Ginger Extract Inhibits Human Telomerase Reverse Transcriptase and c-Myc Expression in A549 Lung Cancer Cells

Tuntiwechapikul, Wirote; Taka, Thanachai; Songsomboon, Chonnipa; Kaewtunjai, Navakoon; Imsumran, Arisa; Makonkawkeyoon, Luksana; Pompimon, Wilart; Lee, T. Randall

doi:10.1089/jmf.2010.1191

Cited by 35 publications

(24 citation statements)

References 36 publications

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“…Our strongest indication of a treatment effect on proliferation was the estimated effect on hTERT expression. A decrease in hTERT expression is consistent with previous reports, which found that ginger inhibited hTERT and c-Myc expression in human non-small lung cancer cells (24). While the estimated treatment effect on our other marker of proliferation MIB-1 was not as strong, the estimated effect was more pronounced in the upper sections of the colorectal crypts, suggesting that ginger may decrease proliferation in the parts of the colorectal crypts most exposed to bowel lumen carcinogens.…”

Section: Discussionsupporting

confidence: 93%

Effects of Ginger Supplementation on Cell-Cycle Biomarkers in the Normal-Appearing Colonic Mucosa of Patients at Increased Risk for Colorectal Cancer: Results from a Pilot, Randomized, and Controlled Trial

Citronberg

Bostick

Ahearn

et al. 2013

Cancer Prevention Research

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To estimate the effects of ginger on apoptosis, proliferation, and differentiation in the normal-appearing colonic mucosa, we randomized 20 people at increased risk for colorectal cancer to 2.0 g of ginger or placebo daily for 28 days in a pilot trial. Overall expression and distributions of Bax, Bcl-2, p21, hTERT and MIB-1 (Ki-67) in colorectal crypts in rectal mucosa biopsies were measured using automated immunohistochemistry and quantitative image analysis. Relative to placebo, Bax expression in the ginger group decreased 15.6% (p = 0.78) in the whole crypts, 6.6% (p = 0.95) in the upper 40% (differentiation zone) of crypts, and 21.7% (p = 0.67) in the lower 60% (proliferative zone) of crypts; however, there was a 19% increase (p = 0.14) in Bax expression in the upper 40% relative to the whole crypt. While p21 and Bcl-2 expression remained relatively unchanged, hTERT expression in the whole crypts decreased by 41.2% (p = 0.05); the estimated treatment effect on hTERT expression was larger in the upper 40% of crypts (−47.9%; p = 0.04). In the ginger group, MIB-1 expression decreased in the whole crypts, upper 40% of crypts, and lower 60% of crypts by 16.9% (p = 0.39), 46.8% (p = 0.39), and 15.3% (p = 0.41), respectively. These pilot study results suggest that ginger may reduce proliferation in the normal-appearing colorectal epithelium and increase apoptosis and differentiation relative to proliferation—especially in the differentiation zone of the crypts, and support a larger study to further investigate these results.

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Section: Discussionsupporting

confidence: 93%

Effects of Ginger Supplementation on Cell-Cycle Biomarkers in the Normal-Appearing Colonic Mucosa of Patients at Increased Risk for Colorectal Cancer: Results from a Pilot, Randomized, and Controlled Trial

Citronberg

Bostick

Ahearn

et al. 2013

Cancer Prevention Research

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“…The hTERT promoter contains binding sites for c-Myc, and there evidence that c-Myc may increase hTERT expression [73–75]. Recently, it has been demonstrated that the ethyl acetate fraction of ginger extract inhibited growth of A549 lung carcinoma cells through downregulation of hTERT and c-Myc expression [76]. The findings in this study directly recapitulate these earlier studies, since treatment of MCF-7 and MDA MB-231 cells with ginger extract resulted in a significant reduction in the protein level of Bcl-2, which coincided with a parallel reduction in the protein levels of c-Myc and hTERT.…”

Section: Discussionmentioning

confidence: 99%

Differential Control of Growth, Apoptotic Activity, and Gene Expression in Human Breast Cancer Cells by Extracts Derived from Medicinal HerbsZingiber officinale

Elkady

Abu-Zinadah

Baeshen

et al. 2012

Journal of Biomedicine and Biotechnology

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The present study aimed to examine the antiproliferative potentiality of an extract derived from the medicinal plant ginger (Zingiber officinale) on growth of breast cancer cells. Ginger treatment suppressed the proliferation and colony formation in breast cancer cell lines, MCF-7 and MDA-MB-231. Meanwhile, it did not significantly affect viability of nontumorigenic normal mammary epithelial cell line (MCF-10A). Treatment of MCF-7 and MDA-MB-231 with ginger resulted in sequences of events marked by apoptosis, accompanied by loss of cell viability, chromatin condensation, DNA fragmentation, activation of caspase 3, and cleavage of poly(ADP-ribose) polymerase. At the molecular level, the apoptotic cell death mediated by ginger could be attributed in part to upregulation of Bax and downregulation of Bcl-2 proteins. Ginger treatment downregulated expression of prosurvival genes, such as NF-κB, Bcl-X, Mcl-1, and Survivin, and cell cycle-regulating proteins, including cyclin D1 and cyclin-dependent kinase-4 (CDK-4). On the other hand, it increased expression of CDK inhibitor, p21. It also inhibited the expression of the two prominent molecular targets of cancer, c-Myc and the human telomerase reverse transcriptase (hTERT). These findings suggested that the ginger may be a promising candidate for the treatment of breast carcinomas.

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“…8 Many plant extracts have been reported to inhibit cell proliferation through the down regulation of c-myc expression. 9,10 In this study, the c-myc expression was suppressed by γ-sitosterol which was isolated from S. crispus. To observe the effectiveness of γ-sitosterol in suppressing oncogenes, mRNAs was extracted from the treated cells.…”

Section: Discussionmentioning

confidence: 84%

Cytotoxic effect of γ-sitosterol from Kejibeling (<em>Strobilanthes crispus</em>) and its mechanism of action towards <em>c-myc</em> gene expression and apoptotic pathway

et al. 2015

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Abstrak Metode: Penelitian in vitro ini menggunakan kultur sel kanker kolon manusia (Caco-2), sel kanker hepar manusia (HepG2), sel kanker payudara tergantung hormon (MCF-7) dan kultur sel normal hepar manusia (Chang Liver). Efek sitotoksik diukur melalui uji MTT melalui penentuan Abstract Background:This study aimed to analyze the cytotoxicity effect of γ-sitosterol isolated from "Kejibeling" (Strobilanthes crispus), a medicinal plant, on several cancer cell lines. The mechanisms of the effects were studied through the expression of cancer-caused gene, c-myc and apoptotic pathways.

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Ginger Extract Inhibits Human Telomerase Reverse Transcriptase and c-Myc Expression in A549 Lung Cancer Cells

Cited by 35 publications

References 36 publications

Effects of Ginger Supplementation on Cell-Cycle Biomarkers in the Normal-Appearing Colonic Mucosa of Patients at Increased Risk for Colorectal Cancer: Results from a Pilot, Randomized, and Controlled Trial

Effects of Ginger Supplementation on Cell-Cycle Biomarkers in the Normal-Appearing Colonic Mucosa of Patients at Increased Risk for Colorectal Cancer: Results from a Pilot, Randomized, and Controlled Trial

Differential Control of Growth, Apoptotic Activity, and Gene Expression in Human Breast Cancer Cells by Extracts Derived from Medicinal HerbsZingiber officinale

Cytotoxic effect of γ-sitosterol from Kejibeling (<em>Strobilanthes crispus</em>) and its mechanism of action towards <em>c-myc</em> gene expression and apoptotic pathway

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