Glandular trichomes on aerial and underground organs in Chrysolaena species (Vernonieae – Asteraceae): Structure, ultrastructure and chemical composition

Appezzato‐da‐Glória, Beatriz; Costa, Fernando B. Da; Silva, Vanessa Cristina da; Gobbo‐Neto, Leonardo; Rehder, Vera Lúcia Garcia; Hayashi, Adriana Hissae

doi:10.1016/j.flora.2012.10.003

Cited by 48 publications

(43 citation statements)

References 43 publications

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“…Biseriate glandular trichomes are typically found in Asteraceae, in different tribes and genera, such as Ambrosia trifida [44], Helianthus annuus [45] [46], Sigesbeckia jorullensis [41], Grindelia pulchella/Heliantheae [29], Centrapalus pauciflorus and Chrysolaena spp./Vernonieae [28] [47], respectively, Helichrysum [48] and Inula/Inuleae [49], Artemisia annua [50] and Tanacetum cinerariifolium/Anthemideae [51].…”

Section: Discussionmentioning

confidence: 99%

“…The observed striking difference, in addition to their differences in gland secretion compositions as observed in the present, may be involved in These unexpected results between two species that do not share populations [4], pose the question of whether the increased number of trichomes may be involved in organ-specific functions that may be relevant for their corresponding adaptations through habitat specialization [6]. The ultrastructure of the head cells of glandular trichomes revealed components typically found in cellular structures that produce and secrete metabolites [29] [55], although some glandular trichomes in Asteraceae only present smooth endoplasmic reticulum [28]. More specifically, the presence of abundant chloroplasts with visible starch grains in FC and FO is indicative of their functionality.…”

Section: Discussionmentioning

confidence: 99%

“…In Asteraceae, two secreting systems occur: glandular trichomes at the surface and secretory ducts inside the organs [28]- [30]. Glandular trichome characteristics have been used to classify species within the Asteraceae [31].…”

Section: Introductionmentioning

confidence: 99%

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Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Silva¹,

Tourn²,

López³

et al. 2015

AJPS

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In this work, the localization, density, morphology and ultrastructure of secretory structures in aerial organs of Flourensia campestris (FC) and F. oolepis (FO) (Asteraceae) by means of a combination of light, fluorescence, transmission (TEM) and scanning electron microscopy (SEM) were examined. The possible role of secretory structures in the production and secretion of the phytotoxic sesquiterpene (-)-hamanasic acid A ((-)HAA) in both species was also assessed. Capitate glandular trichomes were found in all reproductive organs of FC and FO, and were being reported for the first time. These glandular trichomes, typically associated to edges and veins, were of the same type as those already described for vegetative organs, and were abundant in involucral bracts and corolla of tubulose and ligulate flowers. Their density in reproductive organs of both species was similar (ca. 30/mm 2 ) and lower than that found in leaves (ca. 100/mm 2 ) and stems (ca. 160/mm 2 in FC, and up to 650/mm 2 in FO). Glandular trichomes in vegetative organs followed a species-* Corresponding authors. M. P. Silva et al. 926specific pattern of distribution. TEM and SEM observations suggest that each species differs in the way in which secretory materials are released to the outside: through cracks or pores in FC, or through a loose cuticle in FO. Similar inspections of the secretory ducts revealed lipophilic vacuoles localized in subepithelial and epithelial cells, in which secretions accumulated before being transferred to the duct. The presence of wall ingrowths in subepithelial cells suggests that granulocrine secretion operates in these species. Secretory ducts varied in density and diameter among the organs in both species, with the combination being maximal in woody stems. (-)HAA was only detected in surface secreted resins of both species, and its concentration (2D-TLC, GC-FID) was intimately associated with the distribution and density of glandular trichomes in each organ (capitula, leaves, and stems with primary or secondary growth). In addition, no (-)HAA was detected internally in the resins collected from secretory ducts. The composition of these resins showed distinctive profiles for FC and FO, and only four from ca. 30 compounds detected (GC/MS) were shared by both species. In addition to the elucidation of ultrastructural traits, distribution and density of secretory structures in aerial organs of FC and FO, present findings suggest a functional role for glandular trichomes in the secretion of the putative phytotoxic allelochemical (-)HAA.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Secretory Structures in Flourensia campestris and F. oolepis: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Silva¹,

Tourn²,

López³

et al. 2015

AJPS

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“…Substances of diff erent chemical categories can be produced by glandular trichomes, and their subcellular features vary according to the substances produced and the form of release of the secretion (Ascensão et al 1995;Ascensão & Pais 1998;Appezzato-da-Glória et al 2012;Naidoo et al 2012;Tozin et al 2015a;Silva et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Th e subcellular features of glandular trichomes have been exhaustively described to several species from diff erent families (Ascensão & Pais 1998;Sacchetti et al 1999;Argyropoulou et al 2010;Papini et al 2010;Paiva & Martins 2011;Appezzato-da-Glória et al 2012;Naidoo et al 2012;Amrehn et al 2014;Tozin et al 2015a;Silva et al 2016 and references therein); however, some important aspects, such as the involvement of the cytoskeletal elements in such secretory process remains poorly studied. It has been demonstrated that movement of oil droplets in the Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth.…”

Section: Introductionmentioning

confidence: 99%

Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth. (Lamiaceae) and differential labeling of cytoskeletal elements

Tozin

Rodrigues

2016

Acta Bot. Bras.

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Lamiaceae contains many species known for their aromatic properties that are produced by the production of essential oils in glandular trichomes. Hyptis is one of the most common genera of Lamiaceae in the Brazilian fl ora, and includes several species with medicinal value. However, studies on the morphology and functioning of their glandular trichomes are lacking. We analyzed the morphology, histochemistry and ultrastructure of the glandular trichomes in leaves of H. villosa, emphasizing the diff erential distribution of actin fi laments and microtubules in cells secreting hydrophilic and lipophilic compounds. Four morphotypes of glandular trichomes were identifi ed. Total lipid, terpenes, alkaloids, phenolic compounds, proteins and polysaccharides were histochemically detected in all morphotyes. Th is evidences the mixed nature of the secretions of this species, although there are diff erences in the prevalence of lipophilic and hydrophilic components among the glandular morphotypes and among the cells of the same trichome. Th e actin microfi laments are more abundant in cells that secrete mainly hydrophilic compounds, and microtubules predominate in cells that secrete lipophilic compounds. Our results corroborate the correlation between the glandular morphotype and the composition of the secretion produced, with a diff erential distribution of the cytoskeletal elements according to the prevalence of either hydrophilic or lipophilic substances.

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Comparative ultrastructure of trichomes on various organs of Rosa roxburghii

Wang

Lü

Ludlow

et al. 2021

Microscopy Res & Technique

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Chestnut rose, R. roxburghii Tratt. (Rosaceae) (RR) is an important crop in China due to its nutritional and medicinal values. RR frequently produces trichomes on the surfaces of a diverse range of organs, however a genetic component exists to the control of trichome development, with some cultivars having significantly fewer trichomes to others. Certain varieties have fruits that are thickly covered with macroscopic trichomes, which is an undesirable trait for fruit processing and consumption. However, smooth‐fruit cultivars exist, such as R. roxburghii Tratt. f. esetosa Ku (RRE). Despite their economic importance, the anatomical features of trichomes have not been explored in detail for these two chestnut rose germplasms. Here, we investigate the ultrastructure of trichomes distributed on the stem, sepal, and fruit of RR and RRE using transmission electron microscopy (TEM). The internal structure of stem prickle trichomes in RR and RRE was oval in shape and did not contain nucleoli or other organelles. The cell walls of stem prickles in RR are thick and the intercellular spaces occupied with liquid, whereas the cells wall of stem prickles in RRE are thin and have air‐filled intercellular spaces. The cells of sepal acicular trichomes in RR and glandular trichomes (GTs) of sepals in RRE had similar vacuole sizes, cytoplasm content, intercellular spaces, and arrangement of plastids within cells. However, there were osmiophilic granules present in the GTs of RRE. The flagelliform trichomes in the sepals of the two germplasms are composed of oval or rod‐shaped cells. Although the flagelliform trichomes in the sepals of the two germplasms had a similar internal structure, and both contained starch grains and plastids with visible thylakoid membranes, the flagelliform trichomes in the sepals of RR had a thinner cell wall and a higher proportion of cytoplasm which was more evenly distributed across the cell. There were granules that stained heavily with osmium tetroxide which occurred infrequently in the flagelliform trichomes of sepals in RRE but were not observed in RR. On the acicular trichomes of fruit in RR, the flagelliform trichomes and the GTs of fruit in RRE shared similar cell morphology, arrangement and vacuole size as well as intercellular space. Both the fruit flagelliform trichomes and GTs in RRE contain granules which stain heavily with osmium tetroxide, and the GTs contain plastids and starch grains. These differences in trichome cell ultrastructure may be related to developmental processes or biological functions of the trichomes. These results also suggest that the two chestnut rose germplasms are good candidates for further study of trichome ontogeny in the genus and subsequent breeding of the smooth organ trait in this species.

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Glandular trichomes on aerial and underground organs in Chrysolaena species (Vernonieae – Asteraceae): Structure, ultrastructure and chemical composition

Cited by 48 publications

References 43 publications

Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth. (Lamiaceae) and differential labeling of cytoskeletal elements

Comparative ultrastructure of trichomes on various organs of Rosa roxburghii

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Glandular trichomes on aerial and underground organs in Chrysolaena species (Vernonieae – Asteraceae): Structure, ultrastructure and chemical composition

Cited by 48 publications

References 43 publications

Secretory Structures in &lt;i&gt;Flourensia campestris&lt;/i&gt; and &lt;i&gt;F. oolepis&lt;/i&gt;: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Secretory Structures in &lt;i&gt;Flourensia campestris&lt;/i&gt; and &lt;i&gt;F. oolepis&lt;/i&gt;: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth. (Lamiaceae) and differential labeling of cytoskeletal elements

Comparative ultrastructure of trichomes on various organs of Rosa roxburghii

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Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion

Secretory Structures in <i>Flourensia campestris</i> and <i>F. oolepis</i>: Ultrastructure, Distribution, and (-)-Hamanasic Acid A Secretion