Glaucocalyxin A inhibits the growth of liver cancer Focus and SMMC-7721 cells

Tang, Long; Jin, Xiaofeng; Hu, Xiaohui; Hu, Xiaoding; Liu, Zulong; Liu, Yu

doi:10.3892/ol.2015.4002

Cited by 13 publications

(5 citation statements)

References 26 publications

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“…Glaucocalyxin A (C 20 H 28 O 4 , MW: 332.44, Figure 2) Glaucocalyxin A dose-and concentration-dependently inhibited the growth of the liver cancer Focus and SMMC7721 cells. Mechanistic studies found induction of G2/M phase cell cycle arrest as well as increased expression of cleaved caspase-3 and PARP [55]. In MCF-7 and Hs578T breast cancer cells, glaucocalyxin A induced apoptosis, and G2/M phase arrest by increasing the expression of intrinsic apoptotic markers cleaved caspase-3, BAX, and p53, while decreasing BCL-2 expression.…”

Section: Glaucocalyxins a And Bmentioning

confidence: 95%

Mechanistic Pathways and Molecular Targets of Plant-Derived Anticancer ent-Kaurane Diterpenes

et al. 2020

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Since the first discovery in 1961, more than 1300 ent-kaurane diterpenoids have been isolated and identified from different plant sources, mainly the genus Isodon. Chemically, they consist of a perhydrophenanthrene subunit and a cyclopentane ring. A large number of reports describe the anticancer potential and mechanism of action of ent-kaurane compounds in a series of cancer cell lines. Oridonin is one of the prime anticancer ent-kaurane diterpenoids that is currently in a phase-I clinical trial in China. In this review, we have extensively summarized the anticancer activities of ent-kaurane diterpenoids according to their plant sources, mechanistic pathways, and biological targets. Literature analysis found that anticancer effect of ent-kauranes are mainly mediated through regulation of apoptosis, cell cycle arrest, autophagy, and metastasis. Induction of apoptosis is associated with modulation of BCL-2, BAX, PARP, cytochrome c, and cleaved caspase-3, -8, and -9, while cell cycle arrest is controlled by cyclin D1, c-Myc, p21, p53, and CDK-2 and -4. The most common metastatic target proteins of ent-kauranes are MMP-2, MMP-9, VEGF, and VEGFR whereas LC-II and mTOR are key regulators to induce autophagy.

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Section: Glaucocalyxins a And Bmentioning

confidence: 95%

Mechanistic Pathways and Molecular Targets of Plant-Derived Anticancer ent-Kaurane Diterpenes

et al. 2020

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“…Pharmacological studies have identified the bioactivities of Wangzaozins. For example, glaucocalyxin A has been widely studied for certain important biological activities, including antitumor [ 11 ], apoptosis induction [ 12 , 13 ], antibacterial, anti-oxidative, anticoagulative, antithrombotic, antifibrotic, immune, and antineuroinflammatory activities [ 5 , 14 , 15 ]. Wangzaozin A, specifically isolated from Wang Zao Zi and not from other Isodon species, has been proved to be effective in the treatment of pneumonia, lung abscess, and pulmonary tuberculosis when applied independently.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis

et al. 2018

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BackgroundIsodon amethystoides (Ben-th) Cy Wu et Hsuan is an important traditional medicinal plant endowed with pharmacological properties effective in the treatment of various diseases, including pulmonary tuberculosis. The tetracyclic diterpenoids, Wangzaozins (Wangzaozin A, glaucocalyxin A, glaucocalyxin B), are the major bioactive compounds of I. amethystoides. However, the molecular information about the biosynthesis of these compounds still remains unclear.ResultsAn examination of the accumulated levels of Wangzaozins in I. amethystoides revealed considerable variations in the root, stem, and leaf tissues of this plant, indicating possible differences in metabolite biosynthesis and accumulation among various tissues. To better elucidate the tetracyclic diterpenoid biosynthesis pathway, we generated transcriptome sequences from the root, stem, and leaf tissues, and performed de novo sequence assembly, yielding 230,974 transcripts and 114,488 unigenes, with average N50 lengths of 1914 and 1241 bp, respectively. Putative functions could be assigned to 73,693 transcripts (31.9%) based on BLAST searches against annotation databases, including GO, KEGG, Swiss-Prot, NR, and Pfam. Moreover, the candidate genes involving in the diterpenoid biosynthesis, such as CPS, KSL, were also analyzed. The expression profiles of eight transcripts, involving the tetracyclic diterpenoid biosynthesis, were validated in different I. amethystoides tissues by qRT-PCR, unraveling the gene expression profile of the pathway. The differential expressions of ISPD, ISPF and ISPH (MEP pathway), and IaCPS and IaKSL (diterpenoid pathway) candidate genes in leaves and roots, may contribute to the high accumulation of Wangzaozins in I. amethystoides leaves.ConclusionThe genomic dataset and analyses reported here lay the foundations for further research on this important medicinal plant.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1505-0) contains supplementary material, which is available to authorized users.

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“…20(S)‐ginsenoside Rh2 obtained from steroid glycosides like Ginseng dose‐ and time‐dependently induced apoptosis by caspase‐3, caspase‐8, and caspase‐9 activation in NB4 cells after a 12‐h treatment 57 . Glaucocalyxin A, when tested for its apoptotic ability in multiple cell lines, demonstrated strong anticancer effects in liver cancer Focus (IC 50 : 2.70 µM), SMMC‐7721 (IC 50 : 5.58 µM), HepG2 (IC 50 : 8.22 µM), SK‐HEP1 (IC 50 : 2.87 µM), 58 MCF‐7 (IC 50 : 1.00 µM), Hs578T (IC 50 : 4.00 µM), 59 and HL‐60. Several examples illustrated the effectiveness of terpenoids in inducing cytostatic effects, apoptosis, and activating caspase signaling pathways in different cancer cell lines.…”

Section: Role Of Phytochemicals As Caspase Inducers: Natural Semisynt...mentioning

confidence: 99%

Caspase‐driven cancer therapies: Navigating the bridge between lab discoveries and clinical applications

Allani,

Akhilesh,

Tiwari

2024

Cell Biochemistry & Function

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Apoptosis is the cell's natural intrinsic regulatory mechanism of normal cells for programmed cell death, which plays an important role in cancer as a classical mechanism of tumor cell death causing minimal inflammation without causing damage to other cells in the vicinity. Induction of apoptosis by activation of caspases is one of the primary targets for cancer treatment. Over the years, a diverse range of natural, synthetic, and semisynthetic compounds and their derivatives have been investigated for their caspase‐mediated apoptosis‐induced anticancer activities. The review aims to compile the preclinical evidence and highlight the critical mechanistic pathways related to caspase‐induced cell apoptosis in cancer treatment. The focus is placed on the key components of the mechanisms, including their chemical nature, and specific attention is given to phytochemicals derived from natural sources and synthetic and semisynthetic compounds. 180+ compounds from the past two decades with potential as anticancer agents are discussed in this review article. By summarizing the current knowledge and advancements in this field, this review provides a comprehensive overview of potential therapeutic strategies targeting apoptosis in cancer cells. The findings presented herein contribute to the ongoing efforts to combat cancer and stimulate further research into the development of effective and targeted anticancer therapies.

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Glaucocalyxin A inhibits the growth of liver cancer Focus and SMMC-7721 cells

Cited by 13 publications

References 26 publications

Mechanistic Pathways and Molecular Targets of Plant-Derived Anticancer ent-Kaurane Diterpenes

Mechanistic Pathways and Molecular Targets of Plant-Derived Anticancer ent-Kaurane Diterpenes

Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis

Caspase‐driven cancer therapies: Navigating the bridge between lab discoveries and clinical applications

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