Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly

Lechniak, D.; Warzych, Ewelina; Pers‐Kamczyc, Emilia; Sosnowski, J.; Antosik, Paweł; Rubeš, Jiřı́

doi:10.1016/j.theriogenology.2007.06.012

Cited by 33 publications

(24 citation statements)

References 36 publications

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“…Only 2 chromosomes (chromosome 1 and 10) were examined by FISH in this study. In a more recent study, applying the same technique and set of probes, Lechniak et al [2007] observed a significant difference in the oocyte aneuploidy rate in 4-5-month-old prepubertal gilts and 3.5-year-old sows. In the above study, 8 times more aneuploid oocytes (10.8%) were detected in gilts than in sows (1.3%).…”

Section: Discussionmentioning

confidence: 86%

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

Horňák

Hulinska

Musilová

et al. 2009

Cytogenet Genome Res

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Although numerical chromosome errors are known to be prevalent in early human embryos and are likely to be a considerable factor influencing the mortality of early embryos and implantation failure, in domestic animals data about the frequency and nature of errors is limited. The objectives of this study were to investigate the whole chromosome set of in vivo obtained early pig embryos, applying methods of whole genome amplification and comparative genomic hybridization (CGH) and to contribute to the comprehensive understanding of the topic. The embryos were collected from gilts 72 h after insemination. Further, they were lysed and underwent whole genome amplification by multiple displacement amplification. In a subsequent CGH, amplified DNA was compared to control DNA using different fluorescent labeling and hybridization to male pig mitoses. 11 (14.3%) of the 77 pig embryos examined were observed to be aneuploid. We found chromosome errors comprising loss/gain of one or a few chromosomes (10.4%) but also extensive chromosome imbalances (3.9%). Chromosomes 8, 11, 12, 13, 17, and X were most frequently involved in aneuploidies, when compared to chromosomes 2, 9, and 18, which were rarely involved in chromosome errors.

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Section: Discussionmentioning

confidence: 86%

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

Horňák

Hulinska

Musilová

et al. 2009

Cytogenet Genome Res

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“…The technique was principally used to analyze the oocyte quality by determining the diploidy and aneuploidy rates. In pigs the results obtained show proportions of diploid oocytes ranging from 5.9 to 27.7% and of aneuploidy from 1.3 to 21.6% (King et al, 1986;Koenig and Stormshak, 1993;Vozdova et al, 2001;Sosnowski et al, 2003;Lechniak et al, 2007). In cattle, the rate of diploid oocytes was estimated between 2 and 12.4% and the rate of aneuploidy between 5.8 and 7.1% (Yadav et al, 1991;Lechniak et al, 1996Lechniak et al, , 2002Sosnowski et al, 1996;Lechniak and Switonski, 1998).…”

Section: Analysis Of the Meiotic Productsmentioning

confidence: 99%

Chromosomal abnormalities, meiotic behavior and fertility in domestic animals

Villagómez

Pinton²

2008

Cytogenet Genome Res

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Since the advent of the surface microspreading technique for synaptonemal complex analysis, increasing interest in describing the synapsis patterns of chromosome abnormalities associated with fertility of domestic animals has been noticed during the past three decades. In spite of the number of scientific reports describing the occurrence of structural chromosome abnormalities, their meiotic behavior and gametic products, little is known in domestic animal species about the functional effects of such chromosome aberrations in the germ cell line of carriers. However, some interesting facts gained from recent and previous studies on the meiotic behavior of chromosome abnormalities of domestic animals permit us to discuss, in the frame of recent knowledge emerging from mouse and human investigations, the possible mechanism implicated in the well known association between meiotic disruption and chromosome pairing failure. New cytogenetic techniques, based on molecular and immunofluorescent analyses, are allowing a better description of meiotic processes, including gamete production. The present communication reviews the knowledge of the meiotic consequences of chromosome abnormalities in domestic animals.

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“…7. Chromosomal analysis of porcine oocytes (Giemsa staining); (right) haploid chromosome set (n=19), (left) diploid chromosome set (2n=38) (Lechniak et al, 2007) www.intechopen.com…”

Section: Karyotyping From Spreadsmentioning

confidence: 99%

“…Fluorescence in situ hybridization (FISH, Figure 8) is a well-established technique for chromosome analysis and aneuploidy screening (Foster et al, 2010;Lechniak et al, 2007). In contrast to previously described methods, FISH data can be obtained from interphase nuclei or at least in cases where not all chromosomes are separated during spreading.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

“…Unfortunately, the diagnostics of targeted aneuploidies in pig with fluorescence in-situ hybridization is limited to chromosome-specific DNA probes. Current protocols have used probes for up to 13 chromosomes in human samples but in pigs, they are suitable for usually two probes (specific for centromeric region of Chromosomes 1 and 10) (Lechniak et al, 2007). Results are thus extrapolated for all chromosomes solely based on these 2 probes.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

See 1 more Smart Citation

Comparing Pig and Amphibian Oocytes: Methodologies for Aneuploidy Detection and Complementary Lessons for MAPK Involvement in Meiotic Spindle Morphogenesis

Ješeta¹,

Bodart²

2012

Aneuploidy in Health and Disease

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Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly

Cited by 33 publications

References 36 publications

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

Investigation of Chromosome Aneuploidies in Early Porcine Embryos Using Comparative Genomic Hybridization

Chromosomal abnormalities, meiotic behavior and fertility in domestic animals

Comparing Pig and Amphibian Oocytes: Methodologies for Aneuploidy Detection and Complementary Lessons for MAPK Involvement in Meiotic Spindle Morphogenesis

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