Glaucomatous cell derived matrices differentially modulate non-glaucomatous trabecular meshwork cellular behavior

Raghunathan, Vijay Krishna; Benoit, Julia S.; Kasetti, Ramesh Babu; Zode, Gulab; Salemi, Michelle; Phinney, Brett S.; Keller, Kate E.; Staverosky, Julia A.; Murphy, Christopher J.; Acott, Ted S.; Vranka, Janice A.

doi:10.1016/j.actbio.2018.02.037

Cited by 64 publications

(61 citation statements)

References 91 publications

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“…This was not observed in human TM cells exposed to a control medium. In another study of the group of Raghunathan et al 42 the same results were reported. Li et al 43 found similar results after exposing the TM of mice to dexamethasone.…”

Section: Figuresupporting

confidence: 61%

The Molecular Processes in the Trabecular Meshwork After Exposure to Corticosteroids and in Corticosteroid-Induced Ocular Hypertension

Liesenborghs

Eijssen

Kutmon

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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To identify processes that contribute to corticosteroid-induced ocular hypertension and candidate target genes for treatment. METHODS. A systematic search identified five human microarray datasets investigating the effect of dexamethasone versus a control medium on trabecular meshwork (TM) tissue. After thorough quality control, samples of low quality were removed, and the datasets were integrated. Additionally, a bovine RNA-sequencing dataset allowed to investigate differences in gene expression profiling between cows with and without corticosteroidinduced ocular hypertension (responders vs. nonresponders). The obtained datasets were used as input for parallel pathway analyses. Significantly changed pathways were clustered into functional categories and the results were further investigated. A network visualizing the differences between the responders and nonresponders was created. RESULTS. Seven functional pathway clusters were found to be significantly changed in TM cells exposed to dexamethasone versus a control medium and in TM cells of responders versus nonresponders: collagen, extracellular matrix, adhesion, WNT-signaling, inflammation, adipogenesis, and glucose metabolism. In addition, cell cycle and senescence were only significantly changed in responders versus nonresponders. The network of the differential gene expression between responders and nonresponders shows many connections between the identified processes via shared genes. CONCLUSIONS. Nine functional pathway clusters synthesize the molecular response to dexamethasone exposure in TM cells and are likely to be involved in the pathogenesis of corticosteroid-induced ocular hypertension.

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Section: Figuresupporting

confidence: 61%

The Molecular Processes in the Trabecular Meshwork After Exposure to Corticosteroids and in Corticosteroid-Induced Ocular Hypertension

Liesenborghs

Eijssen

Kutmon

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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“…Importantly, dECM bioinks may be used as allogeneic or even xenogenic materials in tissue engineering applications, since their production and decellularization processes involve harsh enzymatic and detergent treatments, aimed at removing immunogenic residual deoxyribonucleic acid (DNA) or cell membrane antigen fragments. Previous research has also pointed out how ECM composition varies between degenerative and homeostatic processes, thus opening interesting avenues for using dECM bioinks from nonhealthy organs to create in vitro models to study tissue pathophysiology. The exact composition of ECM‐derived materials, particularly that of less abundant compounds, is not fully defined, although proteomic techniques can aid elucidating part of these components .…”

Section: Strategies To Evolve From Shape To Functionmentioning

confidence: 99%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

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In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

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“…The rigidity of the ECM, which is altered in POAG patients [35] and in glaucomatous cell cultures [117], is likely to affect the structure and activity of fibronectin fibrils. The type III repeats in fibronectin lack intrachain disulfide bonds which enables cell contraction to stretch these repeats and to expose cryptic sites with unique biological activities.…”

Section: Increased Ecm Rigiditymentioning

confidence: 99%

Role of Fibronectin in Primary Open Angle Glaucoma

Faralli

Filla

Peters

2019

Cells

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Primary open angle glaucoma (POAG) is the most common form of glaucoma and the 2nd most common cause of irreversible vision loss in the United States. Nearly 67 million people have the disease worldwide including >3 million in the United States. A major risk factor for POAG is an elevation in intraocular pressure (IOP). The increase in IOP is believed to be caused by an increase in the deposition of extracellular matrix proteins, in particular fibronectin, in a region of the eye known as the trabecular meshwork (TM). How fibronectin contributes to the increase in IOP is not well understood. The increased density of fibronectin fibrils is thought to increase IOP by altering the compliance of the trabecular meshwork. Recent studies, however, also suggest that the composition and organization of fibronectin fibrils would affect IOP by changing the cell-matrix signaling events that control the functional properties of the cells in the trabecular meshwork. In this article, we will discuss how changes in the properties of fibronectin and fibronectin fibrils could contribute to the regulation of IOP.

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Glaucomatous cell derived matrices differentially modulate non-glaucomatous trabecular meshwork cellular behavior

Cited by 64 publications

References 91 publications

The Molecular Processes in the Trabecular Meshwork After Exposure to Corticosteroids and in Corticosteroid-Induced Ocular Hypertension

The Molecular Processes in the Trabecular Meshwork After Exposure to Corticosteroids and in Corticosteroid-Induced Ocular Hypertension

From Shape to Function: The Next Step in Bioprinting

Role of Fibronectin in Primary Open Angle Glaucoma

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