Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage

Silva, Amélia M.; Silva, Sandra C.; Soares, Jorge Pinto; Martins-Gomes, Carlos; Teixeira, João Paulo; Leal, Francisco J. Vaz; Gaivão, Isabel

doi:10.3390/plants8120556

Cited by 16 publications

(16 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the protective properties of other plant extracts rich in polyphenols are well known. Silva et al [55] reported that co-incubation of HepG2 cells for 4 h with Gingko biloba extract (75 µg/mL) and paraquat (1.0 or 1.5 µM) significantly reduced paraquat-induced oxidative DNA damage. Zakłos-Szyda et al [53] found that phenolic extract from Viburnum opulus fruits induced DNA repair after 120 min of pretreatment with H 2 O 2 or methylnitronitrosoguanidine, reaching 93% and 88%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Efenberger-Szmechtyk

Nowak

2020

Antioxidants

View full text Add to dashboard Cite

Aronia melanocarpa, Cornus mas, and Chaenomeles superba leaf extracts contain large amounts of bioactive compounds—mainly polyphenols, which possess many health benefits including anti-cancer properties. Here, we investigate the biological effects of A. melanocarpa, C. mas, and C. superba leaf extracts on the human colon adenocarcinoma cell line Caco-2. The antiproliferative activity of the extracts was measured using the MTT assay. The most cytotoxic extract was C. mas (IC50 = 0.60%). The extracts caused morphological changes in the Caco-2 cells, including partial detachment of cells, necrotic cells, chromatin condensation, cytoplasmic vacuolization, cell nuclei lysis, and nucleus fragmentation. The DNA damage in the Caco-2 cells after exposure to the leaf extracts was measured using the alkaline comet assay. The extracts increased DNA damage in a concentration dependent manner. However, at lower non-cyto- and non-genotoxic (IC0) concentrations the extracts induced DNA repair in Caco-2 cells after exposure to hydrogen peroxide. In conclusion, the results of these studies suggest that A. melanocarpa, C. mas and C. superba leaf extracts can show anticancer activity. However, further research is required on the mechanisms of anti-cancer activity by these extracts, with the application of more advanced and wide-ranging techniques including in vivo experiments.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Efenberger-Szmechtyk

Nowak

2020

Antioxidants

View full text Add to dashboard Cite

show abstract

“…Another example is paraquat-induced toxicity, which has been widely described (e.g., [ 16 , 17 ]). Although presenting slow skin and oral absorption, its bioaccumulation in human organs, namely in lung or in kidney, has been shown to induce severe organ damage [ 18 , 19 ], and contact with the pesticide can induced skin irritation, nausea, diarrhea and abdominal pain [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Glyphosate vs. Glyphosate-Based Herbicides Exposure: A Review on Their Toxicity

Martins-Gomes

Silva

Andreani

et al. 2022

JoX

Self Cite

View full text Add to dashboard Cite

Glyphosate-based herbicide has been the first choice for weed management worldwide since the 1970s, mainly due to its efficacy and reported low toxicity, which contributed to its high acceptance. Many of the recent studies focus solely on the persistence of pesticides in soils, air, water or food products, or even on the degree of exposure of animals, since their potential hazards to human health have raised concerns. Given the unaware exposure of the general population to pesticides, and the absence of a significant number of studies on occupational hazards, new glyphosate-induced toxicity data obtained for both residual and acute doses should be analyzed and systematized. Additionally, recent studies also highlight the persistence and toxicity of both glyphosate metabolites and surfactants present in herbicide formulations. To renew or ban the use of glyphosate, recently published studies must be taken into account, aiming to define new levels of safety for exposure to herbicide, its metabolites, and the toxic excipients of its formulations. This review aims to provide an overview of recent publications (2010–present) on in vitro and in vivo studies aimed at verifying the animal toxicity induced by glyphosate, its metabolite aminomethylphosphonic acid (AMPA) and glyphosate-based formulations, evaluated in various experimental models. Apart from glyphosate-induced toxicity, recent data concerning the role of surfactants in the toxicity of glyphosate-based formulations are discussed.

show abstract

“…The increasing interest in phenolic compounds as natural ingredients for food additives and as health promoters resulted in a deeper investigation of many plant species that are widely used in folk medicine (e.g., [1][2][3]). Ethnobotanical surveys, particularly from the Mediterranean area, often describe the medicinal uses of plants from the genus Thymus, a member of the Lamiaceae family, due to their high anti-microbial [4], anti-oxidant [5,6], anti-inflammatory [7][8][9], and anti-proliferative [6,[10][11][12] activities.…”

Section: Introductionmentioning

confidence: 99%

Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

et al. 2019

Self Cite

View full text Add to dashboard Cite

Thymus mastichina, also called mastic thyme or Spanish marjoram, is endemic to the Iberian Peninsula, where it is widely used in folk medicine especially for treating digestive and respiratory systems disorders, and as a condiment to season olives. This work describes for the first time the detailed phenolic composition of exhaustive hydroethanolic extracts and aqueous decoctions of Thymus mastichina. Unlike other species of the Thymus genera, Thymus mastichina extracts contain high amounts of salvianolic acid derivatives, with salvianolic acid A isomer being the main derivative. This isomer was identified in extracts from Thymus mastichina for the first time. Also, an undescribed salvianolic acid derivative in Thymus mastichina was identified and its structure was tentatively described. Extracts from Thymus mastichina showed significant scavenging activity of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical cation, hydroxyl, and nitric oxide radicals. The anti-proliferative effect of both T. mastichina extracts were tested against Caco-2 and HepG2 cells; the hydroethanolic extract showed a high anti-proliferative activity against Caco-2 cells compared to HepG2 cells (at 24 h exposure, the concentration that inhibits 50% of proliferation, IC50, was 71.18 ± 1.05 µg/mL and 264.60 ± 11.78 µg/mL for Caco-2 and HepG2, respectively). Thus, these results make this species a promising candidate for further investigation of its anti-tumoral potential. Therefore, Thymus mastichina can be potentially used as a functional food (used as a decoction or herbal tea) or as a source of bioactive ingredients with antioxidant and anti-proliferative properties.

show abstract

Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage

Cited by 16 publications

References 49 publications

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Glyphosate vs. Glyphosate-Based Herbicides Exposure: A Review on Their Toxicity

Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

Contact Info

Product

Resources

About