Gibberellin Mobilizes Distinct DELLA-Dependent Transcriptomes to Regulate Seed Germination and Floral Development in Arabidopsis

Cao, Dongni; Cheng, Hui; Wu, Wei; Soo, Hui Meng; Peng, Jinrong

doi:10.1104/pp.106.082289

Cited by 245 publications

(303 citation statements)

References 82 publications

(108 reference statements)

Supporting

Mentioning

286

Contrasting

Unclassified

Order By: Relevance

“…GA increases growth by causing degradation of DELLA growth repressor proteins, and also through DELLA-independent mechanisms (Peng et al, 1997;Fu et al, 2002;Cheng et al, 2004;Cao et al, 2006). Therefore, we investigated the involvement of DELLA proteins in Suc-induced hypocotyl elongation under light/ dark cycles.…”

Section: Phytohormone Signaling and Suc-induced Hypocotyl Elongation mentioning

confidence: 99%

“…5B). One possibility is that DELLA-independent GA signaling contributes to Suc-induced hypocotyl elongation, because DELLA proteins control approximately 40% to 60% of GA-regulated transcripts (Cao et al, 2006). An alternative possibility is that these were off-target or ectopic effects of paclobutrazol, because the paclobutrazolinduced attenuation of hypocotyl elongation was not rescued fully by GA supplementation (Fig.…”

Section: Involvement Of Phytohormone Signals In Suc-induced Hypocotylmentioning

confidence: 99%

See 1 more Smart Citation

The Energy-Signaling Hub SnRK1 Is Important for Sucrose-Induced Hypocotyl Elongation

et al. 2017

View full text Add to dashboard Cite

Emerging seedlings respond to environmental conditions such as light and temperature to optimize their establishment. Seedlings grow initially through elongation of the hypocotyl, which is regulated by signaling pathways that integrate environmental information to regulate seedling development. The hypocotyls of Arabidopsis (Arabidopsis thaliana) also elongate in response to sucrose. Here, we investigated the role of cellular sugar-sensing mechanisms in the elongation of hypocotyls in response to Suc. We focused upon the role of SnRK1, which is a sugar-signaling hub that regulates metabolism and transcription in response to cellular energy status. We also investigated the role of TPS1, which synthesizes the signaling sugar trehalose-6-P that is proposed to regulate SnRK1 activity. Under light/dark cycles, we found that Suc-induced hypocotyl elongation did not occur in tps1 mutants and overexpressors of KIN10 (AKIN10/SnRK1.1), a catalytic subunit of SnRK1. We demonstrate that the magnitude of Suc-induced hypocotyl elongation depends on the day length and light intensity. We identified roles for auxin and gibberellin signaling in Suc-induced hypocotyl elongation under short photoperiods. We found that Suc-induced hypocotyl elongation under light/dark cycles does not involve another proposed sugar sensor, HEXOKINASE1, or the circadian oscillator. Our study identifies novel roles for KIN10 and TPS1 in mediating a signal that underlies Suc-induced hypocotyl elongation in light/dark cycles.

show abstract

Section: Phytohormone Signaling and Suc-induced Hypocotyl Elongation mentioning

confidence: 99%

Section: Involvement Of Phytohormone Signals In Suc-induced Hypocotylmentioning

confidence: 99%

The Energy-Signaling Hub SnRK1 Is Important for Sucrose-Induced Hypocotyl Elongation

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Among the genes shown to be regulated by GA were two subsequently shown to encode the GID1 homologs GID1a and GID1b (data not shown). Furthermore, a recent transcriptomics study by Cao et al (2006) identified GID1a and GID1b as DELLA-upregulated genes. We examined this further by quantitative RT-PCR analysis and confirmed that indeed a feedback mechanism, similar to that operating in GA biosynthesis, also appears to modulate transcript levels of GID1a, GID1b, and GID1c ( Figures 1B and 1C).…”

Section: Reproductive Growth and Developmentmentioning

confidence: 99%

Genetic Characterization and Functional Analysis of the GID1 Gibberellin Receptors inArabidopsis

et al. 2006

View full text Add to dashboard Cite

We investigated the physiological function of three Arabidopsis thaliana homologs of the gibberellin (GA) receptor GIBBERELLIN-INSENSITIVE DWARF1 (GID1) by determining the developmental consequences of GID1 inactivation in insertion mutants. Although single mutants developed normally, gid1a gid1c and gid1a gid1b displayed reduced stem height and lower male fertility, respectively, indicating some functional specificity. The triple mutant displayed a dwarf phenotype more severe than that of the extreme GA-deficient mutant ga1-3. Flower formation occurred in long days but was delayed, with severe defects in floral organ development. The triple mutant did not respond to applied GA. All three GID1 homologs were expressed in most tissues throughout development but differed in expression level. GA treatment reduced transcript abundance for all three GID1 genes, suggesting feedback regulation. The DELLA protein REPRESSOR OF ga1-3 (RGA) accumulated in the triple mutant, whose phenotype could be partially rescued by loss of RGA function. Yeast two-hybrid and in vitro pull-down assays confirmed that GA enhances the interaction between GID1 and DELLA proteins. In addition, the N-terminal sequence containing the DELLA domain is necessary for GID1 binding. Furthermore, yeast three-hybrid assays showed that the GA-GID1 complex promotes the interaction between RGA and the F-box protein SLY1, a component of the SCF SLY1 E3 ubiquitin ligase that targets the DELLA protein for degradation.

show abstract

“…To date, several microarray analyses have been carried out to identify genes affected by DELLA proteins or GA in Arabidopsis at different developmental stages, including seed germination and seedling and floral development (Ogawa et al, 2003;Cao et al, 2006;Nemhauser et al, 2006;Zentella et al, 2007). Among these studies, Cao et al (2006) compared transcriptomes in developing flowers of ga1-3, ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1, and wild-type plants.…”

mentioning

confidence: 99%

“…Among these studies, Cao et al (2006) compared transcriptomes in developing flowers of ga1-3, ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1, and wild-type plants. That study revealed that GA could regulate downstream genes during flower development in either a DELLA-dependent or a DELLA-independent manner.…”

mentioning

confidence: 99%

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Hou

Shen

et al. 2008

Plant Physiology

View full text Add to dashboard Cite

Gibberellin (GA) plays important roles in regulating many aspects of plant development. GA derepresses its signaling pathway by promoting the degradation of DELLA proteins, a family of nuclear growth repressors. Although the floral organ identity is established in flowers of the GA-deficient mutant ga1-3, the growth of all floral organs is severely retarded. In particular, abortive anther development in ga1-3 results in male sterility. Genetic analysis has revealed that various combinations of null mutants of DELLA proteins could gradually rescue floral organ defects in ga1-3 and that RGA is the most important DELLA protein involved in floral organ development. To elucidate the early molecular events controlled by RGA during flower development, we performed whole-genome microarray analysis to identify genes in response to the steroid-inducible activation of RGA in ga1-3 rgl2 rga 35S:RGA-GR. Although DELLA proteins were suggested as transcriptional repressors, similar numbers of genes were down-regulated or up-regulated by RGA during floral organ development. More than one-third of RGA down-regulated genes were specifically or predominantly expressed in stamens. A significant number of RGA-regulated genes are involved in phytohormone signaling or stress response. Further expression analysis through activation of RGA by steroid induction combined with cycloheximide identified eight genes as immediate targets of RGA. In situ hybridization and transgenic studies further showed that the expression pattern and function of several selected genes were consistent with the predictions from microarray analysis. These results suggest that DELLA regulation of floral organ development is modulated by multiple phytohormones and stress signaling pathways.

show abstract

Gibberellin Mobilizes Distinct DELLA-Dependent Transcriptomes to Regulate Seed Germination and Floral Development in Arabidopsis

Cited by 245 publications

References 82 publications

The Energy-Signaling Hub SnRK1 Is Important for Sucrose-Induced Hypocotyl Elongation

The Energy-Signaling Hub SnRK1 Is Important for Sucrose-Induced Hypocotyl Elongation

Genetic Characterization and Functional Analysis of the GID1 Gibberellin Receptors inArabidopsis

Global Identification of DELLA Target Genes during Arabidopsis Flower Development

Contact Info

Product

Resources

About