Ginsenoside�Rb1 prevents steroid‑induced avascular necrosis of the femoral head through the bone morphogenetic�protein‑2 and vascular endothelial growth factor pathway

Ye, Junwu; Wei, Daiqin; Peng, Lin; Chang, Tianmin

doi:10.3892/mmr.2019.10553

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Steroid-induced avascular necrosis of the femoral head (SANFH) is a class of non-traumatic femoral head injury that can trigger osteoclast and bone marrow necrosis due to local blood circulation disorders (Wu et al, 2019). The precise mechanism of SANFH remains unclear and may be associated with various factors such as apoptosis and inflammation (Ye et al, 2019). In vitro experiment has shown that SPS can inhibit caspase-3 activation in the osteoblasts of rats and promote bone formation (Cui et al, 2018).…”

Section: Protective Effect Against Steroid-induced Avascular Necrosis Of the Femoral Headmentioning

confidence: 99%

Extraction, Structures, Bioactivities and Structure-Function Analysis of the Polysaccharides From Safflower (Carthamus tinctorius L.)

Cai

et al. 2021

Front. Pharmacol.

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Safflower (Carthamus tinctorius L.) is a herbal plant with a long history of clinical application worldwide, such as coronary heart disease, hypertension, dysmenorrhea and amenorrhea. It is also extensively used as an important oilseed plant for hundreds of years in some countries, like China, India, Mexico and the United States. Therefore, safflower is believed as a crop with dual values of medicine and economy as well. Safflower polysaccharides (SPS), from the plant, are believed as one of the most important biologically active components with multiple pharmacological properties, including anti-tumor, immune regulation, anti-oxidation, and anti-cerebral ischemia reperfusion injury effects. The polysaccharides, from bee pollen of safflower, named PBPC, also attract the attention of researchers because of their particular origin and bioactivities. Although the extraction, purification, structure and biological activities of SPS and PBPC have been studied for decades, there is not any available review both concerning SPS and PBPC. In this condition, this paper aims to systematically review the research progress in extraction, purification, structural characteristics, and bioactivities of SPS and PBPC, and provide basis for the in-depth study about their structure-bioactivity relationship. It will serve as a methodological outline for further research in fields of new drug discovery and clinical application of SPS or PBPC, and simultaneously remind us of unresolved problems noted in the polysaccharide research.

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Section: Protective Effect Against Steroid-induced Avascular Necrosis Of the Femoral Headmentioning

confidence: 99%

Extraction, Structures, Bioactivities and Structure-Function Analysis of the Polysaccharides From Safflower (Carthamus tinctorius L.)

Cai

et al. 2021

Front. Pharmacol.

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“…SANFH rat model (n 44) was induced by 50 mg/kg DEX injected into the gluteus muscle twice a week for 6 weeks, and 36 rats with successful modeling were selected for the following experiments (Ye et al, 2019). The successful modeling rats were assigned into the SANFH group (modeling rats, n 6), and the remaining rats were administrated with Ato (15 mg/kg/day), (Baranowski et al, 2019) for two weeks after DEX injection.…”

Section: Establishment Of Sanfh Rat Modelmentioning

confidence: 99%

“…RIPA buffer (Beyotime Biotechnology Co., Ltd., Shanghai, China) was used to split the sample for 1 h and samples were centrifuged at 4°C at 12,000 xg for 10 min to collect the supernatant. The inflammatory factors tumor necrosis factor (TNF)-α (H052) and interleukin (IL)-1β (H002), and the oxidative stress indices (malondialdehyde, MDA, A003-1-2; superoxide dismutase, SOD, A001-3-2), catalase (CAT, A007-1-1), ALP (A059-2-2) and OST (H152), and caspase-3 activity were detected using corresponding kits (Beyotime) (Xiao et al, 2018;Ye et al, 2019).…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Atorvastatin Upregulates microRNA-186 and Inhibits the TLR4-Mediated MAPKs/NF-κB Pathway to Relieve Steroid-Induced Avascular Necrosis of the Femoral Head

Er-hai

Huang

2021

Front. Pharmacol.

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Steroid-induced avascular necrosis of the femoral head (SANFH) is caused by the death of active components of the femoral head owing to hormone overdoses. The use of lipid-lowering drugs to prevent SANFH in animals inspired us to identify the mechanisms involving Atorvastatin (Ato) in SANFH. However, it is still not well understood how and to what extent Ato affects SANFH. This study aimed to figure out the efficacy of Ato in SANFH and the underlying molecular mechanisms. After establishment of the SANFH model, histological evaluation, lipid metabolism, inflammatory cytokines, oxidative stress, apoptosis, and autophagy of the femoral head were evaluated. The differentially expressed microRNAs (miRs) after Ato treatment were screened out using microarray analysis. The downstream gene and pathway of miR-186 were predicted and their involvement in SANFH rats was analyzed. OB-6 cells were selected to simulate SANFH in vitro. Cell viability, cell damage, inflammation responses, apoptosis, and autophagy were assessed. Ato alleviated SANFH, inhibited apoptosis, and promoted autophagy. miR-186 was significantly upregulated after Ato treatment. miR-186 targeted TLR4 and inactivated the MAPKs/NF-κB pathway. Inhibition of miR-186 reversed the protection of Ato on SANFH rats, while inhibition of TLR4 restored the protective effect of Ato. Ato reduced apoptosis and promoted autophagy of OB-6 cells by upregulating miR-186 and inhibiting the TLR4/MAPKs/NF-κB pathway. In conclusion, Ato reduced apoptosis and promoted autophagy, thus alleviating SANFH via miR-186 and the TLR4-mediated MAPKs/NF-κB pathway.

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“…Excessive oxidative stress leads to an imbalance between bone resorption and bone formation, thereby leading to loss of bone mineral density [13]. Multiple drugs exerted therapeutic effects on ONFH through their antioxidative properties [14][15][16], highlighting the significance of oxidative stress in the pathogenesis of ONFH.…”

Section: Introductionmentioning

confidence: 99%

Tanshinone I Mitigates Steroid-Induced Osteonecrosis of the Femoral Head and Activates the Nrf2 Signaling Pathway in Rats

Shen

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Steroid-induced osteonecrosis of the femoral head (SIONFH) is a frequent orthopedic disease caused by long-term or high-dose administration of corticosteroids. Tanshinone I (TsI), a flavonoid compound isolated from Salvia miltiorrhiza Bunge, has been reported to inhibit osteoclastic differentiation in vitro. This study aimed to investigate whether TsI can ameliorate SIONFH. Herein, SIONFH was induced by intraperitoneal injection of 20 μg/kg lipopolysaccharide every 24 h for 2 days, followed by an intramuscular injection of 40 mg/kg methylprednisolone every 24 h for 3 days. Four weeks after the final injection of methylprednisolone, the rats were intraperitoneally administrated with low-dose (5 mg/kg) and high-dose (10 mg/kg) TsI once daily for 4 weeks. Results showed that TsI significantly alleviated osteonecrotic lesions of the femoral heads as determined by micro-CT analysis. Furthermore, TsI increased alkaline phosphatase activity and expressions of osteoblastic markers including osteocalcin, type I collagen, osteopontin, and Runt-related transcription factor 2 and decreased tartrate-resistant acid phosphatase activity and expressions of osteoclastic markers including cathepsin K and acid phosphatase 5. TsI also reduced inflammatory response and oxidative stress and activated the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway in the femoral heads. Taken together, our findings show that TsI can relieve SIONFH, indicating that it may be a candidate for preventing SIONFH.

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Ginsenoside�Rb1 prevents steroid‑induced avascular necrosis of the femoral head through the bone morphogenetic�protein‑2 and vascular endothelial growth factor pathway

Cited by 7 publications

References 27 publications

Extraction, Structures, Bioactivities and Structure-Function Analysis of the Polysaccharides From Safflower (Carthamus tinctorius L.)

Extraction, Structures, Bioactivities and Structure-Function Analysis of the Polysaccharides From Safflower (Carthamus tinctorius L.)

Atorvastatin Upregulates microRNA-186 and Inhibits the TLR4-Mediated MAPKs/NF-κB Pathway to Relieve Steroid-Induced Avascular Necrosis of the Femoral Head

Tanshinone I Mitigates Steroid-Induced Osteonecrosis of the Femoral Head and Activates the Nrf2 Signaling Pathway in Rats

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