Ginger and Zingerone Ameliorate Lipopolysaccharide-Induced Acute Systemic Inflammation in Mice, Assessed by Nuclear Factor-κB Bioluminescent Imaging

Abstract

Ginger is a commonly used spice in cooking. In this study, we comprehensively evaluated the anti-inflammatory activities of ginger and its component zingerone in lipopolysaccharide (LPS)-induced acute systemic inflammation in mice via nuclear factor-κB (NF-κB) bioluminescent imaging. Ginger and zingerone significantly suppressed LPS-induced NF-κB activities in cells in a dose-dependent manner, and the maximal inhibition (84.5% ± 3.5% and 96.2% ± 0.6%) was observed at 100 μg/mL ginger and zingerone, respectively. Moreover, dietary ginger and zingerone significantly reduced LPS-induced proinflammatory cytokine production in sera by 62.9% ± 18.2% and 81.3% ± 6.2%, respectively, and NF-κB bioluminescent signals in whole body by 26.9% ± 14.3% and 38.5% ± 6.2%, respectively. In addition, ginger and zingerone suppressed LPS-induced NF-κB-driven luminescent intensities in most organs, and the maximal inhibition by ginger and zingerone was observed in small intestine. Immunohistochemical staining further showed that ginger and zingerone decreased interleukin-1β (IL-1β)-, CD11b-, and p65-positive areas in jejunum. In conclusion, our findings suggested that ginger and zingerone were likely to be broad-spectrum anti-inflammatory agents in most organs that suppressed the activation of NF-κB, the production of IL-1β, and the infiltration of inflammatory cells in mice.

Chemical Profiles and Protective Effect of Hedyotis diffusa Willd in Lipopolysaccharide-Induced Renal Inflammation Mice

“…LPS as an important inflammatory factor, could induce endotoxemia, shock and multiple organ dysfunction syndromes. During LPS-induced inflammatory process, multiple endogenous inflammatory mediators were produced in response to LPS, and the immune responses and organic functions were modulated [ 19 , 20 , 21 ]. The inflammatory model induced by LPS was commonly used to investigate the effect of herbal medicines and its bioactive constituents on renal inflammation [ 21 , 22 , 23 , 24 ].…”

“…During LPS-induced inflammatory process, multiple endogenous inflammatory mediators were produced in response to LPS, and the immune responses and organic functions were modulated [ 19 , 20 , 21 ]. The inflammatory model induced by LPS was commonly used to investigate the effect of herbal medicines and its bioactive constituents on renal inflammation [ 21 , 22 , 23 , 24 ]. In various acute and chronic renal inflammations, renal tubular epithelial cells actively participate in the process of glomerular sclerosis and renal fibrosis via producing all kinds of inflammatory cytokines, chemokines and extracellular matrix [ 25 ].…”

A Zingerone Analog, Acetyl Zingerone, Bolsters Matrisome Synthesis, Inhibits Matrix Metallopeptidases, and Represses IL-17A Target Gene Expression

“…Conversely, genes downregulated by AZ but upregulated by IL-17A were associated with epidermis development and inflammatory responses (Figure 6j). NF-kB is activated downstream of IL-17A in KCs (Wang et al, 2018) and inhibition of this transcription factor may mediate the anti-inflammatory effects of Z (Ahmad et al, 2018;Hsiang et al, 2015;Kim et al, 2010). Neither Znor AZ-regulated genes were enriched for motifs resembling the canonical NF-kB recognition sequence (5 0 -GGRRNNYYCC-3 0 ) in their 5-kilobase upstream regions (Supplementary Figure S10cee).…”

Pathological Study on the Role of Thymoquinone in Experimentally Induced Acute Lung Injury in Rats

“…Prophylactic and treatments attampts were applied to ameliorate the ALI induced by LPS through suppression of proinflammatory cytokines after treatment by acanthotic acid [4], andrographolide sulphate [5], mangolol [2], ginger extract or zingerone [14] and TQ [6,[15][16][17]. TQ is a natural product with main constituents of the volatile oil from Nigella sativa seeds which exhibits anti-inflammatory and anticancer activities [18].…”