GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum

Kosmala, Arkadiusz; Zwierzykowski, Z.; Gasior, Dagmara; Rapacz, Marcin; Zwierzykowska, E.; Humphreys, Martin

doi:10.1038/sj.hdy.6800787

Cited by 60 publications

(60 citation statements)

References 38 publications

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“…This implies that introgression of some desirable LongiXorum traits into an Asiatic cultivar could be realized at BC2 level. Similar results have been found in Festuca-Lolium intergeneric hybrids where freezing tolerance genes were successfully introgressed into Lolium multiXorum from Festuca pratensis in a back cross breeding programme (Kosmala et al 2006).…”

Section: Discussionsupporting

confidence: 72%

Potential for analytic breeding in allopolyploids: an illustration from Longiflorum × Asiatic hybrid lilies (Lilium)

et al. 2008

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Ploidy level and intergenomic recombination was studied in interspeciWc hybrids between LongiXorum £ Asiatic lilies (LA hybrid) backcross to Asiatic parents in order to assess the possibility for analytic breeding in lily. By backcrossing the diploid (2n = 2x = 24) F1 interspeciWc hybrid between LongiXorum £ Asiatic lilies to Asiatic parents, 104 BC1 progeny plants were produced. Among these, there were 27 diploids, 73 triploids (2n = 2x = 36) and 4 aneuploids (2x ¡ 1, 2x + 2 or 2x + 3). In addition, by backcrossing triploid BC1 (LAA) plants to diploid Asiatic parents in 2x ¡ 3x and reciprocal combinations, 14 diploid BC2 progenies were produced. Genomic in situ hybridization (GISH) was performed to study the intergenomic recombination and karyotype composition. GISH indicated extensive intergenomic recombination among the chromosomes in LA hybrids. A large number of LongiXorum chromosomes were transmitted to the BC1 progenies from LA hybrids. However, very few LongiXorum chromosomes were transmitted from the BC1 triploid (LAA) plants to the BC2 progenies. The occurrence of diploid plants in the BC progenies of LA hybrids has opened the prospects of analytic breeding in lilies. In this approach, the selection of superior genotypes can be carried out at the diploid level and polyploid forms are synthesized from superior diploid parents. The advantages of analytic breeding are evident: (a) a maximum level of heterozygosity can be attained in the synthetic polyploids and (b) introgression can be achieved with a minimum of linkage drag. Based on GISH results the potential application of analytic breeding in lily allopolyploids has been discussed.

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Section: Discussionsupporting

confidence: 72%

Potential for analytic breeding in allopolyploids: an illustration from Longiflorum × Asiatic hybrid lilies (Lilium)

et al. 2008

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“…Recently, analysis of genomic introgression between winter susceptible Lolium multiflorum and the winter tolerant species Festuca pratensis revealed that a derived freezing-tolerant genotype contained a Festuca introgression segment in a terminal region of Lolium chromosome 2 (Kosmala et al 2005). As LG2 of perennial ryegrass is the syntentic counterpart of the Triticeae group 2 chromosomes, the observation of QTL-candidate gene co-location on LG2, as well as associated functional data reported in this study, may indicate that some of the genes commonly showing allelic variation for winter hardiness differ between the pasture grasses and the cereals.…”

Section: Discussionmentioning

confidence: 99%

Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation

et al. 2006

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A perennial ryegrass cDNA clone encoding a putative glycine-rich RNA binding protein (LpGRP1) was isolated from a cDNA library constructed from crown tissues of cold-treated plants. The deduced polypeptide sequence consists of 107 amino acids with a single N-terminal RNA recognition motif (RRM) and a single C-terminal glycine-rich domain. The sequence showed extensive homology to glycine-rich RNA binding proteins previously identified in other plant species. LpGRP1-specific genomic DNA sequence was isolated by inverse PCR amplification. A single intron which shows conserved locations in plant genes was detected between the sequence motifs encoding RNP-1 and RNP-2 consensus protein domains. Significant increase in the mRNA level of LpGRP1 was detected in root, crown and leaf tissues during the treatment of plants at 4°C, through which freezing tolerance is attained. The increase in the mRNA level was prominent at least 2 hr after commencement of the cold treatment, and persisted for at least one week. Changes in mRNA level induced by cold treatment were more obvious than those due to treatments with abscisic acid (ABA) and drought. The LpGRP1 protein was found to localize in the nucleus in onion epidermal cells, suggesting that it may be involved in pre-mRNA processing. The LpGRP1 gene locus was mapped to linkage group 2. Possible roles for the LpGRP1 protein in adaptation to cold environments are discussed.

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“…Although genetic exchange occurs freely between Festuca and Lolium chromosomes, there is a tendency for Lolium chromatin to progressively dominate over successive generations. Nevertheless, mechanisms exist for the potentially stable introgression of genes from one genus to the other (in either direction) to create cultivars with the genetic background of one species but carrying important traits from the other (e.g., Kosmala et al 2006). similar scope exists for hybrids involving Lolium withhexaploidF.…”

Section: Awareness and Use Of Secondary Gene-poolsmentioning

confidence: 99%

Future options and targets for pasture plant breeding in New Zealand

Williams

Easton

Jones

2007

New Zealand Journal of Agricultural Research

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Industries based on pastoral farming have increased their contribution to GDP from 13.5 to 17% since 1990 as the result of markedly intensified farming practices. In the future, we predict that this intensification will continue but, at the same time, there will be an emergence of an efficient, lower-input farming sector with almost no environmental footprint. Both sectors will require continuing input by pasture plant breeders. Over the past 20 years, development of pasture cultivars has become totally industry funded, with support from Crown funding for basic research. There have been several key advances in pasture plant breeding including new methods for using exotic germplasm and secondary gene-pools, modification of grass-endophyte associations, breeding for specific environments and the successful adoption of international breeding programmes. The emergence of genomics, marker-assisted selection (MAS) and genetic modification (GM) offer considerable promise for future development of pasture cultivars. Future grass breeding, aided by MAS and GM of both plants and endophytes, will place strong emphasis on feeding value for optimal animal performances, especially in intensive systems. There will also be development of grass types adapted to efficient, lower-input farming systems that will have minimal environmental A07026;

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GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum

Cited by 60 publications

References 38 publications

Potential for analytic breeding in allopolyploids: an illustration from Longiflorum × Asiatic hybrid lilies (Lilium)

Potential for analytic breeding in allopolyploids: an illustration from Longiflorum × Asiatic hybrid lilies (Lilium)

Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation

Future options and targets for pasture plant breeding in New Zealand

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