Gills of juvenile fish piaractus mesopotamicus as histological biomarkers for experimental sub-lethal contamination with the Organophosphorus Azodrin®400

Rudnicki, Cácia Aparecida Mendes; Melo, Gisele Cristiane de; Donatti, Lucélia; Kawall, Helena G.; Fanta, Edith

doi:10.1590/s1516-89132009000600015

Cited by 17 publications

(6 citation statements)

References 29 publications

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“…In Cirrhinus mrigala exposed to "Nuvan" at higher concentrations, appearance of aneurysm at an interval of 2 d and onward could cause the disruption/reduction in gaseous exchange capability and consequently weakening of the fishes. This corroborates with the views of Rudnicki et al (2009) [49] who reported aneurysm in secondary lamellae of the gills of Piaractus mesopotamicus exposed to Azodrin 400, an organophosphate. Machado and Fanta (2003) [37], in the gills of Metynnis roosevelti exposed to methyl parathion, an organophosphate, reported the collapse of the pillar cells followed by a loss of shape of the erythrocytes that can be considered to indicate osmotic and ionic alterations.…”

Section: Discussionsupporting

confidence: 92%

Alterations in the Gill Filaments and Secondary Lamellae ofCirrhinus mrigalaExposed to “Nuvan,” an Organophosphorus Insecticide

Srivastava

Kumārī

Kumari

et al. 2014

Journal of Histology

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The alterations in the epithelium of the gill filaments and the secondary lamellae of the gills ofCirrhinus mrigala, on exposure to “Nuvan,” have been explored in the present investigation using light and scanning electron microscopy. The fishes were exposed to two sublethal concentrations, 5 mg/L and 15 mg/L, of “Nuvan.” The changes are more rapid and intensive at higher concentration than at lower concentration, suggesting that the changes are dose dependent. Increase in thickness of epithelium covering secondary lamellae, merger of epithelium of gill filaments and adjacent secondary lamellae, and aneurysm is considered to reduce efficiency of gills for gaseous exchange. A significant decline in the density and area of the mucous goblet cells in the epithelium of the gill filaments and the secondary lamellae ofC. mrigalaexposed to “Nuvan” could be correlated with excessive loss of the secretory contents of these cells, uncompensated by their production in sufficient quantities. The histopathological changes, in general, take longer time to recover in the fishes exposed to 15 mg/L than those exposed to 5 mg/L indicating that the changes in fishes exposed to higher concentration are more severe than those at lower concentration of the insecticide.

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Section: Discussionsupporting

confidence: 92%

Alterations in the Gill Filaments and Secondary Lamellae ofCirrhinus mrigalaExposed to “Nuvan,” an Organophosphorus Insecticide

Srivastava

Kumārī

Kumari

et al. 2014

Journal of Histology

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“…All the histological observations indicated that exposure to sublethal concentration of MCP caused degenerative effects such as dense fibrosis of gill filaments,[ 11 ] fusion and shortening of secondary lamellae,[ 31 32 ] fragmented columnar cells, and hypertrophy in mucous cells of foot tissue. These changes in normal tissue architecture lead to loss of normal physiological functions of animal.…”

Section: Discussionmentioning

confidence: 99%

Assessment of toxicity of monocrotophos in freshwater bivalve, Lamellidens marginalis, using different markers

Mundhe¹,

Pandit²

2014

Toxicol Int

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The present study was undertaken to evaluate the toxic effects of monocrotophos, a widely used organophosphorus pesticide, on Lamellidens marginalis with a wide battery of biomarkers consisting of AchE inhibition, lipid peroxidation, the levels of antioxidant enzymes, and histopathological changes. Animals were exposed to monocrotophos (52.36 mg/l) for four days. Malondialdehyde (MDA) values were measured as index of oxidation while Superoxide dismutase (SOD), Catalase (CAT), Glutathione s-Transferase (GST), and Glutathione-Reductase (GR) were measured as index of an antioxidant status. After exposure, a significant reduction of the capability to neutralize radicals was observed. Histopathological changes, such as fibrosis in gill filaments and hypertrophy in mucous cells of foot tissue, were observed after treatment. In a second series of experiment, exposed animals were thereafter transferred to clean water and kept in it up to 28 days to assess the recovery pattern. Significant recovery is observed in AchE and antioxidant enzymes. Oxidative damage observed after acute exposure indicate that mussels faced an oxidative challenge but were able to counteract, as values of anti-oxidants returned near to control values after 28 days. Altered activities in anti-oxidant enzymes due to stress recovered well after 28 days in gill and muscles as compared to foot and mantle. Overall results suggested that oxidative markers are highly sensitive and could be profitably applied to freshwater mussels for environmental quality assessment in freshwater.

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“…The altered levels of antioxidant enzymes probably demonstrate a pollutant-induced toxic response in molluscs. [11] All the histological observations indicated that exposure to sublethal concentration of MCP caused degenerative effects such as dense fibrosis of gill filaments, [11] fusion and shortening of secondary lamellae, [31,32] fragmented columnar cells, and hypertrophy in mucous cells of foot tissue. These changes in normal tissue architecture lead to loss of normal physiological functions of animal.…”

Section: Mcp = Monocrotophos Lc = Lethal Concentrationmentioning

confidence: 99%

Toxicity of Monocrotophos in Freshwater Bivalve, Lamellidens marginalis, Using Different Markers

Tank¹

2022

IJRASET

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The present study was undertaken to evaluate the toxic effects of monocrotophos, a widely used organophosphorus pesticide, on Lamellidens marginalis with a wide battery of biomarkers consisting of AchE inhibition, lipid peroxidation, the levels of antioxidant enzymes, and histopathological changes. Animals were exposed to monocrotophos (52.36 mg/l) for four days. Malondialdehyde (MDA) values were measured as index of oxidation while Superoxide dismutase (SOD), Catalase (CAT), Glutathione s-Transferase (GST), and Glutathione-Reductase (GR) were measured as index of an antioxidant status. After exposure, a significant reduction of the capability to neutralize radicals was observed. Histopathological changes, such as fibrosis in gill filaments and hypertrophy in mucous cells of foot tissue, were observed after treatment. In a second series of experiment, exposed animals were there after transferred to clean water and kept in it up to 28 days to assess the recovery pattern. Significant recovery is observed in AchE and antioxidant enzymes. Oxidative damage observed after acute exposure indicate that mussels faced an oxidative challenge but were able to counteract, as values of anti-oxidants returned near to control values after 28 days.

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Gills of juvenile fish piaractus mesopotamicus as histological biomarkers for experimental sub-lethal contamination with the Organophosphorus Azodrin®400

Abstract: Piaractus mesopotamicus is a freshwater native fish from rivers of the Paraná-Paraguay Basin and of the

Cited by 17 publications

References 29 publications

Alterations in the Gill Filaments and Secondary Lamellae ofCirrhinus mrigalaExposed to “Nuvan,” an Organophosphorus Insecticide

Alterations in the Gill Filaments and Secondary Lamellae ofCirrhinus mrigalaExposed to “Nuvan,” an Organophosphorus Insecticide

Assessment of toxicity of monocrotophos in freshwater bivalve, Lamellidens marginalis, using different markers

Toxicity of Monocrotophos in Freshwater Bivalve, Lamellidens marginalis, Using Different Markers

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