Ginkgolide C reduced oleic acid-induced lipid accumulation in HepG2 cells

Abstract

Ginkgolide C, isolated from Ginkgo biloba, is a diterpene lactone that has multiple biological functions and can improve Alzheimer disease and platelet aggregation. Ginkgolide C also inhibits adipogenesis in 3T3-L1 adipocytes. The present study evaluated whether ginkgolide C reduced lipid accumulation and regulated the molecular mechanism of lipogenesis in oleic acid-induced HepG2 hepatocytes. HepG2 cells were treated with 0.5 mM oleic acid for 48 h to induce a fatty liver cell model. Then, the cells were exposed to various concentrations of ginkgolide C for 24 h. Staining with Oil Red O and the fluorescent dye BODIPY 493/503 revealed that ginkgolide C significantly reduced excessive lipid accumulation in HepG2 cells. Ginkgolide C decreased peroxisome proliferator-activated receptor γ and sterol regulatory element-binding protein 1c to block the expression of fatty acid synthase. Ginkgolide C treatment also promoted the expression of adipose triglyceride lipase and the phosphorylation level of hormone-sensitive lipase to enhance the decomposition of triglycerides. In addition, ginkgolide C stimulated CPT-1 to activate fatty acid β-oxidation, significantly increased sirt1 and phosphorylation of AMP-activated protein kinase (AMPK), and decreased expression of acetyl-CoA carboxylase for suppressed fatty acid synthesis in hepatocytes. Taken together, our results suggest that ginkgolide C reduced lipid accumulation and increased lipolysis through the sirt1/AMPK pathway in oleic acid-induced fatty liver cells.

Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety

“…Moreover, Ginkgolide C stimulated the production of adipose triglyceride lipase and hormone-sensitive lipase, leading to elevated lipolysis levels (Liou et al, 2015). Similar results were obtained with human HepG2 hepatocyte cell line (Huang et al, 2018).…”

“…VSMC, vascular smooth muscle cell; ECM, extracellular matrix; EC, endothelial cell; NO, nitric oxide; PPARY, peroxisome proliferator-activated receptor-gamma. By decreasing VEGF-A and FGF-2 levels (Kim et al, 1999b;Keum et al, 2003;Park et al, 2005;Persson et al, 2006;Shin Y. et al, 2013;Deng et al, 2017;Singh et al, 2017;Lee et al, 2019a) Gingko By decreasing caspase 3 and pro-apoptotic Bax expression and increasing anti-apoptotic Bcl-2 expression (Akiba et al, 2007;Lin et al, 2007;Mansour et al, 2011;Liu et al, 2013;Liou et al, 2015;Mesquita et al, 2017;Abdel-Zaher et al, 2018;Huang et al, 2018;Chen et al, 2019).…”

“…In addition, vasodilatory and antihypertensive properties of GBE can exert cardioprotective benefits (Perez-Vizcaino et al, 2009). In this regard, GBE has exhibited ACE inhibitory activities (Mansour et al, 2011), activation of cholinergic pathways, endothelial health improvement, inhibition of endothelium activation and adhesion (Mesquita et al, 2017), and serum lipid-lowering activities (Liou et al, 2015;Huang et al, 2018) among other reported effects that are beneficial in CVD.…”

Tri-Herbal Medicine Divya Sarva-Kalp-Kwath (Livogrit) Regulates Fatty Acid-Induced Steatosis in Human HepG2 Cells through Inhibition of Intracellular Triglycerides and Extracellular Glycerol Levels

“…A similar protective response of SKK was earlier observed by us in HepG2 cells treated with carbon tetrachloride [ 19 ]. Plant metabolites comprising of rotenoids, catechin, gallic acid, quercetin, rutin, corilagin, and caffeic acid play a major role in the hepatoprotective role of SKK against a wide range of liver ailments [ 19 , 35 , 36 , 37 , 38 ].…”

Synthesis and Characterization of Novel Resveratrol Butyrate Esters That Have the Ability to Prevent Fat Accumulation in a Liver Cell Culture Model

“…GAPDH was used as a loading control. Finally, the membrane was incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000) at room temperature for 1 h. After three washes, the chemiluminescence signals were developed using an ECL (Enhanced Chemiluminescent) detection kit (PerkinElmer, Shelton, CT, USA), and the density of bands was counted using ImageJ gel analysis software [ 50 ].…”