Ginsenoside production in different phenotypes of Panax ginseng transformed roots

Mallol, Anna; Cusidó, Rosa M.; Palazón, Javier; Bonfill, Mercedes; Morales, C; Piñol, M. Teresa

doi:10.1016/s0031-9422(01)00062-0

Cited by 85 publications

(49 citation statements)

References 25 publications

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“…Agrobacterium rhizogenes A4 induced a thin root morphology with a low degree of branching similar to that described for P. ginseng (Mallol et al 2001). On the other hand,…”

Section: Resultssupporting

confidence: 59%

“…Within the hairy root morphology of C. roseus, three separate types of morphology could be distinguished: less branched and slow-growing, highly branched and moderately-growing, profusely branched and fast-growing Shanks, 1995, Batra et al 2004). A third type of morphology, thin without branching, was also observed (Mallol et al 2001). The root morphology developed by hairy root cultures induced in the three species of Echinacea depended on the Agrobacterium strain used.…”

Section: Resultsmentioning

confidence: 86%

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Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Orellana¹,

Ferri²

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“…Agrobacterium rhizogenes A4 induced a thin root morphology with a low degree of branching similar to that described for P. ginseng (Mallol et al 2001). On the other hand,…”

Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 86%

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Orellana¹,

Ferri²

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“…On the other hand, in hemp hairy roots showed higher growth rate (Table 5), branching and root hair density (Figure 2g, 2h), but no correlation between ethylene biosynthesis and root morphology (Table 5) was observed. Finally, the dedifferentiation ability leading to the so-called callus-like morphology described for hairy roots of some Solanaceae plants (Moyano et al 1999), Pennax gingsen (Mallol et al 2001), Withania somnifera (Bandyopadhyay et al 2007), and Gentiana macrophilla (Tiwari et al 2007), have not been observed in this work. It is well known that T-DNA-rol genes of Ri plasmid are responsible for hairy root induction in many plants.…”

Section: Discussionmentioning

confidence: 59%

Agrobacteriuminfection of hemp (Cannabis sativaL.): establishment of hairy root cultures

Wahby

Caba

Ligero

2013

Journal of Plant Interactions

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Experimental conditions were optimized for hemp, a difficult to transform plant, to be effectively infected with either Ri or Ti plasmid-bearing agrobacteria and to establish stably transformed tissues. Hypocotyl of intact seedlings was the most responsive material and the response depended on both bacterial strain and plant variety. Transformed tissues, hairy roots and tumors, were cultured and stabilized in vitro and showed the characteristic traits of fast and phytohormone-independent growth as well as high incidence of lateral branching and abundance of root hairs in the case of roots. They all contained T-DNA of the corresponding Ri or Ti plasmid as revealed by PCR analysis with specific primers and further hairy roots induced by AR10GUS strain showed normal pattern of b-glucuronidase positive staining. To our knowledge, this represents the first reported protocol for the establishment of Cannabis sativa hairy root cultures.

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“…Hypothetically, the synthesis of this compound was powerfully infl uenced by high auxin level present in the transformed roots. The genetic transformation mediated by 15834 A. rhizogenes strain promotes endogenous auxin level increment because aux genes are introduced in the plant genome (Mallol et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Marques¹,

Foglio²,

Morgante³

et al. 2006

Rev. bras. farmacogn.

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RESUMO: "Ferramentas biotecnológicas para a produção de dihidro-epideoxiarteanuina B, um antiulcerogênico isolado de Artemisia annua L.". Foram desenvolvidas metodologias para o estabelecimento e cultivo de raízes de Artemisia annua L. (híbrido CPQBA 2/39 x PL5). Estas raízes foram submetidas a diferentes condições de luz e a transformação genética com Agrobacterium rhizogenes (cepas 8196 e 15834). As raízes transgênicas e não-transgênicas (normais) foram cultivadas em meios de Murashige e Skoog (1962), mantidas sobre diferentes condições de fotoperíodo e analisadas para avaliação do conteúdo do composto antiulcerogênico dehidro-epideoxiarteanuína B (composto A). A confi rmação do caráter transgênico das raízes foi obtida por Dot Blot. Os extratos dos materiais vegetais foram analisados por Cromatografi a Gasosa acoplada a um Espectômetro de Massas (CG/EM). Os cromatogramas dos extratos das raízes normais revelaram a presença de dehidro-epideoxiarteanuína B e de um outro composto (composto B). As condições fotoperiódicas de cultivo infl uenciaram na produção destes dois compostos, sendo que sobre condição de escuro contínuo, dehidro-epideoxiarteanuína B foi intensamente produzido e o composto B foi detectado em pequenas proporções, enquanto que sob fotoperíodo de 16 horas, o inverso ocorreu. A quantifi cação de dehidro-epideoxiarteanuína B por Cromatografi a Gasosa acoplada a um Detector de Ionização de Chamas (CG/FID) revelou um aumento de aproximadamente cinco vezes na produção deste composto pelas raízes normais cultivadas sobre escuro contínuo em relação às raízes cultivadas na presença de 16 horas de luz. O terpeno dehidro-epideoxiarteanuína B não estava presente nas raízes transgênicas.Unitermos: Artemisia annua, terpeno, cultura de raízes in vitro, raízes transgênicas, luz.ABSTRACT: Methodologies were developed for the establishment and cultivation of Artemisia annua L (CPQBA 2/39 x PL5 hybrid) roots submitted to light conditions and genetic transformation performed with Agrobacterium rhizogenes (15834 and 8196 strains). The transgenic and nontransgenic (normal) roots were cultured in Murashige and Skoog (1962) medium, kept under different photoperiodic conditions and analyzed for evaluation of the antiulcerogenic dihydroepideoxyarteannuin B (compound A) contents. The Dot Blot technique was used to confi rm the transgenic nature of the roots. The plants′s crude extracts were analyzed by Gas Chromatography coupled to Mass Spectrum (CG/MS). The chromatograms of the extracts taken from normal roots revealed the presence of dihydro-epideoxyarteannuin B and other compound (compound B). Photoperiods during cultivation infl uenced the production of these two compounds: under continuous darkness dihydro-epideoxyarteannuin B was intensely produced and the compound B present in small amounts, while on 16 h photoperiod, the inverse occurred. The quantifi cation of dihydro-epideoxyarteannuin B by Gas Chromatography coupled to Flame Detector Ionization (CG/ FID) revealed an approximately fi vefold increase in the prod...

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Ginsenoside production in different phenotypes of Panax ginseng transformed roots

Cited by 85 publications

References 25 publications

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

Agrobacteriuminfection of hemp (Cannabis sativaL.): establishment of hairy root cultures

Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

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