Ginsenoside Rb1 inhibits proliferation and promotes apoptosis by regulating HMGB1 in uterine fibroid cells

Abstract

To study the effects of ginsenoside Rb1 and the molecular mechanisms on proliferation and apoptosis of uterine fibroid cells, Rb1 þ pc DNA3.1, Rb1 þ pc DNA3.1-HMGB1, si-NC or si-HMGB1 was transfected into uterine fibroid cells by liposome method; the inhibitory rate and proliferation of human uterine fibroid cells were detected by MTT assay; apoptosis of uterine fibroid cells was detected by flow cytometry assay; HMGB1 protein expression in uterine fibroid cells was detected by Western blot assay. Compared with untreated uterine fibroid cells, the inhibitory and apoptosis rate of uterine fibroid cells treated with Rb1 were significantly up-regulated, while the expression level of HMGB1 was significantly down-regulated (p < .05). HMGB1 knockdown inhibited proliferation and promoted apoptosis of uterine fibroid cells. HMGB1 overexpression reversed the inhibitory effect on proliferation and the promotion effect on apoptosis of Rb1 in uterine fibroid cells. Ginsenoside Rb1 could inhibit uterine fibroid cells proliferation and promote apoptosis. This mechanism might be directly related to the downregulation of HMGB1, providing a basis for the treatment of uterine fibroids with ginsenoside Rb1. ARTICLE HISTORY

Influence of Herbal Medicines on HMGB1 Release, SARS-CoV-2 Viral Attachment, Acute Respiratory Failure, and Sepsis. A Literature Review

“…It is likely that Korean ginseng will also reduce HMGB1 levels, because cytokines under the control of NF-kB, such as TNF and IFN-g, induce HMGB1 [104]. In fact, ginsenoside has been shown to decrease HMGB1 levels in a human uterine fibroid cell model [105]. Its direct anti-viral function has also been proven against Influenza strain H1N1, which was prevented from attaching to host α 2-3' sialic acid receptors by ginsenoside interaction with viral Haemagglutinin when administered topically to the viral infection site intranasally [106].…”

Enhancing effect of borneol on pharmacokinetics of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1 in healthy volunteers after oral administration of compound Danshen dropping pills

“…Ginsenosides, such as Rb 1 and Rg 1 , a group of triterpenoid saponins, are also widely used to treat diseases of the nervous system [Alzheimer's disease (Lin et al, 2019), Parkinson's disease (Zhang et al, 2018), and cerebral ischemia (Dong et al, 2017; Yan et al, 2018)], tumors (Zhang et al, 2019), and diabetes and their complications (Shao et al, 2019; P. Zhou, Xie, et al, 2019). However, numerous studies have found that Rb 1 and Rg 1 exhibit lower oral bioavailability in vivo (Peng et al, 2012; Zhou, Wu, et al, 2019).…”