reticulum, which forms autophagosome membranes. Moreover, capitate projections, the epithelial glia invaginations into photoreceptor terminals that are involved in recycling of the photoreceptor neurotransmitter histamine, were less numerous after DmMANF silencing either in neurons or glial cells. The distribution of the alpha subunit of Na+/K+-ATPase protein in the lamina cell membranes was also changed. At the behavioral level, silencing of DmMANF either in neurons or glial cells affected the daily activity/sleep pattern, and flies showed less activity during the day but higher activity during the night than did controls. In the case of silencing in glia, the lifespan of flies was also shortened. The obtained results showed that DmMANF regulates many functions in the brain, particularly those dependent on glial cells.

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Section: Introductionsupporting

confidence: 54%

Downregulation of DmMANF in Glial Cells Results in Neurodegeneration and Affects Sleep and Lifespan in Drosophila melanogaster

et al. 2017

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“…Pigment glial cells of the eye ensheath photoreceptor neuron cell bodies while epithelial glia surround axon terminals (Edwards and Meinertzhagen, 2010). These glial cells in the Drosophila eye strongly express ATPα (Gorska-Andrzejak, 2013). It is not surprising then that overexpression of Dube3a specifically within glial cells using the repo- GAL4 driver line causes defects in the ERG signal, potentially through the downregulation of ATPα.…”

Section: Discussionmentioning

confidence: 99%

Glial overexpression of Dube3a causes seizures and synaptic impairments in Drosophila concomitant with down regulation of the Na+/K+ pump ATPα

Hope

LeDoux

Reiter

2017

Neurobiology of Disease

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Duplication 15q syndrome (Dup15q) is an autism-associated disorder co-incident with high rates of pediatric epilepsy. Additional copies of the E3 ubiquitin ligase UBE3A are thought to cause Dup15q phenotypes, yet models overexpressing UBE3A in neurons have not recapitulated the epilepsy phenotype. We show that Drosophila endogenously expresses Dube3a (fly UBE3A homolog) in glial cells and neurons, prompting an investigation into the consequences of glial Dube3a overexpression. Here we expand on previous work showing that the Na+/K+ pump ATPα is a direct ubiquitin ligase substrate of Dube3a. A robust seizure-like phenotype was observed in flies overexpressing Dube3a in glial cells, but not neurons. Glial-specific knockdown of ATPα also produced seizure-like behavior, and this phenotype was rescued by simultaneously overexpressing ATPα and Dube3a in glia. Our data provides the basis of a paradigm shift in Dup15q research given that clinical phenotypes have long been assumed to be due to neuronal UBE3A overexpression.

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“…The expression of clock and ccgs have not been studied in the visual system—a site of circadian plasticity and in glial cells that participate in the regulation of circadian rhythms in the brain and in behavior (Jackson, 2011 ; Górska-Andrzejak, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Clock and clock-controlled genes are differently expressed in the retina, lamina and in selected cells of the visual system of Drosophila melanogaster

Damulewicz

Łoboda

Bukowska-Straková

et al. 2015

Front. Cell. Neurosci.

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The retina and the first optic neuropil (lamina) of Drosophila show circadian rhythms in various processes. To learn about the regulation of circadian rhythms in the retina and lamina and in two cell types, glial and the lamina L2 interneurons, we examined expression of the following clock genes; per, tim, clk, and cry and clock-controlled genes (ccgs); Atpα, nrv2, brp, Pdfr. We found that the expression of gene studied is specific for the retina and lamina. The rhythms of per and tim expression in the retina and glial cells are similar to that observed in the whole head and in clock neurons, while they differ in the lamina and L2 cells. In both the retina and lamina, CRY seems to be a repressor of clk expression. In L2 interneurons per expression is not cyclic indicating the other function of PER in those cells than in the circadian molecular clock. In contrast to per and tim, the pattern of clk and cry expression is similar in both the retina and lamina. The retina holds the autonomous oscillators but the expression of cry and ccgs, Atpα and nrv2, is also regulated by inputs from the pacemaker transmitted by PDF and ITP neuropeptides.

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Glia-related circadian plasticity in the visual system of Diptera

Cited by 18 publications

References 55 publications

Downregulation of DmMANF in Glial Cells Results in Neurodegeneration and Affects Sleep and Lifespan in Drosophila melanogaster

Downregulation of DmMANF in Glial Cells Results in Neurodegeneration and Affects Sleep and Lifespan in Drosophila melanogaster

Glial overexpression of Dube3a causes seizures and synaptic impairments in Drosophila concomitant with down regulation of the Na+/K+ pump ATPα

Clock and clock-controlled genes are differently expressed in the retina, lamina and in selected cells of the visual system of Drosophila melanogaster

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