Gibberellin Biosynthesis And Control

Graebe, Jan E.

doi:10.1146/annurev.arplant.38.1.419

Cited by 190 publications

(261 citation statements)

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“…1). Because the critical role of GA in the control of internode elongation is generally recognized (Graebe, 1987), the longer internode length of late-maturing cultivars suggests the higher level of endogenous GA in their shoots.…”

Section: Resultsmentioning

confidence: 99%

Effects of Jasmonates on in vitro Tuberization in Several Potato Cultivars that Differ Greatly in Maturity

Koda

Kikuta

2001

Plant Production Science

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Section: Resultsmentioning

confidence: 99%

Effects of Jasmonates on in vitro Tuberization in Several Potato Cultivars that Differ Greatly in Maturity

Koda

Kikuta

2001

Plant Production Science

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“…1. GA biosynthetic pathways to C20-tricarboxylic acids (GA25, GA13, GA43) and C19-GAs (GA9, GA4) as found in pumpkin endosperm (2,3) and reactions shown to be catalyzed by recombinant 7-oxidase (A) and 20-oxidase (B).…”

Section: Methodsmentioning

confidence: 99%

“…Their biosynthesis has been extensively studied in immature pumpkin seeds (2)(3)(4), which are a rich source of GA biosynthetic enzymes and their encoding mRNAs (5-7). The biosynthetic pathway proceeds from the common intermediate, GA 12 aldehyde, as illustrated in Fig.…”

mentioning

confidence: 99%

Cloning gibberellin dioxygenase genes from pumpkin endosperm by heterologous expression of enzyme activities in Escherichia coli

Lange

1997

Proc. Natl. Acad. Sci. U.S.A.

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Gibberellin (GA) plant hormones are biosynthesized via complex pathways, the final steps of which are catalyzed by 2-oxoglutarate-dependent dioxygenases. Here, the cloning of two such enzymes, the GA 7-oxidase and the GA 20-oxidase, is reported using a novel approach, namely, by screening for GA dioxygenase activities expressed as T7 gene 10 fusion proteins in recombinant Escherichia coli. In vitro translation products of mRNA from endosperm of immature pumpkin seeds contained three GA dioxygenase activities, including 7-oxidase, 20-oxidase, and 3␤-hydroxylase. A cDNA expression library was prepared from the endosperm mRNA in MOSElox. An aliquot of the amplified library was converted to plasmids in vivo and used for transformation of E. coli BL21(DE3), which thereafter expressed recombinant fusion proteins containing 7-oxidase, 20-oxidase, and 3␤-hydroxylase activities. By screening for specific GA dioxygenase expression, clones harboring 7-oxidase and 20-oxidase cDNA were isolated. The ORF of the 7-oxidase cDNA is 945 bp long, encoding for 314 amino acid residues with a calculated M r of 35,712 and pI of 5.7. Recombinant GA 7-oxidase oxidizes GA 12 -aldehyde to GA 12 and GA 14 -aldehyde to GA 14 . Evidence was obtained for further metabolism of GA 12 by the 7-oxidase to four products, two of which are monohydroxylated GA 12 . The ORF of the 20-oxidase is-apart from seven changes, resulting in four amino acid substitutions-identical to the 20-oxidase cDNA previously cloned from pumpkin cotyledon mRNA; both 20-oxidases have the same catalytic properties.

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“…에서 사용할 경우 시들음 증상을 감소시키고 (Smith et al, 1991), 생체내(in vivo)에서 사용할 경우 키가 큰 식물의 절 간을 짧게 하고, 잎의 크기 감소시키며, 잎을 진녹색으로 변 화시켜 지상부를 튼튼하게 만들 뿐만 아니라 뿌리를 두껍게 하여 지하부도 단단하게 만든다 (Graebe, 1987). 또한 수선화 (Chen and Ziv, 2001a, b), 마늘 (Kim et al, 2003), Colchicum autumnale (Ellington et al, 1997), 양파(Guen-Le Saos et al,…”

Section: 식물생장억제제는 조직배양묘의 순화 시 발생되는 식물 체의 고사 문제를 극복하기 위해 사용하는데 기내(In unclassified

Optimalization of Flurprimidol Concentration for Improvement of Acclimatization Rate and In Vitro Growth Inhibition in Bioreactor Culture of Strawberry Plantlets via Bioreactor Culture

Kim¹,

Lee²,

Kim³

et al. 2015

HST

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This study was conducted to improve the acclimatization rate of in vitro strawberry plantlets through bioreactor culture using the growth retardant flurprimidol. Different concentrations [0 (Control), 0.1, 0.5, 1.0, and 2.0 mg･L -1 ] of flurprimidol were added during bioreactor culture. After six weeks of treatments, various growth characteristics were investigated and in vitro plantlets were acclimated in the greenhouse. The growth rate of treated plantlets was much lower than that of control, and as the treatment concentration increased, the growth rate was much decreased. Shoots of plantlets treated with flurprimidol were shorter (2.2-3.7 cm) than those of control (7.9 cm). The number of roots per treated plant was around 11.6-34.2, compared with 51.8 in the control. Root length was also lower (0.88-3.08 cm) than control (4.36 cm). However, the number of new shoots and leaves increased in all treatments except for 2.0 mg･L -1 concentration. The root was partially decayed in 1.0 mg･L -1 concentration and was completely decayed in 2.0 mg･L -1 . The survival rate in 0.1 mg･L -1 and 0.5 mg･L -1 concentrations was 100% and 23.3% respectively. After four weeks of acclimatization, the plantlets restarted growth, and growth characteristics of shoots and roots recovered to the levels of control, except for fresh weight. Based on our results, a concentration of 0.1 mg･L -1 flurprimidol is appropriate for improvement of acclimatization rate of in vitro strawberry plantlets in bioreactor culture.

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Gibberellin Biosynthesis And Control

Cited by 190 publications

References 0 publications

Effects of Jasmonates on in vitro Tuberization in Several Potato Cultivars that Differ Greatly in Maturity

Effects of Jasmonates on in vitro Tuberization in Several Potato Cultivars that Differ Greatly in Maturity

Cloning gibberellin dioxygenase genes from pumpkin endosperm by heterologous expression of enzyme activities in Escherichia coli

Optimalization of Flurprimidol Concentration for Improvement of Acclimatization Rate and In Vitro Growth Inhibition in Bioreactor Culture of Strawberry Plantlets via Bioreactor Culture

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