Gill (Na+,K+)-ATPase in diadromous, freshwater palaemonid shrimps: Species-specific kinetic characteristics and α-subunit expression

Santos, Lidia Caires; Belli, Nara Marcato; Augusto, Alessandra; Masui, Douglas Chodi; Leone, Francisco de Assis; McNamara, John Campbell; Furriel, Rosa dos Prazeres Melo

doi:10.1016/j.cbpa.2007.04.008

Cited by 64 publications

(101 citation statements)

References 59 publications

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“…During immersion, pH compensation is accomplished by ion exchange across the gill epithelia with 93% of e being taken up from the surrounding seawater, and only 7% coming from internal stores (Cameron, 1985). This involves branchial pumping mechanisms, such as Na + /K + -ATPase, that maintain haemolymph osmotic and acid-base homeostasis when exposed to disturbances via variations in abiotic drivers, such as salinity, pH, pCO 2 , pO 2 and temperature (Wood and Cameron, 1985;Whiteley, 1999;Whiteley et al, 2001;Freire et al, 2003;Lucu and Towle, 2003;Masui et al, 2005;Mendonça et al, 2007;Santos et al, 2007;Masui et al, 2009). These are characteristic of environments typified by rapidly fluctuating physico-chemical factors, such as the intertidal (Newell, 1979), and may be more pronounced in the future (IPCC, 2013).…”

Section: Introductionmentioning

confidence: 99%

Living in warmer more acidic oceans retards physiological recovery from tidal emersion in the velvet swimming crabNecora puber(L.)

Rastrick

Calosi

Calder-Potts

et al. 2014

Journal of Experimental Biology

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The distribution patterns of many species in the intertidal zone are partly determined by their ability to survive and recover from tidal emersion. During emersion, most crustaceans experience gill collapse, impairing gas exchange. Such collapse generates a state of hypoxemia and a hypercapnia-induced respiratory acidosis, leading to hyperlactaemia and metabolic acidosis. However, how such physiological responses to emersion are modified by prior exposure to elevated CO 2 and temperature combinations, indicative of future climate change scenarios, is not known. We therefore investigated key physiological responses of velvet swimming crabs, Necora puber, kept for 14 days at one of four pCO 2 /temperature treatments (400 μatm/10°C, 1000 μatm/10°C, 400 μatm/15°C or 1000 μatm/15°C) to experimental emersion and recovery. Preexposure to elevated pCO 2 and temperature increased pre-emersion bicarbonate ion concentrations , increasing resistance to short periods of emersion (90 min). However, there was still a significant acidosis following 180 min emersion in all treatments. The recovery of extracellular acid-base via the removal of extracellular pCO 2 and lactate after emersion was significantly retarded by exposure to both elevated temperature and pCO 2 . If elevated environmental pCO 2 and temperature lead to slower recovery after emersion, then some predominantly subtidal species that also inhabit the low to mid shore, such as N. puber, may have a reduced physiological capacity to retain their presence in the low intertidal zone, ultimately affecting their bathymetric range of distribution, as well as the structure and diversity of intertidal assemblages.

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Section: Introductionmentioning

confidence: 99%

Living in warmer more acidic oceans retards physiological recovery from tidal emersion in the velvet swimming crabNecora puber(L.)

Rastrick

Calosi

Calder-Potts

et al. 2014

Journal of Experimental Biology

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“…A ouabaína interage com a (Na + , K + )-ATPase a partir da superfície da membrana extracelular e seu sítio de ligação, inserido na membrana, é localizado em uma cavidade formada por segmentos da transmembrana M1, M2, M4, M5 e M6, muito próximo ao sitio de ligação do K + YATIME et al, 2011;LAURSEN et al, 2015 (SANTOS et al, 2007;LEONE et al, 2014), M.…”

Section: Figura 3 Esquema Da Estrutura Das Subunidades Da (Na + K +unclassified

“…O peso da isoforma foi muito parecida com a encontrada em diversas espécies, como Callinectes ornatos recém-capturado ou aclimatado a diferentes salinidades (GARÇON et. al., 2007;GARÇON et al, 2009); Callinectes danae (MASUI et al, 2002); Macrobrachium amazonicum (SANTOS et al, 2007) e Clibanarius simmetrycus recém-capturado ou aclimatado a elevada salinidade (GONÇALVES et. al., 2006;LUCENA et al, 2012).…”

Section: -Estudo Da Fração Microsomal De C Guanhumiunclassified

“…Os sítios de ligação de alta afinidade, muitas vezes observados para a hidrólise de ATP pela (Na + ,K + )-ATPase de crustáceos (MASUI et al, 2002;GONÇALVES et al, 2006;SANTOS et al, 2007;LUCENA et al, 2012), não foram revelados para a hidrólise de pnitrofenilfosfato, sugerindo que eles não estão disponíveis na forma E 2 da enzima de C.…”

Section: -Atividade Pnffase Da Fração Microsomal Do Tecido Branquial unclassified

“…Na ausência de íons magnésio a enzima não é capaz de hidrolisar o ATP, pois o complexo Mg-ATP é o verdadeiro substrado da (Na + ,K + )-ATPase (GLYNN, 1985;FURRIEL et al, 2000;KARLISH, 2003;GARÇON et al, 2007). Porém excesso de ATP ou Mg 2+ livre, inibem a atividade ATPase, como observado nesse estudo bem como para a enzima isolada de diversas fontes (ROBINSON, 1981;ROBINSON e PRATAP, 1991;TENTES e STRATAKIS, 1991;FURRIEL et al, 2000;MASUI et al, 2002;GONÇALVES et al, 2006;GARÇON et al, 2007;SANTOS et al, 2007). A afinidade da enzima de tecido branquial de C. guanhumi, recém-capturado e aclimatado à 22‰ S, pelos íons magnésio é muito similar aos valores relatados para a enzima de vertebrados (GLYNN, 1985;ROBINSON e PRATAP, 1991), membrana axonal de caranguejo (GACHE et al, 1976;ROSSI et al, 1978) e tecido branquial de M. olfersii (FURRIEL et al, 2000), C. danae (MASUI et al, 2002), C. vittatus (GONÇALVES et al, 2006) e X. kroyeri .…”

Section: -Efeito De Metais Divalentes Na Atividadeunclassified

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Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

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Osmoregulation and Excretion

Larsen

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The article discusses advances in osmoregulation and excretion with emphasis on how multicellular animals in different osmotic environments regulate their milieu intérieur. Mechanisms of energy transformations in animal osmoregulation are dealt with in biophysical terms with respect to water and ion exchange across biological membranes and coupling of ion and water fluxes across epithelia. The discussion of functions is based on a comparative approach analyzing mechanisms that have evolved in different taxonomic groups at biochemical, cellular and tissue levels and their integration in maintaining whole body water and ion homeostasis. The focus is on recent studies of adaptations and newly discovered mechanisms of acclimatization during transitions of animals between different osmotic environments. Special attention is paid to hypotheses about the diversity of cellular organization of osmoregulatory and excretory organs such as glomerular kidneys, antennal glands, Malpighian tubules and insect gut, gills, integument and intestine, with accounts on experimental approaches and methods applied in the studies. It is demonstrated how knowledge in these areas of comparative physiology has expanded considerably during the last two decades, bridging seminal classical works with studies based on new approaches at all levels of anatomical and functional organization. A number of as yet partially unanswered questions are emphasized, some of which are about how water and solute exchange mechanisms at lower levels are integrated for regulating whole body extracellular water volume and ion homeostasis of animals in their natural habitats. © 2014 American Physiological Society.

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Gill (Na+,K+)-ATPase in diadromous, freshwater palaemonid shrimps: Species-specific kinetic characteristics and α-subunit expression

Cited by 64 publications

References 59 publications

Living in warmer more acidic oceans retards physiological recovery from tidal emersion in the velvet swimming crabNecora puber(L.)

Living in warmer more acidic oceans retards physiological recovery from tidal emersion in the velvet swimming crabNecora puber(L.)

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Osmoregulation and Excretion

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