Gingival crevicular fluid levels of calprotectin and myeloperoxidase during therapy for generalized aggressive periodontitis

Kaner, Doğan; Bernimoulin, Jean‐Pierre; Kleber, Bernd‐Michael; Heizmann, W.; Friedmann, Anton

doi:10.1111/j.1600-0765.2005.00849.x

Cited by 62 publications

(79 citation statements)

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“…Our findings are consistent with the previous data showing the role of calprotectin as an inflammatory marker of periodontal disease [11,12,42,43]. Kaner et al [44] have demonstrated that GCF calprotectin levels are significantly correlated with the levels of periodontal inflammation and decreased after periodontal therapy in G-AgP patients. Elevated GCF calprotectin levels in diseased groups might be due to the increased activation of inflammatory cells by bacterial components as well as greater release of intracellular material in gingivitis and periodontitis [45][46][47].…”

Section: Discussionsupporting

confidence: 83%

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

et al. 2011

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Abstract. Aim:The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases. Material and methods: Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p < 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p < 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p < 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p < 0.008). Conclusions: Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.

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Section: Discussionsupporting

confidence: 83%

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

et al. 2011

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“…Although MPO is involved in the pathogenesis of infl ammatory periodontal diseases and pancreati c cancer, it is also found in clinically healthy sites in lower levels than the periodentally diseases sites 9,13,14 .…”

Section: Resultsmentioning

confidence: 99%

“…GCF sampling and processing were done as has been described in detail elsewhere 12 . MPO acti vity (GM) is analysed as previous studies 13 . Relati onships between GM; probing depths and bleeding on probing, were analysed using a performed during SPSS (version 11.0, Chicago, USA).…”

Section: Rai -Gingival Crevicular Fluid Myeloperoxidase In Periodontimentioning

confidence: 99%

“…It has been also observed that increased myeloperoxidase which is present in azurophilic granules of polymorphonuclear neutrophils, acti vity in periodonti ti s as compared to controls 11 . Myeloperoxidase considered a promising marker of periodontal infl ammati on 12,13 . Hence, the present study was planned to determine the relati onship between GCF myeloperoxidase (GM) levels in periodonti ti s and pancreati c cancer.…”

Section: Rai -Gingival Crevicular Fluid Myeloperoxidase In Periodontimentioning

confidence: 99%

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Gingival Crevicular Fluid Myeloperoxidase in Periodontitis and Pancreatic Cancer

Rai¹

2010

Pesq Bras Odontoped Clin Integr

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“…A MPO tem sido apresentada como um marcador da DP no GCF (Kaner, Bernimoulin et al 2006;Marcaccini, Meschiari et al 2010), porém ainda é pequeno número de trabalhos que mostram a MPO na STE e sua relação com a DP (Sakamoto, Fujii et al 2008). …”

Section: Doença Periodontalunclassified

Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

Meschiari¹

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Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases known to cleave components of the connective tissue in physiological and pathological processes. The regulation of MMP activities is done by the tissue inhibitors of metalloproteinases (TIMPs).Periodontal disease (PD) is a chronic inflammation with excessive activity of MMPs, which degrade the tooth supporting tissues.The saliva components are derived from the local blood supply, and this fluid may therefore reflect the plasma. So, the objectives of this study were: 1) to compare the levels of MMPs, TIMPs and MPO in stimulated whole saliva (SWS) and plasma of PD patients before (PB) and 3 months after (PT) the non-surgical periodontal treatment, and in healthy volunteers at baseline (CB) and 3 months after baseline (CT), and 2) to evaluate the correlations between the results found.Measurements of MMP-8, MMP-9, TIMP-1 and TIMP-2 were performed by ELISA. Gelatinolitic activity of MMP-9 forms were determined by zymography, and the MPO activity was determined by colorimetric assay.There was lower gelatinolitic activity, and lower concentrations of MMP-8 and TIMP-2 in STE on PT group compared with PB group (p <0.05). MPO activity was higher in PB compared to CB (p <0.05).Statistically significant moderate correlations were observed in all associations between MMP-9 performed by ELISA in plasma and gelatinolitic activity bands of STE: MMP-9 molecular form of 92 kDa (r = 0,37, p = 0,0017), MMP-9 molecular form of 130 kDa (r = 0,35, p = 0,003), the sum of its gelatinase activity (130 +92 kDa) (r = 0,43, p = 0,0002), gelatinase of 180 kDa (r = 0,35, p = 0,003), and the sum of total gelatinase activity (180+130+92 kDa) (r = 0,37, p = 0,002). Circulating MMP-8 levels correlate with salivary gelatinase activity bands of 92 kDa (r = 0,30, p = 0,01) and the sum of gelatinase activity (130 +92 kDa) (r = 0,24, p = 0,04). In addition, a weak correlation between gelatinase activity of plasmatic 92 kDa MMP-9 and gelatinase of 180 kDa in SWS (r= 0,26 p = 0,03) was found, and a moderate correlation between plasmatic TIMP-2 and TIMP-2 of SWS (r = 0,32, p = 0,004).The results show that the gelatinase activity of SWS may reflect the concentrations of systemic inflammatory markers such as MMP-8 and MMP-9, also the concentration of TIMP-2 in the SWS may reflect the circulating concentration of TIMP-2. The evaluation of these results suggests that there is an attenuation of some inflammatory markers analyzed in the SWS and in plasma after treatment of PD. Furthermore, there are correlations between salivary and plasma levels in some of these markers. Because saliva sampling is less invasive, more studies about the correlations between these markers in these two fluids are necessary. Key words: saliva, plasma, matrix metalloproteinases, periodontal disease. LISTA DE ABREVIATURAS, SIGLAS E SÍMBOLOS BSA -

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Gingival crevicular fluid levels of calprotectin and myeloperoxidase during therapy for generalized aggressive periodontitis

Cited by 62 publications

References 42 publications

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

Gingival Crevicular Fluid Myeloperoxidase in Periodontitis and Pancreatic Cancer

Concentrações de MMP-8, MMP-9, MPO, TIMP-1 e TIMP-2 na saliva e correlações com plasma

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