Gibberellin disturbs the balance of endogenesis hormones and inhibits adventitious root development of Pseudostellaria heterophylla through regulating gene expression related to hormone synthesis

Zhang, Jinqiang; Zhou, Tao; Zhang, Chen; Zheng, Wei; Jun, Li; Jiang, Wei-Ke; Xiao, Chao; Wei, Dequn; Yang, Chun-Lin; Xu, Rui; Gong, Anhui; Bi, Yan

doi:10.1016/j.sjbs.2020.09.022

Cited by 17 publications

(16 citation statements)

References 27 publications

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“…Previous studies have shown that ABA, CTK, and JA increased gradually with the development of tuberous roots in P . heterophylla [ 43 ], and which involved in the regulation of cell proliferation, cell elongation, cell expansion [ 44 ], formation of microtubule microfilaments [ 45 ] and accumulation of assimilates in tuberous roots [ 46 ]. In this study, several key genes in hormone signal transduction were targeted by miRNAs from P .…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of miRNAome and target genes in Pseudostellaria heterophylla

Wang

Zhou

et al. 2022

PLoS ONE

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miRNAs play a crucial role in the development and growth of plants by inhibiting the function of targeted genes at the post-transcription level. However, no miRNAs in Pseudostellaria heterophylla have been reported and their function in the morphogenesis of organs is still unclear. In this study, a total of 159 conserved miRNAs (belonging to 64 families) and 303 level miRNAs were identified from P. heterophylla. Some of them showed specifically up or down-regulated expression in different tissues and numbers of unigenes involved in Plant-pathogen interaction and MAPK signaling pathway-plant were targeted. The significant negative correlation of expression profiles between 30 miRNAs and their target genes (37 unigenes) was observed, respectively. Further, a large number of genes involved with signal transduction of auxin, zeatin, abscisic acid and, jasmonic acid were targeted. Predicated targets of two miRNAs were validated by 5′RLM-RACE, respectively. A large number of mRNAs from four pathogens were targeted by miRNAs from P. heterophylla and some of them were targeted by miR414. In summary, we reported a population of miRNAs from four different vegetative tissues of P. heterophylla by high throughput sequencing, which was analyzed by combining with the constructed transcriptome. These results may help to explain the function of miRNAs in the morphogenesis of organs and defense of pathogens, and may provide theoretical basis for breeding and genetic improvement of P. heterophylla.

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of miRNAome and target genes in Pseudostellaria heterophylla

Wang

Zhou

et al. 2022

PLoS ONE

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“…However, it still needs further validation whether exogenous gibberellin can increase the content of GA 3 in stems by entering different parts of plants or stimulate the synthesis of more gibberellin in the site of synthesis. In addition, Zhang et al [ 26 ] found that the application of GA 3 to Pseudostellaria heterophylla promoted elongation of the stems, while the application of paclobutrazol did not alter the promoting effect of GA 3 . In the present study, we applied uniconazole (S 3307 ) to the third internode, while GA 3 was applied to the second internode of soybean plants in the V2 stage and found that S 3307 reduced the stem GA 3 content and inhibited the promotion of internode elongation by GA 3 , which further verifies that the GA 3 level in the stem is an important factor affecting soybean internode elongation.…”

Section: Discussionmentioning

confidence: 99%

“…We speculated that the stem elongation and growth patterns of different plants were different, which led to the differences in cell shape changes. After GA 3 was applied, the xylem radius and the number of xylem cell layers of Pseudostellaria heterophylla plants increased significantly, but the width of the secondary phloem, the number of secondary phloem cell layers, and the width of the vascular cambium did not significantly change [ 26 ]. The application of GA 3 to different transgenic Arabidopsis plants significantly increased the proportion of xylem area relative to the total area [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…It was also shown that the application of GA3 to stems, leave roots could affect internode elongation. However, it still needs further vali whether exogenous gibberellin can increase the content of GA3 in stems by enteri ferent parts of plants or stimulate the synthesis of more gibberellin in the site of syn In addition, Zhang et al [26] found that the application of GA3 to Pseudostellaria phylla promoted elongation of the stems, while the application of paclobutrazol d alter the promoting effect of GA3. In the present study, we applied uniconazole (S the third internode, while GA3 was applied to the second internode of soybean pl The values are presented as the means ± standard errors (n = 8).…”

Section: Discussionmentioning

confidence: 99%

“…Sauter et al [ 22 ] reported that GA 3 treatment promotes stem elongation by altering the orientation of cellulose microfibrils and that GAs also promote xylem differentiation, regulate cambial cell division, and induce the formation of secondary xylem fibers [ 23 , 24 , 25 ]. GA 3 treatment of Pseudostellaria heterophylla significantly increased the xylem radius and the number of xylem cell layers in the plants, but it had no significant effect on the width of the secondary phloem, cell layers, or vascular cambium [ 26 ]. GA 3 treatment of Arabidopsis also resulted in a significantly increased proportion of xylem [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Study on the Regulatory Effects of GA3 on Soybean Internode Elongation

Shan

Jin

Wang

et al. 2021

Plants

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Excessive plant height is an important factor that can lead to lodging, which is closely related to soybean yield. Gibberellins are widely used as plant growth regulators in agricultural production. Gibberellic acid (GA3), one of the most effective active gibberellins, has been used to regulate plant height and increase yields. The mechanism through which GA3 regulates internode elongation has been extensively investigated. In 2019 and 2020, we applied GA3 to the stems, leaves, and roots of two soybean cultivars, Heinong 48 (a high-stalk cultivar) and Henong 60 (a dwarf cultivar), and GA3 was also applied to plants whose apical meristem was removed or to girded plants to compare the internode length and stem GA3 content of soybean plants under different treatments. These results suggested that the application of GA3 to the stems, leaves, and roots of soybean increased the internode length and GA3 content in the stems. Application of GA3 decreased the proportion of the pith in the soybean stems and primary xylem while increasing the proportion of secondary xylem. The apical meristem is an important site of GA3 synthesis in soybean stems and is involved in the regulation of stem elongation. GA3 was shown to be transported acropetally through the xylem and laterally between the xylem and phloem in soybean stems. We conclude that the GA3 level in stems is an important factor affecting internode elongation.

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Hydrogen Peroxide is Involved in Salicylic Acid-Induced Adventitious Rooting in Cucumber Under Cadmium Stress

et al. 2021

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Gibberellin disturbs the balance of endogenesis hormones and inhibits adventitious root development of Pseudostellaria heterophylla through regulating gene expression related to hormone synthesis

Cited by 17 publications

References 27 publications

Identification and characterization of miRNAome and target genes in Pseudostellaria heterophylla

Identification and characterization of miRNAome and target genes in Pseudostellaria heterophylla

Study on the Regulatory Effects of GA3 on Soybean Internode Elongation

Hydrogen Peroxide is Involved in Salicylic Acid-Induced Adventitious Rooting in Cucumber Under Cadmium Stress

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