Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C

Zhang, Dalei; Wang, Kaiming; Zhang, Caiqiao

doi:10.1631/jzus.b0820133

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…Combined administration of PMA and FSH resulted in a visible increase in the germ cell number and LI. These conclusions were consistent with the viewpoints that the treatment of ovarian germ cells and mouse spermatogonia with PMA and ginsenosides promoted the cell proliferation involving the PKC-mediated system Zhang et al, 2009). Hence, we propose that PKCmediated system is involved in the FSH-stimulated cell proliferation in cultured chicken ovarian germ cells.…”

Section: Discussionsupporting

confidence: 92%

Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

Liu

Zeng

Cao

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways. Ovarian cells were treated with FSH alone or in the presence of forskolin (FRSK), PKA inhibitor (H 89 ), PKC activator (PMA) or inhibitor (H 7 ). The germ cell number was counted from micropictures. The immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating cells. The germ cell labeling index (LI) was determined for cell proliferation. The FSH treatment increased the germ cell number, and this stimulating effect was enhanced by FRSK or PMA, but inhibited by H 89 or H 7 in a dose-dependent manner. Moreover, the PCNA-LI showed parallel changes with germ cell numbers. This study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.

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Section: Discussionsupporting

confidence: 92%

Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

Liu

Zeng

Cao

et al. 2010

J. Zhejiang Univ. Sci. B

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“…Ginsenosides are the primary components of ginseng and positively affect reproductive organs and other tissues [20,22]. The supplementation of WG at WG2 in the current study improved FCR, HDEP, AEW, EM, TNF-, yolk ginsenoside content, and microflora DNA in the last phase of our experiment but had no positive effect on ADFI, BW, and egg quality.…”

Section: Discussionmentioning

confidence: 44%

Effect of wild ginseng on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of laying hens

Tajudeen¹,

Mun²,

Ha³

et al. 2022

J Anim Sci Technol

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The experiment was carried out to study the effect of Korean wild ginseng adventitious root supplementation on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of Institut de selection Animale (ISA) brown laying hens at 24 weeks old. A total of 90 laying hens were subjected to a completely randomized design at three treatments, five repetitions and six laying hens per replicate. The experiments were divided by diets into the basic feed (CON), basic feed + 0.1% wild ginseng (WG1), and basic feed + 0.5% wild ginseng (WG2). The feeding trial was carried out over a duration of 12 weeks after an initial acclimation period of 2 weeks. Feeds and water were administered ad libitum in mash form, and light was available for 16 hours per day. At the end of study, hen-day egg production (HDEP), average egg weight (AEW), and egg mass (EM) were increased (p <0.05) in WG2 at week 12. Feed conversion ratio (FCR) was decreased (p <0.05) in WG2 at week 12. The ginsenoside content in egg yolk was increased (p <0.05) in laying hens in the WG2 treatment at week 12. Relative expression of tumor necrosis factor alpha (TNF-α) was reduced (p <0.05) in the WG supplemented diets at week 12.The fecal microflora quantity of Lactobacillus was increased (p <0.05) in WG2 at week 8 to week 12, and Escherichia coli (E. coli) was significantly decreased (p <0.05) in the WG2 at week 12. We concluded that the result observed in the HDEP, AEW, EM and FCR was due to an increase in ginsenoside content, leading to an improvement in the TNF-α, and fecal microflora quantity such as Lactobacillus and E. coli in the WG2 supplemented diets. We therefore recommend the use of WG at application level 0.5% per basal diet for optimum laying performance in layer hens.

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“…GS are the main molecular components responsible for the actions of ginseng and exert varying effects on a myriad of cells and tissues. Literature reports suggest that GS could play a positive role in reproduction (Salvati et al, 1996; Kang et al, 2002; Yu et al, 2003; Zhang et al, 2009). Our laboratory reported that GS stimulate murine oocyte meiotic maturation indirectly via the cumulus cells.…”

Section: Discussionmentioning

confidence: 99%

“…Kang et al (2002) reported that ginseng intestinal metabolite‐I reduced doxorubicin toxicity in the mouse testis. Furthermore, a previous study had demonstrated the direct proproliferating effect of GS on mouse spermatogonia and chicken primordial germ cells through PKC (protein kinase C) activation (Ge et al, 2007; Zhang et al, 2009). In females, GS could ameliorate ovarian dysfunction that was caused by excessive stimulation by equine chorionic gonadotropin in immature rats (Yu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Ginsenosides promote proliferation of granulosa cells from chicken prehierarchical follicles through PKC activation and up‐regulated cyclin gene expression

Tan

et al. 2010

Cell Biology International

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The effect of GS (ginsenosides) on proliferation of chicken GCs (granulosa cells) from prehierarchical SYF (small yellow follicles) was evaluated, and involvement of the PKC (protein kinase C) signalling pathway as well as mRNA expression of cyclins and CDK (cyclin-dependent kinase) were investigated. Whole SYF or GCs isolated from SYF were cultured in Medium 199 supplemented with 0.5% FCS (fetal calf serum). After 16 h, the cells were challenged with GS alone or in combination with PKC inhibitor H7 or activator PMA (phorbol 12-myristate 13-acetate) for 24 h in serum-free medium. Results showed that in both whole follicles and pure GCs monolayer culture system, GS (0.1-10 microg/ml) significantly increased the number of GCs in SYF in a dose-dependent manner, and this stimulatory effect was inhibited by H7, but enhanced by PMA. Meanwhile, the PCNA-LI (proliferating cell nuclear antigen labelling index) of GCs displayed similar changes with the cell number. Mechanism of GS action was further evaluated in cultured GCs separated from SYF. Western blot analysis showed that 10 microg/ml GS increased PKC translocation from cytoplasm to the plasma membrane of the GCs to become the active state. This effect was blocked by H7. Furthermore, GS up-regulated the expression of cyclin D1/CDK6 and cyclin E/CDK2 mRNAs in GCs; however, inhibition of PKC with H7 attenuated this stimulatory effect. These results indicated that GS could stimulate proliferation of chicken GCs through activated PKC-involved up-regulation of cyclin D1/CDK6 and cyclin E/CDK2 genes, subsequently promoting development of the chicken prehierarchical follicles.

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Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C

Cited by 7 publications

References 18 publications

Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

Effect of wild ginseng on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of laying hens

Ginsenosides promote proliferation of granulosa cells from chicken prehierarchical follicles through PKC activation and up‐regulated cyclin gene expression

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