Glass capillary column gas chromatography of phenol-alkylamines after flas-heater derivatization using a double injection technique

GC–MS determination of amphetamines in serum using on-line trifluoroacetylation

“…On average, inter-assay precision was only slightly higher than the within assay values: they were between 3.3 and 5.7% at 15 ng/mL (n = 12) and between 1.4 and 2.6% at 100 ng/mL (n = 12). Accuracy values at the concentration level of 15 ng/mL (n = 5) were between 94 and 100% for the five target compounds.…”

“…MBTFA could also be applied as a flash derivatization reagent. This technique was first applied by Christophersen et al [12]. For the determination of amphetamine-type drugs-of-abuse in biological samples only one study used this technique [13].…”

Simplified method for the chemical diagnosis of organic aciduria using GC/MS

“…To simplify the TMS derivatization, the flash-heater derivatization was applied; this technique is widely used for methylation of drugs [9], TMS derivatization of morphine [10] and TMS derivatization of fatty acid [11,22,23]. In the new method, a 1 l aliquot of the extract (ethyl acetate) was sandwiched between two plugs of the BSTFA + 1%TMCS in the injection syringe and injected into the glass liner with glass wool at 280 • C as mentioned in Section 2.2.2 (Flash-heater derivatization).…”

Derivatization in Mass Spectrometry—7. On-Line Derivatization/Degradation

“…49 A double injection derivatization technique has been applied to the GC/MS analysis of phenolalkylamines. 50 They were firstly O-trimethylsilylated by coinjection of the sample with MSTFA, then on-column acylated with N-methyl(triflu oroacetamide) introduced in a second injection.…”