Ginsenoside Rb1 protects PC12 cells against β-amyloid-induced cell injury

Abstract

Abstract. excessive accumulation of β-amyloid (aβ) has been proposed as a pivotal event in the pathogenesis of alzheimer's disease. Possible mechanisms underlying aβ-induced neuronal cytotoxicity include oxidative stress and apoptosis. reactive oxygen species (roS) have been proposed to be involved in the apoptotic mechanism of aβ-induced cytotoxicity. Ginsenoside rb1 (Grb1), which is among the key compounds of ginsenoside, found in ginseng, may be a potent scavenger of roS. To examine the potential protective effect of Grb1 in aβ 25-35 -induced cytotoxicity, cells were pre-treated with Grb1 for 24 h, and then aβ [25][26][27][28][29][30][31][32][33][34][35] was added to the medium for an additional 24 h. exposure to aβ led to the accumulation of roS and lipid peroxidation, eventually causing a decrease in the Bcl-2/ Bax ratio, caspase-3 activation, cell apoptosis and cell death. Pre-treatment with Grb1 not only inhibited aβ-induced roS overproduction and lipid peroxidation, but also increased the Bcl-2/Bax ratio and attenuated caspase-3 activation, thereby improving cell survival. Grb1 may therefore act as a roS scavenger, and such antioxidant properties may play a protective role against aβ-induced cell injury. Further exploration of Grb1 antioxidant properties may provide novel therapeutic strategies for the treatment of alzheimer's disease.

Ginsenoside Rb1 inhibits fibrillation and toxicity of alpha-synuclein and disaggregates preformed fibrils

“…Gn Rb1 has been previously shown to exert cell protective effects against the toxicity induced by Aβ 42 (Qian, Han, Hu, & Shi, 2009) and Aβ 25-35 (X. Xie et al, 2010).…”

Effects of ginsenoside Re on LPS-induced inflammatory mediators in BV2 microglial cells

“…Previous studies have reported the development of hydrogen peroxide [26,27], glutamate [28], MPP+ [29] and β-amyloid-induced cell death [30]; however, the mechanism by which LPS leads to microglial toxicity remains obscure. In addition, it is noteworthy that G-Re has a protective effect with a markedly attenuated microglial response of caspase 3 activation (Figure 1).…”

Functional differentiation and spatial-temporal co-expression networks of the NBS-encoding gene family in Jilin ginseng, Panax ginseng C.A. Meyer

“…Studies have shown that ginseng possesses several important biological functions for human health, such as recovery and promotion of vitality, improvement of immune and metabolism systems, regulation of central nervous system, etc. [1, 2]. Ginseng native to the Province of Jilin, China, is often known as Jilin ginseng and estimated to produce 85% and 70% of the ginseng of China and the world, respectively.…”