Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil

Wang, Chong‐Zhi; Hou, Lifei; Wan, Jianwei; Yao, Haiqiang; Jie, Yuan; Zeng, Jinxiang; Park, Chan Woong; Kim, Su Hwan; Seo, Dae Bang; Shin, Kwang‐Soon; Zhang, Chunfeng; Chen, Lina; Zhang, Qi‐Hui; Li, Zhi; Sava-Segal, Clara; Yuan, Chun‐Su

doi:10.1016/j.jgr.2018.12.010

Cited by 35 publications

(27 citation statements)

References 42 publications

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“…ere were many studies which combine natural compounds with 5-FU to enhance anti-proliferative activity and reduce 5-FU doses. Previous study reported the combination of 5-FU with Asian ginseng berry polysaccharide could reduce 5-FU doses and increase the anti-proliferative activity of colorectal cancer significantly [22].…”

Section: Gene Expressionmentioning

confidence: 96%

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Laila

Fardiaz

Yuliana

et al. 2020

International Journal of Food Science

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Coleus amboinicus(Lour) (CA) has been reported to possess many pharmacological activities. In this study, evaluation of cytotoxicity using brine shrimp lethality bioassay and MTT assay using WiDr cell lines was carried out. The expression of several genes responsible for programmed cell death of the methanol extract of CA was also investigated. The morphology of the cells undergoing apoptosis was detected using Hoechst staining assay. The gene expression of BAX, BCL2, P53, Caspase 1, 7, 8, and 9 of treated samples with different concentrations (10, 15, 25 & 50 µg/ml) were measured with RT PCR. The phytochemical profiles were investigated using LC MS. The results showed that the lethality concentration (LC50) of methanol extract using brine shrimp was 34.545 µg/ml and the extract exhibited good antiproliferative activity against cancer cells WiDr with IC50 value (8.598 ± 2.68 µg/ml) as compared to standard drug 5-fluorouracil (IC50 value 1.839 ± 0.03 µg/ml). There was apoptotic evidences from the morphology of treated cells. The expressions of BAX,P53, and Caspase 9 were upregulated in lower concentration of the extract (10 and 15 µg/ml) but downregulated in higher concentration (25 and 50 µg/ml). BCL2 as anti-apoptotic gene was downregulated in all concentrations. Caspase 1 and Caspase 7 were upregulated in high concentration (25 and 50 µg/ml), but downregulated in lower concentrations. These data provide a mode of cell death for the methanol extract of CA in low concentrations corresponding to apoptosis with intrinsic pathway. Many valuable compounds identified including caffeic acid, rosmarinic acid, malic acid, eicosapentanoic acid, benserazide, alpha-linolenic acid, betaine, Salvanolic B, 4-hydroxibenzoic acid and firulic acid have been previously reported as being active agents against many cancer cells. This study suggested that CA might become an effective ingredient for health-beneficial foods to prevent colon cancer.

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Section: Gene Expressionmentioning

confidence: 96%

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Laila

Fardiaz

Yuliana

et al. 2020

International Journal of Food Science

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“…The design concept of amino acid uorescent probe is that considering the error of detection results caused by the toxicity of the probe itself, pyrimidine [32][33][34], which is widely used in the drug eld, is selected as the intermediate of targeting action to introduce the uorescent group BODIPY [35]. This design not only greatly reduces the toxicity problem of the probe, but also has certain drug activity in the overall structure.…”

Section: Resultsmentioning

confidence: 99%

Study On The Responsiveness of A Novel Pyrimidine-BODIPY Probe For Rapid Identification of Different Amino Acids

Jin¹,

Shao²,

Jin³

2021

Preprint

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Rapid identification of different amino acid responses is of great importance in the field of biomedicine. Through the combination of the drug intermediate pyrimidine with the fluorophore BODIPY, a new type of high-efficiency fluorescent probe for rapid identification of different amino acids was prepared. In terms of photophysical properties, the UV absorption and fluorescence emission of the mercapto groups were observed under the conditions of different hydroperoxide equivalents and different solvents, and the maximum values were in the range of 534–538 nm and 554–560 nm. When different amino acids were added, the UV absorption and fluorescence emission ranges were 538 ~ 540 nm and 560 ~ 564 nm. Finally, the identification sensitivity of amino acids with different equivalents under different oxidant conditions was quickly detected by fluorescence cuvette. Among them, the identification response to tyrosine was the most obvious.

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“…It can also inhibit the lung metastatic activity of B16-BL6 melanoma cells (Lee et al, 2019a;Lee et al, 2019b). In addition, GBP can significantly inhibit the differentiation of Th1 cells and the differentiation of Treg cells (hindering the body's immune response to malignant tumors), and the differentiation of Treg cells Down-regulation may help the anti-cancer potential of GBP and reshape the tumor microenvironment (Wang C.-Z. et al, 2020).…”

Section: Ginseng Polysaccharidesmentioning

confidence: 99%

Natural Polysaccharides and Their Derivates: A Promising Natural Adjuvant for Tumor Immunotherapy

Wang

et al. 2021

Front. Pharmacol.

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The treatment process of tumor is advanced with the development of immunotherapy. In clinical experience, immunotherapy has achieved very significant results. However, the application of immunotherapy is limited by a variety of immune microenvironment. For a long time in the past, polysaccharides such as lentinan and Ganoderma lucidum glycopeptide have been used in clinic as adjuvant drugs to widely improve the immunity of the body. However, their mechanism in tumor immunotherapy has not been deeply discussed. Studies have shown that natural polysaccharides can stimulate innate immunity by activating upstream immune cells so as to regulate adaptive immune pathways such as T cells and improve the effect of immunotherapy, suggesting that polysaccharides also have a promising future in cancer therapy. This review systematically discusses that polysaccharides can directly or indirectly activate macrophages, dendritic cells, natural killer cells etc., binding to their surface receptors, inducing PI3K/Akt, mitogen-activated protein kinase, Notch and other pathways, promote their proliferation and differentiation, increasing the secretion of cytokines, and improve the state of immune suppression. These results provide relevant basis for guiding polysaccharide to be used as adjuvants of cancer immunotherapy.

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Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil

Cited by 35 publications

References 42 publications

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Study On The Responsiveness of A Novel Pyrimidine-BODIPY Probe For Rapid Identification of Different Amino Acids

Natural Polysaccharides and Their Derivates: A Promising Natural Adjuvant for Tumor Immunotherapy

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