Gibberellins Are Not Required for Normal Stem Growth in <i>Arabidopsis thaliana</i> in the Absence of GAI and RGA

King, Kathryn E.; Moritz, Thomas; Harberd, Nicholas P.

doi:10.1093/genetics/159.2.767

Cited by 246 publications

(21 citation statements)

References 34 publications

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“…This indicates that BcRGA1, BcRGA2, BcRGL1, and BcRGL3 may inhibit stem growth and development, whereas GA 3 treatment, which degrades them, initiates stem elongation and development. These findings are consistent with the current understanding of DELLA proteins as GA-responsive repressors of plant growth [17]. RGL2 is a negative regulator of GA responses that acts specifically to control seed germination rather than stem elongation [19].…”

Section: Discussionsupporting

confidence: 89%

“…These DELLA proteins belong to the plant-specific GRAS regulatory protein gene family [6,11]. GAI and RGA are crucial for the regulation of plant stem elongation growth in response to GA [13][14][15] and have a functional overlap in inhibiting plant elongation [16,17]. RGL1 and RGL2 play important roles in controlling flower bud differentiation and flower development, respectively [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…GAI and RGA are crucial for the regulation of plant stem elongation growth in response to GA [13][14][15] and have a functional overlap in inhibiting plant elongation [16,17]. RGL1 and RGL2 play important roles in controlling flower bud differentiation and flower development, respectively [16][17][18][19]. RGL3 acts as a positive regulator in the defense response but performs a minor function in plant development processes [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Identification of DELLA Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage

Guan

Huang

Zhu

et al. 2021

IJMS

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Flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) is an important and extensively cultivated vegetable in south China, and its stalk development is mainly regulated by gibberellin (GA). DELLA proteins negatively regulate GA signal transduction and may play an important role in determining bolting and flowering. Nevertheless, no systematic study of the DELLA gene family has been undertaken in flowering Chinese cabbage. In the present study, we found that the two-true-leaf spraying of gibberellin A3 (GA3) did not promote bolting but did promote flowering, whereas the three-true-leaf spraying of GA3 promoted both bolting and flowering. In addition, we identified five DELLA genes in flowering Chinese cabbage. All five proteins contained DELLA, VHYNP, VHIID, and SAW conserved domains. Protein-protein interaction results showed that in the presence of GA3, all five DELLA proteins interacted with BcGID1b (GA-INSENSITIVE DWARF 1b) but not with BcGID1a (GA-INSENSITIVE DWARF 1a) or BcGID1c (GA-INSENSITIVE DWARF 1c). Their expression analysis showed that the DELLA genes exhibited tissue-specific expression, and their reversible expression profiles responded to exogenous GA3 depending on the treatment stage. We also found that the DELLA genes showed distinct expression patterns in the two varieties of flowering Chinese cabbage. BcRGL1 may play a major role in the early bud differentiation process of different varieties, affecting bolting and flowering. Taken together, these results provide a theoretical basis for further dissecting the DELLA regulatory mechanism in the bolting and flowering of flowering Chinese cabbage.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of DELLA Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage

Guan

Huang

Zhu

et al. 2021

IJMS

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“…The first group with two ‘ duli ’ pear DELLAs, Chr16.g31263.m1 and Chr13.g24360.m1, also contained apple MdRGL1a (DQ007885) and MdRGL1b (DQ007886), Arabidopsis AtGAI (AT1G14920) and AtRGA (AT2G01570), grape VvGAI (AF378125), barley SLN1 (AF460219), wheat Rht-D1a (AJ242531), rice OsSLR1 (AB030956), and maize D8 (AJ242530). Importantly, this group of DELLAs is well known for their regulation of plant height because their knockout mutants exhibit dwarfism, such as wheat rht-D1a1 and rice sly1 [ 18 , 22 , 34 , 35 , 36 , 37 ]. Therefore, we named Chr16.g31263.m1 and Chr13.g24360.m1 as PbGAI1a and PbGAI1b, respectively.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘duli’ Pear (Pyrus betulifolia Bunge)

Song

et al. 2022

IJMS

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Gibberellic acid (GA) is an important phytohormone that regulates every aspect of plant growth and development. While elements involved in GA signaling have been identified and, hence, their functions have been well studied in model plants, such as Arabidopsis and rice, very little is known in pear. We, therefore, analyzed the genes related to GA signaling from the recently sequenced genome of the wildtype ‘duli’ pear (Pyrus betulifolia Bunge), a widely used rootstock for grafting in pear cultivation in China due to its vigorous growth and resistance to abiotic and biotic stress. In total, 15 genes were identified, including five GA receptors PbGID1s (GA-INSENSTIVE DWARF 1), six GA negative regulators, PbDELLAs, and four GA positive regulators, PbSLYs. Exogenous application of GA could promote the expression of PbGID1s but inhibit that of PbDELLAs and PbSLYs in tissue culture ‘duli’ pear seedlings. The expression profiles of these genes in field-grown trees under normal growth conditions, as well as in tissue-cultured seedlings treated with auxin (IAA), GA, paclobutrazol (PAC), abscisic acid (ABA), and sodium chloride (NaCl), were also studied, providing further evidence of the involvement of these genes in GA signaling in ‘duli’ pear plants. The preliminary results obtained in this report lay a good foundation for future research into GA signaling pathways in pear. Importantly, the identification and preliminary functional verification of these genes could guide molecular breeding in order to obtain the highly desired dwarf pear rootstocks for high-density plantation to aid easy orchard management and high yielding of pear fruits.

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“…The nox1 mutant seeds (He et al, 2004) were provided by Yikun He. The gai-t6 rga-24 , gai-t6 rga-t2 rgl1-1 rgl2-1 , and pRGA :: GFP-RGA transgenic line (L er background) (Achard et al, 2006; King et al, 2001; Silverstone et al, 2001) were provided by Xiangdong Fu. The rga (SALK_089146) mutant was obtained from ABRC.…”

Section: Star*methodsmentioning

confidence: 99%

Nitric oxide negatively regulates gibberellin signaling to coordinate growth and salt tolerance in Arabidopsis

Chen

Sun

Song

et al. 2022

Preprint

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SummaryIn response to dynamically altered environments, plants must finely coordinate the balance between growth and stress responses for their survival. However, the underpinning regulatory mechanisms remain largely elusive. The phytohormone gibberellin promotes growth via a derepression mechanism by proteasomal degradation of the DELLA transcription repressors. Conversely, the stress-induced burst of nitric oxide (NO) enhances stress tolerance, largely relaying on NO-mediated S-nitrosylation, a redox-based posttranslational modification. Here, we show that S-nitrosylation of Cys-374 in the Arabidopsis RGA protein, a key member of DELLAs, inhibits its interaction with the F-box protein SLY1, thereby preventing its proteasomal degradation under salinity condition. The accumulation of RGA consequently retards growth but enhances salt tolerance. We propose that NO negatively regulates gibberellin signaling via S-nitrosylation of RGA to coordinate the balance of growth and stress responses when challenged by adverse environments.

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Gibberellins Are Not Required for Normal Stem Growth in Arabidopsis thaliana in the Absence of GAI and RGA

Cited by 246 publications

References 34 publications

Identification of DELLA Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage

Identification of DELLA Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage

Genome-Wide Analysis of Genes Involved in the GA Signal Transduction Pathway in ‘duli’ Pear (Pyrus betulifolia Bunge)

Nitric oxide negatively regulates gibberellin signaling to coordinate growth and salt tolerance in Arabidopsis

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