GLH-1, the<i>C. elegans</i>P granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5

Orsborn, April; Li, Wensheng; McEwen, Tamara J.; Mizuno, Tomoaki; Kuzmin, Evgeny V.; Matsumoto, Kunihiro; Bennett, Karen

doi:10.1242/dev.005181

Cited by 41 publications

(52 citation statements)

References 56 publications

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“…This is different from 24.5°C, where glh-1 mRNA and protein levels both are reduced 5-to 10-fold in the M+Z-generation. Several groups have noted that GLH-1 protein is more abundant at low temperatures than high temperatures (Orsborn et al, 2007;Walstrom et al, 2005) (data not shown), suggesting that GLH-1 is thermolabile. If GLH-1 is very stable at 15°C, perdurance of GLH-1 might obscure the effects of a reduction in glh-1 mRNA levels in the M+Z-generation.…”

Section: Developmentmentioning

confidence: 94%

DEPS-1 promotes P-granule assembly and RNA interference inC. elegansgerm cells

et al. 2008

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P granules are germ-cell-specific cytoplasmic structures containing RNA and protein, and required for proper germ cell development in C. elegans. PGL-1 and GLH-1 were previously identified as critical components of P granules. We have identified a new P-granule-associated protein, DEPS-1, the loss of which disrupts P-granule structure and function. DEPS-1 is required for the proper localization of PGL-1 to P granules, the accumulation of glh-1 mRNA and protein, and germ cell proliferation and fertility at elevated temperatures. In addition, DEPS-1 is required for RNA interference (RNAi) of germline-expressed genes, possibly because DEPS-1 promotes the accumulation of RDE-4, a dsRNA-binding protein required for RNAi. A genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1 regulation, many of which are also regulated by the RNAi factor RDE-3. Our studies suggest that DEPS-1 is a key component of the P-granule assembly pathway and that its roles include promoting accumulation of some mRNAs, such as glh-1 and rde-4, and reducing accumulation of other mRNAs, perhaps by collaborating with RDE-3 to generate endogenous short interfering RNAs (endo-siRNAs).

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Section: Developmentmentioning

confidence: 94%

DEPS-1 promotes P-granule assembly and RNA interference inC. elegansgerm cells

et al. 2008

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“…74 In contrast, proteasomal turnover of the helicase GLH-1 in C. elegans P-granules appears to be necessary to prevent excessive P-granule assembly that leads to sterility. 75 Factors associated with proteasomal function were also identified in screens for factors affecting P-granule, 76 P-body, and stress granule assembly. 77 Chaperone proteins also affect disassembly of stress granules.…”

Section: Mrnp Granules Assemble Via Common Mechanismsmentioning

confidence: 99%

mRNP granules

2014

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“…Based on conservation of TAO kinases, it is possible that KIN-18 also activates p38 (MAPK) or JNK (MAPK) signaling to activate those targets in C. elegans. Nevertheless, viable mutants of p38 orthologs in C. elegans do not affect the germline; two JNK orthologs interact with P-granule proteins, making it difficult to dissect their specific function in JNK signaling (Berman et al 2001a;Smith et al 2002;Orsborn et al 2007). Second, KIN-18 may function independently of its kinase activity by interacting with one of the proteins involved in the canonical MPK-1 cascade, preventing its activation.…”

Section: Mpk-1 Signaling and The Timing Of Meiotic Dsb Repair Eventsmentioning

confidence: 99%

Coordination of Recombination with Meiotic Progression in the Caenorhabditis elegans Germline by KIN-18, a TAO Kinase That Regulates the Timing of MPK-1 Signaling

Yin¹,

Donlevy²,

Smolikove

2015

Genetics

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Meiosis is a tightly regulated process requiring coordination of diverse events. A conserved ERK/MAPK-signaling cascade plays an essential role in the regulation of meiotic progression. The Thousand And One kinase (TAO) kinase is a MAPK kinase kinase, the meiotic role of which is unknown. We have analyzed the meiotic functions of KIN-18, the homolog of mammalian TAO kinases, in Caenorhabditis elegans. We found that KIN-18 is essential for normal meiotic progression; mutants exhibit accelerated meiotic recombination as detected both by analysis of recombination intermediates and by crossover outcome. In addition, ectopic germ-cell differentiation and enhanced levels of apoptosis were observed in kin-18 mutants. These defects correlate with ectopic activation of MPK-1 that includes premature, missing, and reoccurring MPK-1 activation. Late progression defects in kin-18 mutants are suppressed by inhibiting an upstream activator of MPK-1 signaling, KSR-2. However, the acceleration of recombination events observed in kin-18 mutants is largely MPK-1-independent. Our data suggest that KIN-18 coordinates meiotic progression by modulating the timing of MPK-1 activation and the progression of recombination events. The regulation of the timing of MPK-1 activation ensures the proper timing of apoptosis and is required for the formation of functional oocytes. Meiosis is a conserved process; thus, revealing that KIN-18 is a novel regulator of meiotic progression in C. elegans would help to elucidate TAO kinase's role in germline development in higher eukaryotes.KEYWORDS KIN-18; MPK-1; TAO; meiosis; recombination; genetics of sex T HE germline contains a multitude of cells that proliferate to form a population that will undergo two meiotic divisions, giving rise to gametes. In meiosis, one round of DNA replication is followed by two cell divisions reducing the ploidy of the germ cell by half. A large fraction of the cells encompassing the germline are found in meiotic prophase I. Many of the key evolutionarily conserved events that take place in this specialized prophase are aimed at preparing chromosomes for their segregation in meiosis I. One such essential and conserved event is the formation of crossovers between homologous chromosomes. During the initial stages of meiotic prophase I (leptotene/zygotene), a protein structure named the synaptonemal complex (SC) starts to assemble between homologs (MacQueen et al. 2002;Schild-Prufert et al. 2011). Concurrently, meiotic DNA double-strand breaks (DSBs) are introduced to initiate meiotic recombination (Keeney et al. 1997;Dernburg et al. 1998). As meiosis progresses, germ cells enter pachytene where the SC is fully formed and meiotic DSBs are processed and repaired. In the presence of a fully formed SC, meiotic DSBs are repaired, giving rise to crossovers by late pachytene. In most multicellular organisms, apoptosis is activated in late pachytene and reduces the number of germ cells in the developing germline (Gumienny et al. 1999). Apoptosis serves as a quality control...

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GLH-1, theC. elegansP granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5

Cited by 41 publications

References 56 publications

DEPS-1 promotes P-granule assembly and RNA interference inC. elegansgerm cells

DEPS-1 promotes P-granule assembly and RNA interference inC. elegansgerm cells

mRNP granules

Coordination of Recombination with Meiotic Progression in the Caenorhabditis elegans Germline by KIN-18, a TAO Kinase That Regulates the Timing of MPK-1 Signaling

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