Glass-supported lipid/polydiacetylene films for colour sensing of membrane-active compounds

Volinsky, Roman; Kliger, Mark; Sheynis, Tania; Kolusheva, Sofiya; Jelinek, Raz

doi:10.1016/j.bios.2006.12.024

Cited by 33 publications

(42 citation statements)

References 30 publications

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“…Figure 2 depicts the DCA results obtained for several pharmaceutical compounds (Table I) incubated on the DMPC/PDA film, revealing significant differences among the molecules tested. Figure 2 depicts the relationship between compound concentrations and the percentage of the red chromaticity shift (%RCS), which is a quantitative parameter measuring the extent of the blue-red transformation in the film surface (17). Amitriptyline and promethazine induced pronounced blue-red transitions at very low concentrations (<10 −4 M) (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Figure 1B depicts a typical colorimetric image of a lipid/ PDA film incubated around 30 min with increasing concentrations of acebutolol, a β-adrenoceptor blocking agent which exhibits a wide range of physiological effects associated with cell membrane interactions (23,24). The scanned image in The blue-red transformations occurring upon the film surface can be easily quantified through DCA, a simple image analysis algorithm designed to measure the abundance and intensity of the red pixels in a desired area within the scanned image (17). Figure 2 depicts the DCA results obtained for several pharmaceutical compounds (Table I) incubated on the DMPC/PDA film, revealing significant differences among the molecules tested.…”

Section: Resultsmentioning

confidence: 99%

“…Quantification of the blue-red color transformations was carried out using digital colorimetric analysis (DCA) (18). DCA was carried out by cropping the spots within the film images which the sample volume covered, and application of MATLAB® mathematical software for calculating the total intensity and abundance of red pixels on the surface (17).…”

Section: Methodsmentioning

confidence: 99%

“…Here we demonstrate the utilization of glass-supported lipid/ PDA thin films (17) for rapid colorimetric screening of the interactions of pharmaceutical compounds and semi-solid (gel) formulations with hydrophobic barriers. We show that PDAbased film systems offer significant advantages for practical pharmaceutical screening applications.…”

Section: Introductionmentioning

confidence: 99%

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Colorimetric Polymer Films for Predicting Lipid Interactions and Percutaneous Adsorption of Pharmaceutical Formulations

et al. 2008

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The colorimetric data yield insight into the degree of lipid binding of the molecules tested. The film assay is particularly advantageous for analysis of semi-solid (gel or lotion) formulations, elucidating the lipid interaction characteristics of specific molecular components within the mixtures. The new colorimetric film assay constitutes a generic, rapid, and easily applicable platform for predicting and screening interactions of pharmaceutical compounds and complex formulations with lipid barriers.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Colorimetric Polymer Films for Predicting Lipid Interactions and Percutaneous Adsorption of Pharmaceutical Formulations

et al. 2008

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“…The films produced were horizontally transferred to a glass surface modified with octadecyltrichlorosilane, which makes it hydrophobic. Hydrophobic layers were used in other studies to modify surfaces (Gaboriaud et al 2001;Guo et al 2007;Ma et al 2003;Scindia et al 2007;Volinsky et al 2007). …”

Section: Characterization Of Pda Films and Vesiclesmentioning

confidence: 99%

Polydiacetylene as a Biosensor: Fundamentals and Applications in the Food Industry

Pires

Soares

Silva

et al. 2008

Food Bioprocess Technol

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Biosensors offer the potential for real-time pathogen detection. Polydiacetylene (PDA) is an ideal choice for use as a sensor due to its unique optical properties. PDA molecules can form thin films or vesicles that change color from deep blue to red in response to different stimuli, like temperature, pH, and the presence of biological molecules. PDA films and vesicles have been proven to be promising devices for the detection of bacteria and bacterial toxins. Langmuir troughs (for films) and microcalorimetry (for vesicles) are among the many techniques used to characterize PDA films and vesicles. In order to enable more applications of PDA films, it is imperative to elucidate and gain a deeper understanding of the molecular interactions associated with their color change. In addition, it is necessary to transfer the PDA film onto a solid support like plastic in order to incorporate PDA biosensors into an intelligent packaging system. Through the use of such a system, consumers can monitor food quality, thereby preventing foodborne outbreaks. In this review, we discuss the formation of PDA films and vesicles, their characteristics, and their potential applications as biosensors. The possibility to incorporate PDA biosensors into an intelligent packaging system to be used in the food industry is also explored.

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Micro‐structured Devices for Chemical Sensing of Flavor and Fragrance

Schimmelpfennig

Dornbusch

Bannert

et al. 2008

Engineering in Life Sciences

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The aim of this study was to design, develop and test an integrated micro‐analytical system. Of special interest are micro‐fluidic and micro‐sensor applications in the field of chemical analysis, such as the optical detection of parameter changes, optical recognition of component profiles and technological micro‐reaction applications. For this purpose, a modular system was developed, which enables the realization of various application cases in an uncomplicated manner, and to execute (via serial or parallel combination of components) usually not compatible tasks. Software components were developed to control the measuring procedure as well as to execute the data interpretation up to a chemometrical discriminant analysis. Application is directed to the production and product control in life sciences mainly for food, natural products, cosmetics and pharmaceutics.

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Glass-supported lipid/polydiacetylene films for colour sensing of membrane-active compounds

Cited by 33 publications

References 30 publications

Colorimetric Polymer Films for Predicting Lipid Interactions and Percutaneous Adsorption of Pharmaceutical Formulations

Colorimetric Polymer Films for Predicting Lipid Interactions and Percutaneous Adsorption of Pharmaceutical Formulations

Polydiacetylene as a Biosensor: Fundamentals and Applications in the Food Industry

Micro‐structured Devices for Chemical Sensing of Flavor and Fragrance

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