Ginsenoside Rh2 induces Bcl‐2 family proteins‐mediated apoptosis <i>in vitro</i> and in xenografts <i>in vivo</i> models

Choi, Sunga; Oh, Jun-Young; Kim, Soojin

doi:10.1002/jcb.22932

Cited by 52 publications

(30 citation statements)

References 42 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The research proved that the mainly active ingredients of anti-tumor in ginseng is ginsenosides (Choi S et al, 2011;Tang XP et al, 2013) , through the comparison of various kinds of ginsenoside found that GS-Rh2 has the strongest ability to inhibited the proliferation of cancer cells (Li B et al, 2011).…”

Section: Introductionmentioning

confidence: 96%

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Qian¹,

Li²,

Jia³

et al. 2016

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 96%

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Qian¹,

Li²,

Jia³

et al. 2016

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

“…The tumor suppressive ability of G-Rh2 is related to the inhibition of cell proliferation and metastasis, and induction of apoptosis . However, studies using different cell line models have reported conflicting data (Choi et al, 2011;Dong et al, 2011;Chung et al, 2013;Tang et al, 2013;Fatmawati et al, 2014;Guo et al, 2014;You et al, 2014a). We previously reported that G-Rh2 can inhibit the proliferation of human lung adenocarcinoma A549 cells as well as cause cell cycle arrest in the G1 phase in a dose-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

JNK pathway and relative transcriptional factor were involved in ginsenoside Rh2-mediated G1 growth arrest and apoptosis in human lung adenocarcinoma A549 cells

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Ginsenoside Rh2 has been shown to have an antitumor effect on a wide range of cancers. A previous study has shown that ginsenoside Rh2 can inhibit the proliferation of the human lung adenocarcinoma A549 cell line in a dose-dependent manner by activating caspase-8/3 activity to promote apoptosis. However, the association of the JNK signaling pathways and transcription factors with ginsenoside Rh2 in the suppression of non-small cell lung cancer has not yet been reported. In this study, we found that ginsenoside Rh2 can activate the JNK/MAPKs signaling pathway and increase the phosphorylation and transcriptional activity of the transcription factors AP-1 and ATF2. Ginsenoside Rh2 also reduced the expression of transcription factors E2F1 and c-Myc. Furthermore, ginsenoside Rh2 affected the expression levels of cyclin D1 and the CDK4 protein, which are key regulatory factors of the G1/S cyclin-dependent kinase. The anti-proliferative and induced apoptotic effects of ginsenoside Rh2 on A549 cell provide evidence to support the application of traditional Chinese medicine to lung cancer treatment.

show abstract

“…27) In the mitochondrial apoptosis pathway, G-Rh2 administration accelerated the translocation of Bax and Bak from cytosol to mitochondria which consequently stimulated the release of cytochrome c and initiated apoptotic process. 28) In this study, we found that ER stress induced apoptosis also was an alternative pathway that G-Rh2 could induce cell death in lung cancer.…”

Section: Discussionmentioning

confidence: 70%

Ginsenoside Rh2 Inhibited Proliferation by Inducing ROS Mediated ER Stress Dependent Apoptosis in Lung Cancer Cells

Gao

Yan

Cai

2017

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Key words ginsenoside Rh2; lung cancer; apoptosis; reactive oxygen species; endoplasmic reticulum stress Followed by serial biological steps including hyperplasia, metaplasia, dysplasia and in situ carcinoma, similar to other cancers, lung cancer eventually develops its malignancy marked by strong proliferative capacity which is characterized by resistance to apoptosis. 1) Nowadays, lung cancer is still among the top common cancers worldwide. According to the records, lung cancer composes 12.7% of cancer and 18.2% of cancer related death.2) Though the multiple therapies including surgery, radiotherapy and chemotherapy are more and more widely applied to patients, the prognosis of lung cancer is still poor due to its malignant nature. Non-small cell lung cancer (NSCLC) takes approximately 85% of lung cancer cases with less than 20% 5-year survival rate.3) Seeking and identifying new drugs inducing apoptosis may provide us new insight into prevention and treatment of lung cancer.As one of the vital organelles in eukaryotic cells, endoplasmic reticulum (ER) is responsible for directing correct protein folding, calcium homeostasis and lipid metabolism. 4) However, under certain physiological and pathological conditions, the normal functions of ER were impaired by multiple extra-or intra-cellular stimuli, which is referred as ER stress.5) Reactive oxygen species (ROS) is considered as one of the stimuli inducing ER stress. This phenotype is called ROS-mediated ER stress. 6) Many anticancer agents such as chemotherapeutic drugs are believed to induce apoptosis by generating excessive intracellular ROS which is related with unfolded protein response (UPR) in ER to induce ER stress. 7) Numerous investigations supposed that ROS-mediated ER stress could further promote cell apoptosis by activating CCAAT/enhancer binding protein (C/EBP) homologous protein CHOP signaling pathway.8) As a pre-apoptotic factor, CHOP is suggested to activate caspase cascade by inducing cleavage of caspase-12 (rodents) 9) or caspase-4 (human). 10)In recent decades, natural products gradually become new alternatives besides regular therapies. With a long history in traditional medicine in East Asia, ginseng (Panax quinquefolius) is now a very popular herb as medicine or food supplement in the world. 11) Extracted from roots of ginseng, ginsenosides are the main effective components.12) It was suggested in the previous study that among the ginsenosides, ginsenoside Rh2 (G-Rh2, first isolated by Shizuo Odashima 13) ) exhibited various biological and pharmacological activities including anti-inflammation, anti-diabetes and anti-cancer. 14-17)A recent report supposed that G-Rh2 induced colorectal cancer cell apoptosis by inducing excessive intracellular ROS generation.18) Thus, ROS-mediated ER-stress induced apoptosis attracted our attention in investigating gensenoside Rh2's mechanism of anti-proliferation.In this present study, we showed G-Rh2 inhibited proliferation of lung cancer cells by inducing apoptosis. Furthermore, whether ROS-mediated ER-s...

show abstract

Ginsenoside Rh2 induces Bcl‐2 family proteins‐mediated apoptosis in vitro and in xenografts in vivo models

Cited by 52 publications

References 42 publications

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

JNK pathway and relative transcriptional factor were involved in ginsenoside Rh2-mediated G1 growth arrest and apoptosis in human lung adenocarcinoma A549 cells

Ginsenoside Rh2 Inhibited Proliferation by Inducing ROS Mediated ER Stress Dependent Apoptosis in Lung Cancer Cells

Contact Info

Product

Resources

About