Ginsenoside Re Treatment Attenuates Myocardial Hypoxia/Reoxygenation Injury by Inhibiting HIF-1α Ubiquitination

Sun, Huiyuan; Ling, Shukuan; Zhao, Di; Li, Jianwei; Li, Yang; Qu, Hua; Du, Ronghui; Zhang, Ying; Xu, Feng; Liu, Caizhi; Zhong, Guangming; Liang, Shuai; Liu, Zizhong; Gao, Xingcheng; Jin, Xiaoyan; Li, Yingxian; Shi, Donglei

doi:10.3389/fphar.2020.532041

Cited by 15 publications

(5 citation statements)

References 28 publications

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“…In addition, in the hypoxia/reoxygenation injury model, Re (100 µM for 21 h) increased HL-1 cell viability and ATP levels. The possible mechanism was that Re acted on the binding interface between HIF-1α and von Hippel-Lindau protein to prevent the binding of these proteins, thereby suppressing HIF-1α ubiquitination ( Sun et al, 2020 ).…”

Section: Pharmacological Effects Of Re On Cardiovascular Diseases (Cvds)mentioning

confidence: 99%

Pharmacological Properties of Ginsenoside Re

et al. 2022

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Ginsenoside Re is a protopanaxatriol-type saponin extracted from the berry, leaf, stem, flower bud, and root of Panax ginseng. In recent years, ginsenoside Re (Re) has been attracting attention as a dietary phytochemical. In this review, studies on Re were compiled by searching a combination of keywords, namely “pharmacology,” “pharmacokinetics,” and “toxicology,” in the Google Scholar, NCBI, PubMed, and Web of Science databases. The aim of this review was to provide an exhaustive overview of the pharmacological activities, pharmacokinetics, and toxicity of Re, focusing on clinical evidence that has shown effectiveness in specific diseases, such as diabetes mellitus, nervous system diseases, inflammation, cardiovascular disease, and cancer. Re is also known to eliminate virus, enhance the immune response, improve osteoporosis, improve skin barrier function, enhance intracellular anti-oxidant actions, regulate cholesterol metabolism, alleviate allergic responses, increase sperm motility, reduce erectile dysfunction, promote cyclic growth of hair follicles, and reduce gastrointestinal motility dysfunction. Furthermore, this review provides data on pharmacokinetic parameters and toxicological factors to examine the safety profile of Re. Such data will provide a theoretical basis and reference for Re-related studies and future applications.

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Section: Pharmacological Effects Of Re On Cardiovascular Diseases (Cvds)mentioning

confidence: 99%

Pharmacological Properties of Ginsenoside Re

et al. 2022

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“…GRe is critical for the pharmacological activities of ginseng, and similar to other ginsenosides, it has attracted increasing attention for possible use in the treatment of metabolic diseases (Huang et al, 2019). Previous studies have shown that GRe exerts multiple beneficial effects to attenuate cell damage (Sun et al, 2020), reduce the apoptosis rate (Liu et al, 2002) and increase antioxidant activity (Liu et al, 2019). Our study indicates that GRe enhances the quality and PA embryo development competence via resisting oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress

Liu

Duan

et al. 2022

Reprod Domestic Animals

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Heat stress (HS) affects the development of porcine gametes and embryos negatively, induces the decrease of reproductive ability significantly, threatens global pig production. Ginsenoside Re (GRe), is a main bioactive component of ginseng, shows very specific anti‐apoptotic, antioxidant and anti‐inflammatory activities. To investigate the alleviating effect of GRe on the in vitro maturation of porcine oocyte under the HS, the polar body extrusion rate, intracellular levels of reactive oxygen species (ROS) and glutathione (GSH), ATP content, mitochondrial membrane potential (MMP) were assessed. For the current study, porcine cumulus‐oocyte complexes (COCs) randomly divided into four groups: the control, GRe, HS and HS + GRe group. The results showed that HS inhibited the cumulus cell expansion and polar body extrusion rate, the levels of GSH and MMP, the ATP content, the gene expression of Nrf2 of porcine oocytes and the parthenogenetic activation (PA) embryo development competence, but GRe treatment could partly neutralize these adverse effects. Furthermore, HS increased the ROS formation and percentage of apoptosis, the gene expression of HSP90, CASP3 and CytoC of porcine oocytes, but GRe could weaken the effect on Cyto C and BAX expression induced by HS. Taken together, these results showed that the presence of GRe during in vitro maturation protects porcine oocytes from HS. These findings lay a foundation for GRe may be used as a potential protective drug to protect porcine oocytes against HS damage.

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“…Cell apoptosis, overproduction of ROS, and reduction of ATP level are closely related [ 40 ]. In recent years, lots of compounds, such as vitexin [ 41 ] and Ginsenoside Re [ 42 ], are discovered to alleviate I/R damage of cardiomyocytes via improving ATP content. Qin et al .…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin-3-gallate protects cardiomyocytes from hypoxia-reoxygenation damage via raising autophagy related 4C expression

et al. 2021

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Myocardial ischemia/reperfusion (I/R) injury is a serious issue during the therapy of myocardial infarction. Herein, we explored the beneficial influence of Epigallocatechin-3-gallate (EGCG) on hypoxia/reoxygenation (H/R)-stimulated cardiomyocyte H9c2 cells damage, along with possible internal molecular mechanism related autophagy related 4C (ATG4C). H9c2 cells were subjected to H/R stimulation and/or EGCG treatment. ATG4C mRNA expression was measured via q-PCR assay. ATG4C overexpression plasmid (OE-ATG4C) was transfected to arise ATG4C level. Cell viability, apoptosis, reactive oxygen species (ROS) production, ATP level were tested via CCK-8 assay, Annexin V-FITC/PI staining, DCFH-DA staining and ATP Assay Kit, respectively. Western blotting was performed to test Cleaved-caspase 3, Cleaved-caspase 9, cytochrome C, and LC3B protein levels. H/R stimulation resulted in H9c2 cell viability loss, promoted cell apoptosis, and ROS overproduction, as well as lowered ATP level in cells. EGCG treatment alleviated H/R-resulted H9c2 cell viability loss, cell apoptosis, ROS overproduction, and reduction of ATP level. Moreover, H/R stimulation reduced the ATG4C expression in H9c2 cells, while EGCG raised the ATG4C expression. Overexpression of ATG4C strengthened the beneficial influence of EGCG on H/R-stimulated H9c2 cell viability, apoptosis and ROS production. Besides, ATG4C overexpression weakened the H/R-stimulated H9c2 cell autophagy via reducing LC3B II/I expression. EGCG exerted beneficial influence on H/R-stimulated cardiomyocytes, which protected cardiomyocytes from H/R-stimulated viability loss, apoptosis, and ROS overproduction via enhancing ATG4C expression.

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Ginsenoside Re Treatment Attenuates Myocardial Hypoxia/Reoxygenation Injury by Inhibiting HIF-1α Ubiquitination

Cited by 15 publications

References 28 publications

Pharmacological Properties of Ginsenoside Re

Pharmacological Properties of Ginsenoside Re

Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress

Epigallocatechin-3-gallate protects cardiomyocytes from hypoxia-reoxygenation damage via raising autophagy related 4C expression

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