Gilthead seabream (Sparus aurata L.) innate immune response after dietary administration of heat-inactivated potential probiotics

Díaz‐Rosales, Patricia; Salinas, Irene; Rodrı́guez, Alejandro; Cuesta, Alberto; Chabrillón, Mariana; Balebona, M.C.; Moriñigo, Miguel A.; Meseguer, José

doi:10.1016/j.fsi.2005.06.007

Cited by 148 publications

(84 citation statements)

References 27 publications

(28 reference statements)

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“…In another study, a significant increase in serum myeloperoxidase was observed as an early infection period response in P. sarana following an intraperitoneal challenge with A. hydrophila (Das et al, 2011). Similarly, serum PO levels increased in gilt-head seabream (Sparus aurata) exposed to Enteromyxum leei or heat inactivated probiotic bacteria (Diaz-Rosales et al, 2006). Thus, the higher peroxidase activity observed in challenged fish, not only documents the stimulatory effect of bacterial factors on neutrophilic granulocytes i.e.…”

Section: Discussionmentioning

confidence: 84%

Modulation of the innate immune responses in the striped snakehead murrel, Channa striata upon experimental infection with live and heat killed Aeromonas hydrophila

Priyadarshini¹,

Subramani²,

Michael³

2017

Open Vet J.

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It is well-known that the innate immune mechanisms in fish serve as the first line of defence against wide variety of pathogens. In most of the situations, innate responses get induced and enhanced after the pathogen invasion. It would be interesting to look into the inducibility of various innate immune mechanisms and the level of enhancement after infection with the pathogen. Hence, in the present investigation, modulation of the innate immune responses in the striped snakehead murrel, Channa striata on experimental challenge with either live virulent or heat killed Aeromonas hydrophila at a dose of 1x10 7 CFU (suspended in 0.2 mL PBS) were measured. Most of the non-specific (both humoral and cellular) immune responses tested were substantially induced or enhanced in both the experimental groups in comparison with the unchallenged control group. Significant increase in the lysozyme, total peroxidase, antiprotease and respiratory burst activities were observed after the pathogen challenge. Thus, most of the innate non-specific immune responses are inducible though they are constitutive of fish immune system exhibiting a basal level of activity even in the absence of pathogen challenge.

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Section: Discussionmentioning

confidence: 84%

Modulation of the innate immune responses in the striped snakehead murrel, Channa striata upon experimental infection with live and heat killed Aeromonas hydrophila

Priyadarshini¹,

Subramani²,

Michael³

2017

Open Vet J.

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“…The administration of probiotics as a control strategy has been shown to provide protection against many bacterial pathogens while reducing the dependency on antibiotics [1e4]. One of the fears surrounding the use of probiotics is that releasing live bacterial cells in large numbers into the vicinity of fish could interfere with the ecosystem [5]. Concerns have been voiced against the development of virulence traits through horizontal gene transfer, the pathogenicity to humans, and antagonism with other beneficial bacteria.…”

Section: Introductionmentioning

confidence: 99%

Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum

Sharifuzzaman¹,

Abbass²,

Tinsley³

et al. 2011

Fish & Shellfish Immunology

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The efficacy of cellular components of probiotics Kocuria SM1 and Rhodococcus SM2 to protect rainbow trout (Oncorhynchus mykiss, Walbaum) against vibriosis was assessed. Groups of fish (average weight = 10–15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e. 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ECPs), cell wall proteins (CWPs) and whole cell proteins (WCPs) of SM1 and SM2, respectively, or with 0.1 ml of phosphate-buffered saline (PBS) to serve as the control. Seven days after administration, fish from each group were challenged i.p. with 0.1 ml of a suspension in PBS of 3 × 105 cells ml−1 per fish of Vibrio anguillarum. Use of CWPs and WCPs demonstrated significantly (P < 0.05) better protection against V. anguillarum insofar as mortalities were reduced to 11–17% [relative percent survival (RPS) = 80–87%], although ECPs fared less well (mortalities = 33–38%; RPS = 56–62%; P > 0.05), compared to 86% mortalities of the controls. The mode of action reflected activation of innate immune factors by CWPs and WCPs, demonstrating significantly (P < 0.05) increased expression of respiratory burst (optical density; OD550nm) from 0.039 to 0.043–0.045, peroxidase (OD550nm) from 0.26 to 0.37–0.55, and bacterial killing activities (i.e. percentage of surviving bacteria reduced from 79% to 56–57% for SM2). Moreover, an elevation of leucocyte number (from 1.93% to 1.98–2.93%; P > 0.05) and immunoglubolin level (from 27 mg ml−1 to 28.5–33 mg ml−1; P > 0.05) were observed with the experimental groups. These results indicate that cell components of the probiotics stimulate an immune response

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“…However, the highest value of ACH50 was observed in T1 treatment. Similarly, the variations of ACH50 in rainbow trout and gilthead seabream fed different levels of probiotics were not statistically significant (Díaz-Rosales et al 2006). On the other hand, the value of ACH50 in Nile tilapia and olive flounder considerably increasedby either immersion or dietary administration of probiotics (Aly et al 2008, Wang et al 2008, Harikrishnan et al 2010.…”

Section: Discussionmentioning

confidence: 96%

“…In contrast, serum peroxidase activity in rainbow trout fed with B. subtilis remained unchanged (NewajFyzul et al 2007). Additionally, peroxidase contents in the serum and head kidney leucocytes of gilthead seabream, Sparus aurata, did not considerably vary during four weeks of dietary administration of probiotics (Díaz-Rosales et al 2006). The plasma peroxidase activity is usually measured as indicator of leucocytes activity (Quade and Roth 1997).…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Administration of Bacillus Probiotics on the Non-Specific Immune Responses of Tinfoil Barb, Barbonymus Schwanenfeldii (Actinopterygii: Cypriniformes: Cyprinidae)

Eslamloo¹,

Akhavan²,

Henry³

2013

Acta Icth et Piscat

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Gilthead seabream (Sparus aurata L.) innate immune response after dietary administration of heat-inactivated potential probiotics

Cited by 148 publications

References 27 publications

Modulation of the innate immune responses in the striped snakehead murrel, <i>Channa striata</i> upon experimental infection with live and heat killed <i>Aeromonas hydrophila</i>

Modulation of the innate immune responses in the striped snakehead murrel, <i>Channa striata</i> upon experimental infection with live and heat killed <i>Aeromonas hydrophila</i>

Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum

Effects of Dietary Administration of <I>Bacillus</I> Probiotics on the Non-Specific Immune Responses of Tinfoil Barb, <I>Barbonymus Schwanenfeldii</I> (Actinopterygii: Cypriniformes: Cyprinidae)

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