Gilbertaria, a first crustose genus in the Sphaerophoraceae (Lecanoromycetes, Ascomycota) for Catillaria contristans, Toninia squalescens and related species

Abstract

Lecideoid lichen-forming fungi are a large, heterogeneous group that includes many species described during the nineteenth century that are of unclear taxonomic status. We revise such a group, the species of which have previously been treated under the much-misunderstood names Catillaria contristans or Toninia squalescens, and use a seven-locus phylogeny to determine its phylogenetic position. We found strong support for a previously unrecognized monophyletic lineage within the Sphaerophoraceae, comprising five phylogenetic species, and describe the new genus Gilbertaria to accommodate them. The new genus is characterized by a crustose growth form, 1-septate ascospores, thick ((1.5–)2–3(–4) μm wide) paraphyses and asci of the Biatora-type. We revise the nomenclature and give new delimitations and descriptions of the Northern Hemisphere species Gilbertaria contristans comb. nov., G. holomeloides comb. nov., G. squalescens comb. nov. and describe the new species G. astrapeana from the Falkland Islands.

Nimisora (Lecanoraceae, Ascomycota), a new genus for a common lecideoid epiphytic species from the central Iberian Peninsula

“…Magn.) Printzen and the nrITS and mtSSU amplified following the methods described in Svensson & Fryday (2022). PCR products were purified using Exo SAP-IT (USB Europe GmbH), following the manufacturer's instructions.…”

Pyxine tiinae (Caliciaceae, Ascomycota), a new lichen species from high elevation in Peru

“…We extracted DNA and used primers and PCRprotocols following the procedures described in Svensson & Fryday (2022). For LSU, we used the primers LR3 and LR5 (Vilgalys & Hester, 1990) in combination with LRLecF (Schneider et al, 2015), using the same PCR thermal profile as Svensson & Fryday used for LSU.…”

“…For all these new primers, we used the VWR Red Taq Polymerase Master Mix (VWR International, Belgium) following the manufacturer's protocols. For mtSSU-PyxF and mtSSU-PyxR, we used the same PCR thermal profile as the one used for ITS by Svensson & Fryday (2022). We used MCM7-PyxF and MCM7-PyxR as nested primers after running a first PCR with the primers MCM7-709f and MCM7-1348r (Schmitt et al, 2009), in both cases with the same PCR thermal profile as was used for MCM7 by Svensson & Fryday (2022).…”