Ginger extract protects rat’s kidneys against oxidative damage after chronic ethanol administration

Shirpoor, Alireza; Rezaei, Farzaneh; Fard, Amin Abdollahzade; Afshari, Ali; Gharalari, Farzaneh Hosseini; Rasmi, Yousef

doi:10.1016/j.biopha.2016.09.097

Cited by 24 publications

(26 citation statements)

References 39 publications

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“…In the present study, 8‐OHdG levels, which is a biomarker of DNA oxidative damage, significantly changed by indomethacin. However, from recently postulated point of view, the important roles of oxidative DNA damage in kidney injury lacks from the influence of indomethacin on 8‐OHdG generation that clearly pointed out in the present study . In our study, oleuropein suppressed the apoptotic indices as evidenced from 8‐OHdG levels and histopathological studies.…”

Section: Discussionsupporting

confidence: 42%

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

Koç

Çeriğ

Özek

et al. 2018

Environmental Toxicology

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Indomethacin is generally used in clinical therapeutics as a non-steroidal anti-inflammatory drug.However, its use has been limited due to the gastrointestinal and renal toxic effects of this drug.These toxic effects were associated with not only the inhibition of prostaglandin synthesis but also drug-elevated oxidative stress. To ameliorate these toxicities, natural antioxidants can be used as an alternative and/or combination therapies. Therefore, the current study was conducted to assess the renoprotective effects of oleuropein against indomethacin-induced renal damages. Male Sprague-Dawley rats were pretreated with oleuropein (75, 150, and 300 mg/kg), and then treated with indomethacin (25 mg/kg). To evaluate kidney function, serum blood urea nitrogen, uric acid, and creatinine were measured. In addition, prostaglandin E 2 , tumor necrosis factor-alpha, endothelial nitric oxide synthase, caspase-3, oxidant/antioxidant status, and 8-Oxo-2 0 -deoxyguanosine levels were determined for the antioxidative and anti-inflammatory effects of oleuropein. Tissue sections were also histopathologically assessed. The biochemical and histopathological analysis proved the toxic effects of indomethacin on kidney. However, the pretreatment with oleuropein (300 mg/kg) protects kidney from indomethacin-induced damages. Our study proved that prior administration of oleuropein has renoprotective activity against indomethacin-associated toxicities. K E Y W O R D S8-OHdG, eNOS, histopathologic assessment, oleuropein, prostaglandin E2

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Section: Discussionsupporting

confidence: 42%

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

Koç

Çeriğ

Özek

et al. 2018

Environmental Toxicology

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“…Moreover, the current findings showed that chronic ethanol exposure caused oxidative stress in the epididymis tissue, as indicated by an increase in NADPH‐oxidase and 8‐OHdG levels. Our previous laboratory studies (Shirpoor, Barmaki, Khadem Ansari, Lkhanizadeh, & Barmaki, ; Shirpoor et al, ; Shirpoor, Nemati, Ansari, & Ilkhanizadeh, ; Shirpoor, Zerehpoosh, Ansari, Kheradmand, & Rasmi, ), as well as other similar studies in the literature (Abarikwu et al, ; Teixeira et al, ), have revealed that chronic ethanol consumption in male rats leads to oxidative stress and inflammatory changes in the testis, heart, lung, and kidney.…”

Section: Discussionmentioning

confidence: 65%

“…For histopathological staining, histological sections (thickness, 5 µm) were cut from the paraffin‐embedded epididymis tissue and stained with hematoxylin and eosin (H&E). As described in our previous study, cell proliferation was examined by immunohistochemistry, using an antibody against the PCNA (Shirpoor et al, ). Briefly, tissue sections (thickness, 5 μm) were obtained from formalin‐fixed specimens and stained with monoclonal anti‐PCNA antibody (Dako Denmark A/S, Denmark).…”

Section: Methodsmentioning

confidence: 99%

“…The number of positively stained cells and the total number of cells were counted for each field. The percentage of positively stained cells to the total number of counted cells was classified as follows: normal, PCNA‐positive markers are present in less than 5% of epididymis cells; mild, PCNA‐positive markers are present in less than 25% of epididymis cells; mild to moderate, PCNA‐positive markers are present in 25–50% of epididymis cells; moderate to severe, PCNA‐positive markers are present in 50–75% of epididymis cells; and severe, PCNA‐positive markers are present in 75–100% of epididymis cells (Shirpoor et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Long‐term ethanol consumption promotes changes in β‐defensin isoform gene expression and induces structural changes and oxidative DNA damage to the epididymis of rats

Sadeghzadeh

Shirpoor

Naderi

et al. 2019

Molecular Reproduction Devel

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Chronic alcohol ingestion causes sexual dysfunction, impairs sperm motility and fertility, and changes semen quality. Considering the key role of epididymis in sperm development, the aim of the present study was to evaluate the effects of long‐term ethanol consumption on epididymis changes, including alterations in β‐defensin isoform gene expression, oxidative stress, and pathological changes, such as cell proliferation and fibrosis in the epididymis of rats. In this study, male Wistar rats were equally divided into control and ethanol (4.5 g/kg BW) groups. After six weeks of treatment, the results revealed the proliferation of epididymis cells, fibrosis in the epididymis tissue, and a significant rise in the level of 8‐OHdG and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in the ethanol group, compared with the control group. Moreover, the ethanol group showed an increase in the gene expression of epididymal β‐defensin isoforms 15 and 21 and a reduction in the gene expression of β‐defensin isoforms 27 and 30, compared with the controls. These findings indicate that ethanol‐induced epididymal damage and sperm abnormalities might be partly associated with changes in β‐defensin isoforms and epididymal structure, mediated by the increased activities of 8‐OHdG and NADPH oxidase.

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“…The glomerular filtration rate (GFR) was estimated by calculating creatinine clearance. This was done using 24 h urine volume as well as serum and urine concentrations of creatinine via the formula ( Shirpoor et al., 2016 ; Oosterhuis et al., 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Smilax glabra Roxb. Against Lead-Induced Renal Oxidative Stress, Inflammation and Apoptosis in Weaning Rats and HEK-293 Cells

Shi

Tian

et al. 2020

Front. Pharmacol.

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Lead (Pb) is an important environmental pollutant. Oxidative stress and the inflammatory response have been postulated as mechanisms involved in lead-induced renal damage. Smilax glabra Roxb. has been used for treatment of heavy-metal poisoning in China for 500 years. We investigated S. glabra flavonoids extract (SGF) could attenuate lead acetate-induced nephrotoxicity in weaning rats and human embryonic kidney (HEK)-293 cells, and investigated the possible mechanisms. Compared with Pb exposed group of weaning rats, SGF could significantly promote lead excretion in the blood and kidney, and increase the content of the renal-function indicators blood urea nitrogen, serum uric acid, and serum creatinine. SGF could improve the glomerular filtration rate (GFR) and histologic changes in the kidneys of weaning rats exposed to Pb. SGF could also reduce lead-induced cytotoxicity, improve DNA damage-induced apoptosis and cleaved caspase-3-mediated apoptosis in HEK-293 cells stimulated with Pb. SGF significantly increased the activity of the antioxidant enzymes superoxide dismutase, glutathione peroxidase and catalase, and decreased excessive release of reactive oxygen species (ROS) and malondialdehyde in the kidneys of the weaning rats and in HEK-293 cells. The antioxidant mechanism of SGF related to activation of the Kelch-like ECH-associated protein 1/nuclear-factor-E2-related factor 2/hemeoxygenase-1(Keap1/Nrf2/HO-1) pathway. SGF could inhibit secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α induced by Pb in vivo and in vitro . The anti-inflammatory mechanism of SGF related to inhibition of ROS and pro-inflammatory cytokines triggered the nuclear factor-kappa B (NF-κB) pathway through blockade of inhibitors of I-κB degradation, phosphorylation of NF-κB p65, and nuclear translocation of p65. Our findings indicate that SGF could be a natural antioxidant and anti-inflammatory agent for treating lead-induced nephrotoxicity.

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Ginger extract protects rat’s kidneys against oxidative damage after chronic ethanol administration

Cited by 24 publications

References 39 publications

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

Long‐term ethanol consumption promotes changes in β‐defensin isoform gene expression and induces structural changes and oxidative DNA damage to the epididymis of rats

Protective Effects of Smilax glabra Roxb. Against Lead-Induced Renal Oxidative Stress, Inflammation and Apoptosis in Weaning Rats and HEK-293 Cells

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