Ginsenoside Rg5 alleviates Ang II–induced cardiac inflammation and remodeling by inhibiting the JNK/AP-1 pathway

Yu, Tianxiang; Xu, Xiaoli; Wei, Jiajia; Xu, Jingcheng; Luo, Wei; Li, Ankang; Liang, Guang; Wang, Mengyang

doi:10.1016/j.intimp.2023.110408

Cited by 9 publications

(6 citation statements)

References 22 publications

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“…Cells were cultured in Dulbecco's modified Eagle medium, 10% fetal bovine serum (Gibco, USA) was mixed with 100 U/mL penicillin/streptomycin (Corning, USA) in 5% CO 2 at 37°C. Increased level of angiotensin II (Ang II) contributes to hypertensive HF via haemodynamic and non‐haemodynamic actions 18 . In the current experiments, we incubated HL‐1 cells with medium containing 100 nmol/L Ang II (Sigma‐Aldrich, Saint Louis, MO, USA) for 48 h to induce an endocrine milieu in vitro to mimic HF and constructed HL‐1 apoptosis model 19 .…”

Section: Methodsmentioning

confidence: 99%

“…Increased level of angiotensin II (Ang II) contributes to hypertensive HF via haemodynamic and non‐haemodynamic actions. 18 In the current experiments, we incubated HL‐1 cells with medium containing 100 nmol/L Ang II (Sigma‐Aldrich, Saint Louis, MO, USA) for 48 h to induce an endocrine milieu in vitro to mimic HF and constructed HL‐1 apoptosis model. 19 The serum‐free medium containing Ang II was raplaced every 24 h.…”

Section: Methodsmentioning

confidence: 99%

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miRNA‐103‐3p‐Hlf regulates apoptosis and autophagy by targeting hepatic leukaemia factor in heart failure

et al. 2023

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AimsCardiomyocyte apoptosis is an important factor leading to the occurrence and development of heart failure (HF), which is associated with high mortality of patients with cardiovascular diseases. This study aims to investigate the underlying mechanisms of HF in terms of expression and regulation patterns using bioinformatics and experimental validation.Methods and resultsTwo HF datasets were collected: a dataset GSE112056 downloaded from the GEO database (including mRNA and miRNA sequencing data) and another is the laboratory‐owned mRNA dataset. Differential mRNAs and miRNAs in the two datasets were screened using the raw Bayesian approach method. Gene Ontology was used to perform functional enrichment analysis of the differential mRNAs and co‐expression network analysis of the differential mRNAs, combined with nuclear transcription factors in the differential miRNAs and mRNAs for target gene prediction. A HF cell model was constructed using mouse cardiomyocytes (HL‐1), and the role and mechanism of miRNA‐103‐3p‐Hlf (hepatic leukaemia factor) in the process of HF was verified by cell transfection, luciferase reporter gene, WB, and qPCR. We found that Hlf gene expression was decreased in the HF model group and strongly correlated with FYCO1 (FYVE and coiled‐coil domain‐containing protein 1) gene, a phenomenon enriched in apoptotic autophagy‐related pathways. MiR‐103‐3p expression was up‐regulated in the HF model group, and its targeting correlation with Hlf was confirmed by luciferase activity assay. In the HL‐1 cell model, miR‐103‐3p significantly promoted apoptosis and inhibited autophagy in HL‐1 cells (all P < 0.05), and overexpression of the Hlf gene reversed this phenomenon, inhibiting apoptosis and promoting autophagy in HL‐1 cells (all P < 0.05).ConclusionsMiR‐103‐3p affects myocardial cells apoptosis and autophagy by targeting Hlf, playing as a potential therapeutic biomarker for HF progression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

miRNA‐103‐3p‐Hlf regulates apoptosis and autophagy by targeting hepatic leukaemia factor in heart failure

et al. 2023

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“…Prophylactic supplementation of ginseng protected from cardiac dysfunction in chemotherapy, similarly ginsenoside Rg1 ameliorated cardiac remodeling in mice suggesting the potential effect of ginsenosides against cardiac damage [ 12 ]. Also, ginsenoside Rg5 inhibited cardiac inflammation and protected the hearts of angiotensin II induced mice in hypertensive heart failure [ 13 ]. SARS-CoV-2 primarily affects the pulmonary system, but it could spread to heart and other organs.…”

Section: Introductionmentioning

confidence: 99%

Ginseng and ginsenosides on cardiovascular and pulmonary diseases; Pharmacological potentials for the coronavirus (COVID-19)

Vijayakumar,

Kim

2024

Journal of Ginseng Research

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“…The rare ginsenosides Rg3(S), Rg3(R), Rk1, and Rg5 have strong biological activities, such as anti-tumor [1][2][3][4], ameliorate myocardial glucose metabolism, and insulin resistance [5,6], suppress platelet mediated thrombus formation [7], alleviate inflammation [8][9][10], and ameliorate allergic airway inflammation and oxidative stress [11]. They are mainly obtained from Panax ginseng.…”

Section: Introductionmentioning

confidence: 99%

The Content and Principle of the Rare Ginsenosides Produced from Gynostemma pentaphyllum after Heat Treatment

Li,

Pei

et al. 2023

Molecules

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Ginsenoside Rg3, Rk1, and Rg5, rare ginsenosides from Panax ginseng, have many pharmacological effects, which have attracted extensive attention. They can be obtained through the heat treatment of Gynostemma pentaphyllum. In this study, scanning electron microscopy (SEM) and thermal gravity-differential thermal gravity (TG-DTG) were employed to investigate this process and the content change in ginsenosides was analyzed using liquid chromatography-mass spectrometry (LC-MS). SEM and TG-DTG were used to compare the changes in the ginsenosides before and after treatment. In SEM, the presence of hydrogen bond rearrangement was indicated by the observed deformation of vascular bundles and ducts. The before-and-after changes in the peak patterns and peaks values in TG-DTG indicated that the content of different kinds of compounds produced changes, which all revealed that the formation of new saponins before and after the heat treatment was due to the breakage or rearrangement of chemical bonds. Additionally, the deformation of vascular bundles and vessels indicated the presence of hydrogen bond rearrangement. The glycosidic bond at the 20 positions could be cleaved by ginsenoside Rb3 to form ginsenoside Rd, which, in turn, gave rise to ginsenoside Rg3(S) and Rg3(R). They were further dehydrated to form ginsenoside Rk1 and Rg5. This transformation process occurs in a weak acidic environment provided by G. pentaphyllum itself, without the involvement of endogenous enzymes. In addition, the LC-MS analysis results showed that the content of ginsenoside Rb3 decreased from 2.25 mg/g to 1.80 mg/g, while the contents of ginsenoside Rk1 and Rg5 increased from 0.08 and 0.01 mg/g to 3.36 and 3.35 mg/g, respectively. Ginsenoside Rg3(S) and Rg3(R) were almost not detected in G. pentaphyllum, and the contents of them increased to 0.035 and 0.23 mg/g after heat treatment. Therefore, the rare ginsenosides Rg3(S), Rg3(R), Rk1, and Rg5 can be obtained from G. pentaphyllum via heat treatment.

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Ginsenoside Rg5 alleviates Ang II–induced cardiac inflammation and remodeling by inhibiting the JNK/AP-1 pathway

Cited by 9 publications

References 22 publications

miRNA‐103‐3p‐Hlf regulates apoptosis and autophagy by targeting hepatic leukaemia factor in heart failure

miRNA‐103‐3p‐Hlf regulates apoptosis and autophagy by targeting hepatic leukaemia factor in heart failure

Ginseng and ginsenosides on cardiovascular and pulmonary diseases; Pharmacological potentials for the coronavirus (COVID-19)

The Content and Principle of the Rare Ginsenosides Produced from Gynostemma pentaphyllum after Heat Treatment

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