Gill physiological and transcriptomic response of the threatened freshwater mussel Solenaia oleivora to salinity shift

Zhang, Ting; Yao, Jingting; Xu, Dongpo; Ma, Xueyan; Wu, Jin; Lv, Guohua; Gu, Ruobo; Huang, Wen; Zhou, Yanfeng

doi:10.1016/j.cbd.2021.100913

Cited by 10 publications

(7 citation statements)

References 44 publications

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Section: Discussionmentioning

confidence: 99%

“…Like most invertebrates, the immune system of mollusks consists mainly of non-specific immunity that is sensitive to various environmental stresses, such as salinity shift and air exposure ( Li et al, 2017 ; Zhang et al, 2021 ). ACP and AKP, two important hydrolases in lysosomes, are important in fighting disease and non-specific immune responses ( Zhang et al, 2021 ). It has been reported that the activities of the two enzymes can be affected by air exposure in shellfish, such as Pinctada fucata , Chlamys farreri , and Argopecten irradians ( Chen et al, 2007 ; Chi et al, 2017 ; Wei et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…These results demonstrated that short-term air exposure enhanced the antioxidant capacity of C. fluminea, but long-term air exposure caused lipid peroxidation damage in the hepatopancreas. Like most invertebrates, the immune system of mollusks consists mainly of non-specific immunity that is sensitive to various environmental stresses, such as salinity shift and air exposure (Li et al, 2017;Zhang et al, 2021). ACP and AKP, two important hydrolases in lysosomes, are important in fighting disease and non-specific immune responses (Zhang et al, 2021).…”

Section: Antioxidant Defense Transcript33245mentioning

confidence: 99%

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Histological, physiological, and transcriptomic responses of hepatopancreas to air exposure in asian freshwater clam Corbicula fluminea

Zhang

et al. 2022

Front. Physiol.

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Corbicula fluminea (C. fluminea) is an important freshwater economy shellfish in China, but it often suffers from air exposure during transportation. In this study, we investigated the histological, physiological (mainly including respiratory metabolism, antioxidant capacity, and immune function), and transcriptomic responses of hepatopancreas in C. fluminea to different times of air exposure. At histological level, air exposure caused vacuolation of digestive cells (24–96 h) and enlargement of digestive tubule lumen (6–96 h) in hepatopancreas. At physiological level, the activities of enzymes related to glycolysis (hexokinase and pyruvate kinase) and anaerobic respiration (lactate dehydrogenase) were increased first (6–24 h) of air exposure, then came back to normal level or even decreased. The activity of aerobic respiration-related enzyme (succinic dehydrogenase) began to reduce from 24 h of air exposure. The activities of antioxidant enzymes (superoxide dismutase and catalase) were enhanced during 6–48 h of air exposure and then returned to control level or even inhibited. The content of malondialdehyde (MDA) increased from 96 h of air exposure. The activities of immune-related enzymes (acid phosphatase and alkaline phosphatase) increased during 6–48 h, then returned to normal or began to decline. At transcriptome level, 44 differentially expressed genes (DEGs) in the hepatopancreas were identified after 96-h air exposure. Among these DEGs, 8 were associated with glycolysis, TCA cycle, immune, and antioxidant, and were downregulated after 96-h air exposure. Taken together, these findings illuminated the response of C. fluminea to air exposure at histological, physiological, and transcriptomic levels, which will be beneficial to the aquaculture and transportation of C. fluminea.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Antioxidant Defense Transcript33245mentioning

confidence: 99%

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Histological, physiological, and transcriptomic responses of hepatopancreas to air exposure in asian freshwater clam Corbicula fluminea

Zhang

et al. 2022

Front. Physiol.

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“…The elongation factor 1 alpha (EF1α) gene was utilized as a reference gene since our preliminary experiment showed that its expression did not respond to air exposure. The detailed experimental procedures were as previously described [ 65 ].…”

Section: Methodsmentioning

confidence: 99%

PacBio Full-Length and Illumina Transcriptomes of the Gill Reveal the Molecular Response of Corbicula fluminea under Aerial Exposure

Zhang

Huang

et al. 2022

IJMS

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Air exposure is a common stress for Corbicula fluminea, an economically important freshwater shellfish consumed in China, during aquaculture and transportation. However, little is known about its molecular responses to air exposure. Therefore, this study used a combination of PacBio full-length and Illumina transcriptomes to investigate its molecular responses to air exposure. A total of 36,772 transcripts were obtained using PacBio sequencing. Structural analysis identified 32,069 coding sequences, 1906 transcription factors, 8873 simple sequence repeats, and 17,815 long non-coding RNAs. Subcellular localization analysis showed that most transcripts were located in the cytoplasm and nucleus. After 96-h of air exposure, 210 differentially expressed genes (DEGs) in the gill were obtained via Illumina sequencing. Among these DEGs, most of the genes related to glycolysis, tricarboxylic acid cycle, lipid metabolism, and amino acid metabolism were upregulated. Additionally, many DEGs associated with immunity, cytoskeleton reorganization, autophagy, and ferroptosis were identified. These findings indicated that metabolic strategy change, immune response, cytoskeleton reconstruction, autophagy, and ferroptosis might be the important mechanisms that C. fluminea use to cope with air exposure. This study will enrich the gene resources of C. fluminea and provide valuable data for studying the molecular mechanisms coping with air exposure in C. fluminea and other freshwater mollusks.

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“…Gills are commonly considered a part of the respiratory system of aquatic animals ( Collinder et al, 2009 ; Carvalho, 2011 ; Ray and Ringø, 2014 ). In addition to respiration, fish gills are critical for excretion of nitrous waste, pH regulation, hormone production, and osmoregulation ( Foyle et al, 2020 ; Zhang et al, 2021 ). In most bivalves, gills have undergone a secondary adaptation to mainly serve as feeding structures while their function in osmoregulation and ion transport have been less studied ( Riisgård et al, 2011 , 2015 ; Moreira et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms

Romersi

Nicklisch

2022

Front. Physiol.

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An organism’s diet is a major route of exposure to both beneficial nutrients and toxic environmental chemicals and natural products. The uptake of dietary xenobiotics in the intestine is prevented by transporters of the Solute Carrier (SLC) and ATP Binding Cassette (ABC) family. Several environmental chemicals and natural toxins have been identified to induce expression of these defense transporters in fish and aquatic invertebrates, indicating that they are substrates and can be eliminated. However, certain environmental chemicals, termed Transporter-Interfering Chemicals or TICs, have recently been shown to bind to and inhibit fish and mammalian P-glycoprotein (ABCB1), thereby sensitizing cells to toxic chemical accumulation. If and to what extent other xenobiotic defense or nutrient uptake transporters can also be inhibited by dietary TICs is still unknown. To date, most chemical-transporter interaction studies in aquatic organisms have focused on ABC-type transporters, while molecular interactions of xenobiotics with SLC-type transporters are poorly understood. In this perspective, we summarize current advances in the identification, localization, and functional analysis of protective MXR transporters and nutrient uptake systems in the digestive system of fish and aquatic invertebrates. We collate the existing literature data on chemically induced transporter gene expression and summarize the molecular interactions of xenobiotics with these transport systems. Our review emphasizes the need for standardized assays in a broader panel of commercially important fish and seafood species to better evaluate the effects of TIC and other xenobiotic interactions with physiological substrates and MXR transporters across the aquatic ecosystem and predict possible transfer to humans through consumption.

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Gill physiological and transcriptomic response of the threatened freshwater mussel Solenaia oleivora to salinity shift

Cited by 10 publications

References 44 publications

Histological, physiological, and transcriptomic responses of hepatopancreas to air exposure in asian freshwater clam Corbicula fluminea

Histological, physiological, and transcriptomic responses of hepatopancreas to air exposure in asian freshwater clam Corbicula fluminea

PacBio Full-Length and Illumina Transcriptomes of the Gill Reveal the Molecular Response of Corbicula fluminea under Aerial Exposure

Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms

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